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P59271

- R27AA_ARATH

UniProt

P59271 - R27AA_ARATH

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Protein

Ubiquitin-40S ribosomal protein S27a-1

Gene

RPS27AA

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, and DNA-damage responses. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity).By similarity
Ribosomal protein RSP27a-1 is a component of the 40S subunit of the ribosome.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri121 – 14424C4-typeAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. structural constituent of ribosome Source: InterPro

GO - Biological processi

  1. translation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein, Ribosomal protein

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_107429. Orc1 removal from chromatin.
REACT_185297. Separation of Sister Chromatids.
REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_187631. SRP-dependent cotranslational protein targeting to membrane.
REACT_187719. ER-Phagosome pathway.
REACT_187829. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
REACT_190928. Endosomal Sorting Complex Required For Transport (ESCRT).
REACT_190931. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_229741. Fanconi Anemia pathway.
REACT_231757. activated TAK1 mediates p38 MAPK activation.
REACT_232663. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_233701. Ribosomal scanning and start codon recognition.
REACT_235587. Hh ligand biogenesis disease.
REACT_238761. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_240263. Ubiquitin-dependent degradation of Cyclin D1.
REACT_240283. Peptide chain elongation.
REACT_241531. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_244054. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_244451. Formation of the ternary complex, and subsequently, the 43S complex.
REACT_245715. Cyclin D associated events in G1.
REACT_246136. Hedgehog ligand biogenesis.
REACT_248654. Eukaryotic Translation Termination.
REACT_252436. CDK-mediated phosphorylation and removal of Cdc6.
REACT_261170. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.
REACT_262398. Formation of a pool of free 40S subunits.
REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-40S ribosomal protein S27a-1
Cleaved into the following 2 chains:
Gene namesi
Name:RPS27AA
Synonyms:UBQ16
Ordered Locus Names:At1g23410
ORF Names:F26F24.28, F28C11.5
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G23410.

Subcellular locationi

Chain Ubiquitin : Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytosolic small ribosomal subunit Source: TAIR
  2. Golgi apparatus Source: TAIR
  3. nucleus Source: UniProtKB-KW
  4. plasma membrane Source: TAIR
  5. plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7676UbiquitinPRO_0000396871Add
BLAST
Chaini77 – 1568040S ribosomal protein S27a-1PRO_0000137674Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki11 – 11Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki29 – 29Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki33 – 33Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki48 – 48Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki63 – 63Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki76 – 76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)PROSITE-ProRule annotation

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP59271.
PRIDEiP59271.

Expressioni

Gene expression databases

ExpressionAtlasiP59271. baseline and differential.

Interactioni

Subunit structurei

Ribosomal protein RSP27a-1 is part of the 40S ribosomal subunit.By similarity

Protein-protein interaction databases

BioGridi24188. 1 interaction.
IntActiP59271. 6 interactions.

Structurei

3D structure databases

ProteinModelPortaliP59271.
SMRiP59271. Positions 1-76, 83-152.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 7676Ubiquitin-likePROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi77 – 9923Lys-rich (highly basic)Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the ubiquitin family.Curated
In the C-terminal section; belongs to the ribosomal protein S27Ae family.Curated
Contains 1 ubiquitin-like domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri121 – 14424C4-typeAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG5272.
InParanoidiP59271.
KOiK02977.
OMAiECPSVSC.

Family and domain databases

InterProiIPR002906. Ribosomal_S27a.
IPR011332. Ribosomal_zn-bd.
IPR019956. Ubiquitin.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
[Graphical view]
PfamiPF01599. Ribosomal_S27. 1 hit.
PF00240. ubiquitin. 1 hit.
[Graphical view]
PRINTSiPR00348. UBIQUITIN.
SMARTiSM00213. UBQ. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF57829. SSF57829. 1 hit.
PROSITEiPS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P59271-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQIFVKTLTG KTITLEVESS DTIDNVKAKI QDKEGIPPDQ QRLIFAGKQL
60 70 80 90 100
EDGRTLADYN IQKESTLHLV LRLRGGAKKR KKKTYTKPKK IKHTHKKVKL
110 120 130 140 150
AVLQFYKVDG SGKVQRLKKE CPSVSCGPGT FMASHFDRHY CGKCGTTYVF

KKADEE
Length:156
Mass (Da):17,672
Last modified:August 10, 2010 - v2
Checksum:i95FBD9561FD1A25E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005292 Genomic DNA. Translation: AAF87001.1.
AC007945 Genomic DNA. Translation: AAF79581.1.
CP002684 Genomic DNA. Translation: AEE30384.1.
BT024763 mRNA. Translation: ABD59101.1.
AY085389 mRNA. Translation: AAM62617.1.
PIRiH86367.
RefSeqiNP_173755.1. NM_102190.2.
UniGeneiAt.41552.

Genome annotation databases

EnsemblPlantsiAT1G23410.1; AT1G23410.1; AT1G23410.
GeneIDi838949.
KEGGiath:AT1G23410.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC005292 Genomic DNA. Translation: AAF87001.1 .
AC007945 Genomic DNA. Translation: AAF79581.1 .
CP002684 Genomic DNA. Translation: AEE30384.1 .
BT024763 mRNA. Translation: ABD59101.1 .
AY085389 mRNA. Translation: AAM62617.1 .
PIRi H86367.
RefSeqi NP_173755.1. NM_102190.2.
UniGenei At.41552.

3D structure databases

ProteinModelPortali P59271.
SMRi P59271. Positions 1-76, 83-152.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 24188. 1 interaction.
IntActi P59271. 6 interactions.

Proteomic databases

PaxDbi P59271.
PRIDEi P59271.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G23410.1 ; AT1G23410.1 ; AT1G23410 .
GeneIDi 838949.
KEGGi ath:AT1G23410.

Organism-specific databases

TAIRi AT1G23410.

Phylogenomic databases

eggNOGi COG5272.
InParanoidi P59271.
KOi K02977.
OMAi ECPSVSC.

Enzyme and pathway databases

Reactomei REACT_106068. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_107429. Orc1 removal from chromatin.
REACT_185297. Separation of Sister Chromatids.
REACT_185305. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_187631. SRP-dependent cotranslational protein targeting to membrane.
REACT_187719. ER-Phagosome pathway.
REACT_187829. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).
REACT_187848. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_187954. Senescence-Associated Secretory Phenotype (SASP).
REACT_190928. Endosomal Sorting Complex Required For Transport (ESCRT).
REACT_190931. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
REACT_229741. Fanconi Anemia pathway.
REACT_231757. activated TAK1 mediates p38 MAPK activation.
REACT_232663. GTP hydrolysis and joining of the 60S ribosomal subunit.
REACT_233701. Ribosomal scanning and start codon recognition.
REACT_235587. Hh ligand biogenesis disease.
REACT_238761. L13a-mediated translational silencing of Ceruloplasmin expression.
REACT_240263. Ubiquitin-dependent degradation of Cyclin D1.
REACT_240283. Peptide chain elongation.
REACT_241531. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_244054. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_244451. Formation of the ternary complex, and subsequently, the 43S complex.
REACT_245715. Cyclin D associated events in G1.
REACT_246136. Hedgehog ligand biogenesis.
REACT_248654. Eukaryotic Translation Termination.
REACT_252436. CDK-mediated phosphorylation and removal of Cdc6.
REACT_261170. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.
REACT_262398. Formation of a pool of free 40S subunits.
REACT_94347. APC/C:Cdc20 mediated degradation of Securin.

Gene expression databases

ExpressionAtlasi P59271. baseline and differential.

Family and domain databases

InterProi IPR002906. Ribosomal_S27a.
IPR011332. Ribosomal_zn-bd.
IPR019956. Ubiquitin.
IPR000626. Ubiquitin-like.
IPR029071. Ubiquitin-rel_dom.
IPR019954. Ubiquitin_CS.
[Graphical view ]
Pfami PF01599. Ribosomal_S27. 1 hit.
PF00240. ubiquitin. 1 hit.
[Graphical view ]
PRINTSi PR00348. UBIQUITIN.
SMARTi SM00213. UBQ. 1 hit.
[Graphical view ]
SUPFAMi SSF54236. SSF54236. 1 hit.
SSF57829. SSF57829. 1 hit.
PROSITEi PS00299. UBIQUITIN_1. 1 hit.
PS50053. UBIQUITIN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Arabidopsis ORF clones."
    Shinn P., Chen H., Kim C.J., Ecker J.R.
    Submitted (MAR-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "The organization of cytoplasmic ribosomal protein genes in the Arabidopsis genome."
    Barakat A., Szick-Miranda K., Chang I.-F., Guyot R., Blanc G., Cooke R., Delseny M., Bailey-Serres J.
    Plant Physiol. 127:398-415(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.
  6. "Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants."
    Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K.
    Mol. Cell. Proteomics 6:601-610(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-11; LYS-29; LYS-33; LYS-48 AND LYS-63.
    Strain: cv. Landsberg erecta.

Entry informationi

Entry nameiR27AA_ARATH
AccessioniPrimary (citable) accession number: P59271
Secondary accession number(s): O80715
, P59263, Q29PZ3, Q38875, Q9LDJ2, Q9LYW1, Q9M0W3, Q9M1P9, Q9S7X3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: August 10, 2010
Last modified: November 26, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Ubiquitin is encoded by 16 different genes. Ubiquitin is generally synthesized as a polyubiquitin precursor with tandem head to tail repeats. Often, there is one to three additional amino acids after the last repeat, removed in the mature protein. Alternatively, ubiquitin extension protein is synthesized as a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27A) or to a ubiquitin-related protein (either RUB1 or RUB2). Following translation, extension protein is cleaved from ubiquitin.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3