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P59270 (B3GA2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2

EC=2.4.1.135
Alternative name(s):
Beta-1,3-glucuronyltransferase 2
GlcAT-D
UDP-glucuronosyltransferase S
Short name=GlcAT-S
Short name=Glucuronosyltransferase S
Gene names
Name:B3gat2
Synonyms:Glcats
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on both glycolipids and glycoproteins.

Catalytic activity

UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein.

Cofactor

Manganese.

Pathway

Protein modification; protein glycosylation.

Subunit structure

Homodimer Potential.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Tissue specificity

Expressed in brain, but not in liver and kidney.

Sequence similarities

Belongs to the glycosyltransferase 43 family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P59270-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P59270-2)

The sequence of this isoform differs from the canonical sequence as follows:
     248-302: FAVSLQVILS...NCTKVLVWHT → EQNAWDICPC...VQVEVDFRSG
     303-324: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 324324Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2
PRO_0000195173

Regions

Topological domain1 – 22Cytoplasmic Potential
Transmembrane3 – 2321Helical; Signal-anchor for type II membrane protein; Potential
Topological domain24 – 324301Lumenal Potential
Nucleotide binding88 – 903UDP-glucuronate binding By similarity
Nucleotide binding186 – 1883UDP-glucuronate binding By similarity
Nucleotide binding301 – 3033UDP-glucuronate binding By similarity
Region235 – 24410Interaction with galactose moiety of substrate glycoprotein By similarity

Sites

Active site2741Proton donor/acceptor By similarity
Metal binding1881Manganese By similarity
Binding site1191UDP-glucuronate By similarity
Binding site1561UDP-glucuronate By similarity
Binding site1611UDP-glucuronate By similarity
Site2191Interaction with galactose moiety of substrate glycoprotein By similarity
Site3111Interaction with galactose moiety of substrate glycoprotein By similarity

Amino acid modifications

Glycosylation681N-linked (GlcNAc...) Potential
Glycosylation2931N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence248 – 30255FAVSL…LVWHT → EQNAWDICPCRMGPRRNWER PIFIKPLSVLHSSEEILIFK IGSLMVQVEVDFRSG in isoform 2.
VSP_001796
Alternative sequence303 – 32422Missing in isoform 2.
VSP_001797

Experimental info

Sequence conflict2911A → V in BAC20343. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 1, 2003. Version 1.
Checksum: 675C549D3787B957

FASTA32437,132
        10         20         30         40         50         60 
MKSALCSRFF ILLPWILIVI IMLDVDPRRP APQLTSRPYF SPHAVGCGGS RVPLRRSSPG 

        70         80         90        100        110        120 
RDAAEKRNES RPQLQPEPRL PTIYAITPTY SRPVQKAELT RLANTFRQVA QLHWILVEDR 

       130        140        150        160        170        180 
ATRSELVSSF LARAGLPNTH LHVPTPRRYK RPWLPRATEQ RNAGLAWLRQ RHQHQSAQPG 

       190        200        210        220        230        240 
VLFFADDDNT YSLELFQEMR TTRKVSVWPV GLVGGRRYER PLVKNGKVVG WYTGWREDRP 

       250        260        270        280        290        300 
FAIDMAGFAV SLQVILSNPK AVFKRRGSQP GMQESDFLKQ ITTVEELEPK ASNCTKVLVW 

       310        320 
HTRTEKVNLA NEPKYHLDTV NIEV 

« Hide

Isoform 2 [UniParc].

Checksum: 1DFD4FA45506B311
Show »

FASTA30234,808

References

« Hide 'large scale' references
[1]"cDNA cloning, genomic structure and chromosomal mapping of the mouse glucuronyltransferase-S involved in the biosynthesis of the HNK-1 carbohydrate epitope."
Imiya K., Ishizaki T., Seiki T., Saito F., Inazawa J., Oka S., Kawasaki T.
Gene 296:29-36(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: BALB/c.
Tissue: Brain.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Embryonic head and Kidney.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB055902 mRNA. Translation: BAC20343.1.
AK048146 mRNA. Translation: BAC33257.1.
AK052640 mRNA. Translation: BAC35075.1.
BC056368 mRNA. Translation: AAH56368.1.
BC058082 mRNA. Translation: AAH58082.1.
CCDSCCDS14850.1. [P59270-1]
RefSeqNP_742122.2. NM_172124.2. [P59270-1]
UniGeneMm.471661.

3D structure databases

ProteinModelPortalP59270.
SMRP59270. Positions 79-324.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4996887.

Protein family/group databases

CAZyGT43. Glycosyltransferase Family 43.

Proteomic databases

PRIDEP59270.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000063663; ENSMUSP00000066582; ENSMUSG00000026156. [P59270-1]
ENSMUST00000140583; ENSMUSP00000117089; ENSMUSG00000026156. [P59270-2]
GeneID280645.
KEGGmmu:280645.
UCSCuc007amd.1. mouse. [P59270-2]
uc007ame.1. mouse. [P59270-1]

Organism-specific databases

CTD135152.
MGIMGI:2389490. B3gat2.

Phylogenomic databases

eggNOGNOG292627.
GeneTreeENSGT00390000017640.
HOVERGENHBG050650.
InParanoidP59270.
KOK10157.
OMAFILLPWV.
TreeFamTF313522.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

BgeeP59270.
GenevestigatorP59270.

Family and domain databases

Gene3D3.90.550.10. 1 hit.
InterProIPR005027. Glyco_trans_43.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERPTHR10896. PTHR10896. 1 hit.
PfamPF03360. Glyco_transf_43. 1 hit.
[Graphical view]
SUPFAMSSF53448. SSF53448. 1 hit.
ProtoNetSearch...

Other

NextBio394065.
PROP59270.
SOURCESearch...

Entry information

Entry nameB3GA2_MOUSE
AccessionPrimary (citable) accession number: P59270
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: February 1, 2003
Last modified: July 9, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot