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Protein

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2

Gene

B3gat2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on both glycolipids and glycoproteins.

Catalytic activityi

UDP-alpha-D-glucuronate + [protein]-3-O-(beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl)-L-serine = UDP + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine.By similarity

Cofactori

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei119UDP-glucuronateBy similarity1
Binding sitei156UDP-glucuronateBy similarity1
Binding sitei161UDP-glucuronateBy similarity1
Metal bindingi188ManganeseBy similarity1
Active sitei274Proton donor/acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi88 – 90UDP-glucuronate bindingBy similarity3
Nucleotide bindingi186 – 188UDP-glucuronate bindingBy similarity3
Nucleotide bindingi301 – 303UDP-glucuronate bindingBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1971475. A tetrasaccharide linker sequence is required for GAG synthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT43. Glycosyltransferase Family 43.

Names & Taxonomyi

Protein namesi
Recommended name:
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2 (EC:2.4.1.135By similarity)
Alternative name(s):
Beta-1,3-glucuronyltransferase 2
GlcAT-D
UDP-glucuronosyltransferase S
Short name:
GlcAT-S
Short name:
Glucuronosyltransferase S
Gene namesi
Name:B3gat2
Synonyms:Glcats
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2389490. B3gat2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 2CytoplasmicSequence analysis2
Transmembranei3 – 23Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini24 – 324LumenalSequence analysisAdd BLAST301

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001951731 – 324Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2Add BLAST324

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi68N-linked (GlcNAc...)Sequence analysis1
Glycosylationi293N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP59270.
PaxDbiP59270.
PRIDEiP59270.

PTM databases

PhosphoSitePlusiP59270.

Expressioni

Tissue specificityi

Expressed in brain, but not in liver and kidney.

Gene expression databases

BgeeiENSMUSG00000026156.
ExpressionAtlasiP59270. baseline and differential.
GenevisibleiP59270. MM.

Interactioni

Subunit structurei

Homodimer.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei219Interaction with galactose moiety of substrate glycoproteinBy similarity1
Sitei311Interaction with galactose moiety of substrate glycoproteinBy similarity1

Protein-protein interaction databases

MINTiMINT-4996887.
STRINGi10090.ENSMUSP00000066582.

Structurei

3D structure databases

ProteinModelPortaliP59270.
SMRiP59270.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni235 – 244Interaction with galactose moiety of substrate glycoproteinBy similarity10

Sequence similaritiesi

Belongs to the glycosyltransferase 43 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1476. Eukaryota.
ENOG410XP79. LUCA.
GeneTreeiENSGT00390000017640.
HOVERGENiHBG050650.
InParanoidiP59270.
KOiK10157.
OMAiEEMRTTH.
OrthoDBiEOG091G0G8P.
TreeFamiTF313522.

Family and domain databases

CDDicd00218. GlcAT-I. 1 hit.
Gene3Di3.90.550.10. 1 hit.
InterProiIPR005027. Glyco_trans_43.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10896. PTHR10896. 1 hit.
PfamiPF03360. Glyco_transf_43. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P59270-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKSALCSRFF ILLPWILIVI IMLDVDPRRP APQLTSRPYF SPHAVGCGGS
60 70 80 90 100
RVPLRRSSPG RDAAEKRNES RPQLQPEPRL PTIYAITPTY SRPVQKAELT
110 120 130 140 150
RLANTFRQVA QLHWILVEDR ATRSELVSSF LARAGLPNTH LHVPTPRRYK
160 170 180 190 200
RPWLPRATEQ RNAGLAWLRQ RHQHQSAQPG VLFFADDDNT YSLELFQEMR
210 220 230 240 250
TTRKVSVWPV GLVGGRRYER PLVKNGKVVG WYTGWREDRP FAIDMAGFAV
260 270 280 290 300
SLQVILSNPK AVFKRRGSQP GMQESDFLKQ ITTVEELEPK ASNCTKVLVW
310 320
HTRTEKVNLA NEPKYHLDTV NIEV
Length:324
Mass (Da):37,132
Last modified:February 1, 2003 - v1
Checksum:i675C549D3787B957
GO
Isoform 2 (identifier: P59270-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     248-302: FAVSLQVILS...NCTKVLVWHT → EQNAWDICPC...VQVEVDFRSG
     303-324: Missing.

Note: No experimental confirmation available.
Show »
Length:302
Mass (Da):34,808
Checksum:i1DFD4FA45506B311
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti291A → V in BAC20343 (PubMed:12383500).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001796248 – 302FAVSL…LVWHT → EQNAWDICPCRMGPRRNWER PIFIKPLSVLHSSEEILIFK IGSLMVQVEVDFRSG in isoform 2. 1 PublicationAdd BLAST55
Alternative sequenceiVSP_001797303 – 324Missing in isoform 2. 1 PublicationAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB055902 mRNA. Translation: BAC20343.1.
AK048146 mRNA. Translation: BAC33257.1.
AK052640 mRNA. Translation: BAC35075.1.
BC056368 mRNA. Translation: AAH56368.1.
BC058082 mRNA. Translation: AAH58082.1.
CCDSiCCDS14850.1. [P59270-1]
RefSeqiNP_742122.2. NM_172124.2. [P59270-1]
UniGeneiMm.471661.

Genome annotation databases

EnsembliENSMUST00000063663; ENSMUSP00000066582; ENSMUSG00000026156. [P59270-1]
ENSMUST00000140583; ENSMUSP00000117089; ENSMUSG00000026156. [P59270-2]
GeneIDi280645.
KEGGimmu:280645.
UCSCiuc007amd.1. mouse. [P59270-2]
uc007ame.1. mouse. [P59270-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB055902 mRNA. Translation: BAC20343.1.
AK048146 mRNA. Translation: BAC33257.1.
AK052640 mRNA. Translation: BAC35075.1.
BC056368 mRNA. Translation: AAH56368.1.
BC058082 mRNA. Translation: AAH58082.1.
CCDSiCCDS14850.1. [P59270-1]
RefSeqiNP_742122.2. NM_172124.2. [P59270-1]
UniGeneiMm.471661.

3D structure databases

ProteinModelPortaliP59270.
SMRiP59270.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4996887.
STRINGi10090.ENSMUSP00000066582.

Protein family/group databases

CAZyiGT43. Glycosyltransferase Family 43.

PTM databases

PhosphoSitePlusiP59270.

Proteomic databases

MaxQBiP59270.
PaxDbiP59270.
PRIDEiP59270.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000063663; ENSMUSP00000066582; ENSMUSG00000026156. [P59270-1]
ENSMUST00000140583; ENSMUSP00000117089; ENSMUSG00000026156. [P59270-2]
GeneIDi280645.
KEGGimmu:280645.
UCSCiuc007amd.1. mouse. [P59270-2]
uc007ame.1. mouse. [P59270-1]

Organism-specific databases

CTDi135152.
MGIiMGI:2389490. B3gat2.

Phylogenomic databases

eggNOGiKOG1476. Eukaryota.
ENOG410XP79. LUCA.
GeneTreeiENSGT00390000017640.
HOVERGENiHBG050650.
InParanoidiP59270.
KOiK10157.
OMAiEEMRTTH.
OrthoDBiEOG091G0G8P.
TreeFamiTF313522.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-MMU-1971475. A tetrasaccharide linker sequence is required for GAG synthesis.

Miscellaneous databases

PROiP59270.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026156.
ExpressionAtlasiP59270. baseline and differential.
GenevisibleiP59270. MM.

Family and domain databases

CDDicd00218. GlcAT-I. 1 hit.
Gene3Di3.90.550.10. 1 hit.
InterProiIPR005027. Glyco_trans_43.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR10896. PTHR10896. 1 hit.
PfamiPF03360. Glyco_transf_43. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiB3GA2_MOUSE
AccessioniPrimary (citable) accession number: P59270
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: February 1, 2003
Last modified: November 30, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.