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P59270

- B3GA2_MOUSE

UniProt

P59270 - B3GA2_MOUSE

Protein

Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2

Gene

B3gat2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Feb 2003)
      Previous versions | rss
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    Functioni

    Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on both glycolipids and glycoproteins.

    Catalytic activityi

    UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein.

    Cofactori

    Manganese.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei119 – 1191UDP-glucuronateBy similarity
    Binding sitei156 – 1561UDP-glucuronateBy similarity
    Binding sitei161 – 1611UDP-glucuronateBy similarity
    Metal bindingi188 – 1881ManganeseBy similarity
    Sitei219 – 2191Interaction with galactose moiety of substrate glycoproteinBy similarity
    Active sitei274 – 2741Proton donor/acceptorBy similarity
    Sitei311 – 3111Interaction with galactose moiety of substrate glycoproteinBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi88 – 903UDP-glucuronate bindingBy similarity
    Nucleotide bindingi186 – 1883UDP-glucuronate bindingBy similarity
    Nucleotide bindingi301 – 3033UDP-glucuronate bindingBy similarity

    GO - Molecular functioni

    1. galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity Source: UniProtKB
    2. glucuronosyltransferase activity Source: MGI
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_196489. A tetrasaccharide linker sequence is required for GAG synthesis.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT43. Glycosyltransferase Family 43.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2 (EC:2.4.1.135)
    Alternative name(s):
    Beta-1,3-glucuronyltransferase 2
    GlcAT-D
    UDP-glucuronosyltransferase S
    Short name:
    GlcAT-S
    Short name:
    Glucuronosyltransferase S
    Gene namesi
    Name:B3gat2
    Synonyms:Glcats
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:2389490. B3gat2.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 324324Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2PRO_0000195173Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi68 – 681N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi293 – 2931N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiP59270.

    Expressioni

    Tissue specificityi

    Expressed in brain, but not in liver and kidney.

    Gene expression databases

    BgeeiP59270.
    GenevestigatoriP59270.

    Interactioni

    Subunit structurei

    Homodimer.Curated

    Protein-protein interaction databases

    MINTiMINT-4996887.

    Structurei

    3D structure databases

    ProteinModelPortaliP59270.
    SMRiP59270. Positions 79-324.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 22CytoplasmicSequence Analysis
    Topological domaini24 – 324301LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei3 – 2321Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni235 – 24410Interaction with galactose moiety of substrate glycoproteinBy similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 43 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG292627.
    GeneTreeiENSGT00390000017640.
    HOVERGENiHBG050650.
    InParanoidiP59270.
    KOiK10157.
    OMAiFILLPWV.
    TreeFamiTF313522.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR005027. Glyco_trans_43.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR10896. PTHR10896. 1 hit.
    PfamiPF03360. Glyco_transf_43. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P59270-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKSALCSRFF ILLPWILIVI IMLDVDPRRP APQLTSRPYF SPHAVGCGGS    50
    RVPLRRSSPG RDAAEKRNES RPQLQPEPRL PTIYAITPTY SRPVQKAELT 100
    RLANTFRQVA QLHWILVEDR ATRSELVSSF LARAGLPNTH LHVPTPRRYK 150
    RPWLPRATEQ RNAGLAWLRQ RHQHQSAQPG VLFFADDDNT YSLELFQEMR 200
    TTRKVSVWPV GLVGGRRYER PLVKNGKVVG WYTGWREDRP FAIDMAGFAV 250
    SLQVILSNPK AVFKRRGSQP GMQESDFLKQ ITTVEELEPK ASNCTKVLVW 300
    HTRTEKVNLA NEPKYHLDTV NIEV 324
    Length:324
    Mass (Da):37,132
    Last modified:February 1, 2003 - v1
    Checksum:i675C549D3787B957
    GO
    Isoform 2 (identifier: P59270-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         248-302: FAVSLQVILS...NCTKVLVWHT → EQNAWDICPC...VQVEVDFRSG
         303-324: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:302
    Mass (Da):34,808
    Checksum:i1DFD4FA45506B311
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti291 – 2911A → V in BAC20343. (PubMed:12383500)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei248 – 30255FAVSL…LVWHT → EQNAWDICPCRMGPRRNWER PIFIKPLSVLHSSEEILIFK IGSLMVQVEVDFRSG in isoform 2. 1 PublicationVSP_001796Add
    BLAST
    Alternative sequencei303 – 32422Missing in isoform 2. 1 PublicationVSP_001797Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB055902 mRNA. Translation: BAC20343.1.
    AK048146 mRNA. Translation: BAC33257.1.
    AK052640 mRNA. Translation: BAC35075.1.
    BC056368 mRNA. Translation: AAH56368.1.
    BC058082 mRNA. Translation: AAH58082.1.
    CCDSiCCDS14850.1. [P59270-1]
    RefSeqiNP_742122.2. NM_172124.2. [P59270-1]
    UniGeneiMm.471661.

    Genome annotation databases

    EnsembliENSMUST00000063663; ENSMUSP00000066582; ENSMUSG00000026156. [P59270-1]
    ENSMUST00000140583; ENSMUSP00000117089; ENSMUSG00000026156. [P59270-2]
    GeneIDi280645.
    KEGGimmu:280645.
    UCSCiuc007amd.1. mouse. [P59270-2]
    uc007ame.1. mouse. [P59270-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB055902 mRNA. Translation: BAC20343.1 .
    AK048146 mRNA. Translation: BAC33257.1 .
    AK052640 mRNA. Translation: BAC35075.1 .
    BC056368 mRNA. Translation: AAH56368.1 .
    BC058082 mRNA. Translation: AAH58082.1 .
    CCDSi CCDS14850.1. [P59270-1 ]
    RefSeqi NP_742122.2. NM_172124.2. [P59270-1 ]
    UniGenei Mm.471661.

    3D structure databases

    ProteinModelPortali P59270.
    SMRi P59270. Positions 79-324.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4996887.

    Protein family/group databases

    CAZyi GT43. Glycosyltransferase Family 43.

    Proteomic databases

    PRIDEi P59270.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000063663 ; ENSMUSP00000066582 ; ENSMUSG00000026156 . [P59270-1 ]
    ENSMUST00000140583 ; ENSMUSP00000117089 ; ENSMUSG00000026156 . [P59270-2 ]
    GeneIDi 280645.
    KEGGi mmu:280645.
    UCSCi uc007amd.1. mouse. [P59270-2 ]
    uc007ame.1. mouse. [P59270-1 ]

    Organism-specific databases

    CTDi 135152.
    MGIi MGI:2389490. B3gat2.

    Phylogenomic databases

    eggNOGi NOG292627.
    GeneTreei ENSGT00390000017640.
    HOVERGENi HBG050650.
    InParanoidi P59270.
    KOi K10157.
    OMAi FILLPWV.
    TreeFami TF313522.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_196489. A tetrasaccharide linker sequence is required for GAG synthesis.

    Miscellaneous databases

    NextBioi 394065.
    PROi P59270.
    SOURCEi Search...

    Gene expression databases

    Bgeei P59270.
    Genevestigatori P59270.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR005027. Glyco_trans_43.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR10896. PTHR10896. 1 hit.
    Pfami PF03360. Glyco_transf_43. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning, genomic structure and chromosomal mapping of the mouse glucuronyltransferase-S involved in the biosynthesis of the HNK-1 carbohydrate epitope."
      Imiya K., Ishizaki T., Seiki T., Saito F., Inazawa J., Oka S., Kawasaki T.
      Gene 296:29-36(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: BALB/c.
      Tissue: Brain.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J.
      Tissue: Embryonic head and Kidney.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiB3GA2_MOUSE
    AccessioniPrimary (citable) accession number: P59270
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 2003
    Last sequence update: February 1, 2003
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3