P59270 (B3GA2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2
EC=2.4.1.135

Alternative name(s):
Beta-1,3-glucuronyltransferase 2
GlcAT-D
UDP-glucuronosyltransferase S
Short name=GlcAT-S
Short name=Glucuronosyltransferase S

Gene names

Name:	B3gat2
Synonyms:	Glcats

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on both glycolipids and glycoproteins.
Catalytic activity	UDP-glucuronate + 3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein = UDP + 3-beta-D-glucuronosyl-3-beta-D-galactosyl-4-beta-D-galactosyl-O-beta-D-xylosylprotein.
Cofactor	Manganese.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Homodimer Potential.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.
Tissue specificity	Expressed in brain, but not in liver and kidney.
Sequence similarities	Belongs to the glycosyltransferase 43 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Manganese Metal-binding
Molecular function	Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P59270-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P59270-2)

The sequence of this isoform differs from the canonical sequence as follows:
     248-302: FAVSLQVILS...NCTKVLVWHT → EQNAWDICPC...VQVEVDFRSG
     303-324: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 2		PRO_0000195173
Regions
	Topological domain	1 – 2	2	Cytoplasmic Potential
	Transmembrane	3 – 23	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	24 – 324	301	Lumenal Potential
	Nucleotide binding	88 – 90	3	UDP-glucuronate binding By similarity
	Nucleotide binding	186 – 188	3	UDP-glucuronate binding By similarity
	Nucleotide binding	301 – 303	3	UDP-glucuronate binding By similarity
	Region	235 – 244	10	Interaction with galactose moiety of substrate glycoprotein By similarity
Sites
	Active site	274	1	Proton donor/acceptor By similarity
	Metal binding	188	1	Manganese By similarity
	Binding site	119	1	UDP-glucuronate By similarity
	Binding site	156	1	UDP-glucuronate By similarity
	Binding site	161	1	UDP-glucuronate By similarity
	Site	219	1	Interaction with galactose moiety of substrate glycoprotein By similarity
	Site	311	1	Interaction with galactose moiety of substrate glycoprotein By similarity
Amino acid modifications
	Glycosylation	68	1	N-linked (GlcNAc...) Potential
	Glycosylation	293	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	248 – 302	55	FAVSL…LVWHT → EQNAWDICPCRMGPRRNWER PIFIKPLSVLHSSEEILIFK IGSLMVQVEVDFRSG in isoform 2.		VSP_001796
	Alternative sequence	303 – 324	22	Missing in isoform 2.		VSP_001797
Experimental info
	Sequence conflict	291	1	A → V in BAC20343. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 2003. Version 1. Checksum: 675C549D3787B957	FASTA	324	37,132
	10 20 30 40 50 60 MKSALCSRFF ILLPWILIVI IMLDVDPRRP APQLTSRPYF SPHAVGCGGS RVPLRRSSPG 70 80 90 100 110 120 RDAAEKRNES RPQLQPEPRL PTIYAITPTY SRPVQKAELT RLANTFRQVA QLHWILVEDR 130 140 150 160 170 180 ATRSELVSSF LARAGLPNTH LHVPTPRRYK RPWLPRATEQ RNAGLAWLRQ RHQHQSAQPG 190 200 210 220 230 240 VLFFADDDNT YSLELFQEMR TTRKVSVWPV GLVGGRRYER PLVKNGKVVG WYTGWREDRP 250 260 270 280 290 300 FAIDMAGFAV SLQVILSNPK AVFKRRGSQP GMQESDFLKQ ITTVEELEPK ASNCTKVLVW 310 320 HTRTEKVNLA NEPKYHLDTV NIEV
	Isoform 2 [UniParc]. Checksum: 1DFD4FA45506B311	FASTA	302	34,808
	10 20 30 40 50 60 MKSALCSRFF ILLPWILIVI IMLDVDPRRP APQLTSRPYF SPHAVGCGGS RVPLRRSSPG 70 80 90 100 110 120 RDAAEKRNES RPQLQPEPRL PTIYAITPTY SRPVQKAELT RLANTFRQVA QLHWILVEDR 130 140 150 160 170 180 ATRSELVSSF LARAGLPNTH LHVPTPRRYK RPWLPRATEQ RNAGLAWLRQ RHQHQSAQPG 190 200 210 220 230 240 VLFFADDDNT YSLELFQEMR TTRKVSVWPV GLVGGRRYER PLVKNGKVVG WYTGWREDRP 250 260 270 280 290 300 FAIDMAGEQN AWDICPCRMG PRRNWERPIF IKPLSVLHSS EEILIFKIGS LMVQVEVDFR SG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning, genomic structure and chromosomal mapping of the mouse glucuronyltransferase-S involved in the biosynthesis of the HNK-1 carbohydrate epitope." Imiya K., Ishizaki T., Seiki T., Saito F., Inazawa J., Oka S., Kawasaki T. Gene 296:29-36(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: BALB/c. Tissue: Brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Embryonic head and Kidney.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB055902 mRNA. Translation: BAC20343.1. AK048146 mRNA. Translation: BAC33257.1. AK052640 mRNA. Translation: BAC35075.1. BC056368 mRNA. Translation: AAH56368.1. BC058082 mRNA. Translation: AAH58082.1.
IPI	IPI00230585. IPI00323546.
RefSeq	NP_742122.2. NM_172124.2.
UniGene	Mm.471661.
3D structure databases
ProteinModelPortal	P59270.
SMR	P59270. Positions 79-324.
ModBase	Search...
Protein family/group databases
CAZy	GT43. Glycosyltransferase Family 43.
Proteomic databases
PRIDE	P59270.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000063663; ENSMUSP00000066582; ENSMUSG00000026156. ENSMUST00000140583; ENSMUSP00000117089; ENSMUSG00000026156.
GeneID	280645.
KEGG	mmu:280645.
UCSC	uc007amd.1. mouse.
Organism-specific databases
CTD	135152.
MGI	MGI:2389490. B3gat2.
Phylogenomic databases
eggNOG	NOG292627.
GeneTree	ENSGT00390000017640.
HOVERGEN	HBG050650.
InParanoid	P59270.
KO	K10157.
OMA	FILLPWV.
OrthoDB	EOG4CRM0N.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
ArrayExpress	P59270.
Bgee	P59270.
Genevestigator	P59270.
GermOnline	ENSMUSG00000026156. Mus musculus.
Family and domain databases
InterPro	IPR005027. Glyco_trans_43. [Graphical view]
PANTHER	PTHR10896. PTHR10896. 1 hit.
Pfam	PF03360. Glyco_transf_43. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	394065.
SOURCE	Search...

Entry information

Entry name

B3GA2_MOUSE

Accession

Primary (citable) accession number: P59270

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2003
Last sequence update:	February 1, 2003
Last modified:	April 3, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program