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P59241

- AURKA_RAT

UniProt

P59241 - AURKA_RAT

Protein

Aurora kinase A

Gene

Aurka

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (27 Jan 2003)
      Previous versions | rss
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    Functioni

    Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizating p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity By similarity. Necessary for proper cilia disassembly prior to mitosis By similarity. Interacts with SIRT2 By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Activation of CDK1, appears to be an upstream event of AURKA activation. Phosphatase inhibitor-2 (PPP1R2) and TPX2 act also as activators. Phosphatase inhibitor-2 (PPP1R2) and TPX2 act also as activators. Inactivated by the G2 checkpoint. Inhibited by GADD45A and p53/TP53, and through dephosphorylation by protein phosphatase type 1 (PP1). MLN8054 is also a potent and selective inhibitor By similarity. Activated during the early phase of cilia disassembly in the presence of PIFO By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei136 – 1361ATP; via amide nitrogenPROSITE-ProRule annotation
    Binding sitei155 – 1551ATPPROSITE-ProRule annotation
    Active sitei249 – 2491Proton acceptorPROSITE-ProRule annotation
    Binding sitei267 – 2671ATPPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi203 – 2064ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein kinase activity Source: UniProtKB
    3. protein serine/threonine kinase activity Source: RGD

    GO - Biological processi

    1. cell projection organization Source: UniProtKB-KW
    2. mitotic nuclear division Source: UniProtKB-KW
    3. protein phosphorylation Source: RGD
    4. response to estradiol Source: RGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Cilium biogenesis/degradation, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aurora kinase A (EC:2.7.11.1)
    Alternative name(s):
    Aurora 2
    Aurora/IPL1-related kinase 1
    Short name:
    ARK-1
    Short name:
    Aurora-related kinase 1
    Serine/threonine-protein kinase 6
    Serine/threonine-protein kinase aurora-A
    Short name:
    ratAurA
    Gene namesi
    Name:Aurka
    Synonyms:Aik, Airk, Ark1, Aura, Ayk1, Btak, Iak1, Stk15, Stk6
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi628895. Aurka.

    Subcellular locationi

    Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytoskeletonspindle pole By similarity
    Note: Localizes on centrosomes in interphase cells and at each spindle pole in mitosis. Associates with both the pericentriolar material (PCM) and centrioles. Detected at the neurite hillock in developing neurons. Colocalized with SIRT2 at centrosome By similarity.By similarity

    GO - Cellular componenti

    1. centrosome Source: RGD
    2. cytoplasm Source: UniProtKB-KW
    3. microtubule Source: UniProtKB-KW
    4. spindle Source: RGD
    5. spindle pole Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Microtubule

    Pathology & Biotechi

    Keywords - Diseasei

    Proto-oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 397397Aurora kinase APRO_0000086694Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei40 – 401PhosphoserineBy similarity
    Modified residuei50 – 501PhosphoserineBy similarity
    Modified residuei280 – 2801PhosphothreonineBy similarity
    Modified residuei281 – 2811PhosphothreonineBy similarity
    Modified residuei335 – 3351Phosphoserine; by PKA and PAKBy similarity

    Post-translational modificationi

    Activated by phosphorylation at Thr-281; this brings about a change in the conformation of the activation segment. Phosphorylation at Thr-281 varies during the cell cycle and is highest during M phase. Autophosphorylated at Thr-281 upon TPX2 binding. Thr-281 can be phosphorylated by several kinases, including PAK and PKA. Protein phosphatase type 1 (PP1) binds AURKA and inhibits its activity by dephosphorylating Thr-281 during mitosis. Phosphorylation at Ser-335 decreases the kinase activity. PPP2CA controls degradation by dephosphorylating Ser-52 at the end of mitosis By similarity. Phosphorylated in embryonic brain neurons.By similarity1 Publication
    Ubiquitinated by the anaphase-promoting complex (APC), leading to its degradation by the proteasome By similarity. Ubiquitinated by CHFR, leading to its degradation by the proteasome. Ubiquitinated by the E3 ubiquitin-protein ligase complex SCF(FBXL7) during mitosis, leading to its degradation by the proteasome By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiP59241.
    PRIDEiP59241.

    PTM databases

    PhosphoSiteiP59241.

    Expressioni

    Tissue specificityi

    Detected in neurons in brain cortex and hippocampus (at protein level). Expressed in mammary gland and tumor.1 Publication

    Inductioni

    Activated by progesterone.

    Gene expression databases

    GenevestigatoriP59241.

    Interactioni

    Subunit structurei

    Interacts with CPEB1, JTB, TACC1, TPX2, PPP2CA, as well as with the protein phosphatase type 1 (PP1) isoforms PPP1CA, PPP1CB and PPP1CC By similarity. Interacts also with its substrates ARHGEF2, BORA, BRCA1, KIF2A, PARD3, and p53/TP53. Interaction with BORA promotes phosphorylation of PLK1. Interacts with FBXL7 and PIFO. Interacts with GADD45A, competing with its oligomerization By similarity. Interacts (via C-terminus) with AUNIP (via C-terminus) By similarity. Identified in a complex with AUNIP and NIN By similarity. Interacts with FRY; this interaction facilitates AURKA-mediated PLK1 phosphorylation By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000051977.

    Structurei

    3D structure databases

    ProteinModelPortaliP59241.
    SMRiP59241. Positions 119-383.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini126 – 376251Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni273 – 28614Activation segmentBy similarityAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOVERGENiHBG108519.
    InParanoidiP59241.
    PhylomeDBiP59241.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P59241-1 [UniParc]FASTAAdd to Basket

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    MDRCKENCVS RPVKSTVPFG PKRVLVTEQI PSQHPGSASS GQAQRVLCPS    50
    NSQRVPPQAQ KPVAGQKPVL KQLPAASGPR PASRLSNPQK SEQPQPAASG 100
    NNSEKEQTSI QKTEDSKKRQ WTLEDFDIGR PLGKGKFGNV YLAREKQSKF 150
    ILALKVLFKV QLEKAGVEHQ LRREVEIQSH LRHPNILRLY GYFHDATRVY 200
    LILEYAPLGT VYRELQKLSK FDEQRTATYI TELANALSYC HSKRVIHRDI 250
    KPENLLLGSN GELKIADFGW SVHAPSSRRT TLCGTLDYQP PEMIEGRMHD 300
    EKVDLWSLGV LCYEFLVGMP PFEAHTYQET YRRISRVEFT FPDFVTEGAR 350
    DLISRLLKHN SSQRLTLAEV LEHPWIKANS SKPPTGHNSK EATSKSS 397
    Length:397
    Mass (Da):44,874
    Last modified:January 27, 2003 - v1
    Checksum:i95DECA2198DCED85
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF537333 mRNA. Translation: AAN06823.1.
    UniGeneiRn.161874.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF537333 mRNA. Translation: AAN06823.1 .
    UniGenei Rn.161874.

    3D structure databases

    ProteinModelPortali P59241.
    SMRi P59241. Positions 119-383.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000051977.

    PTM databases

    PhosphoSitei P59241.

    Proteomic databases

    PaxDbi P59241.
    PRIDEi P59241.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    RGDi 628895. Aurka.

    Phylogenomic databases

    eggNOGi COG0515.
    HOVERGENi HBG108519.
    InParanoidi P59241.
    PhylomeDBi P59241.

    Miscellaneous databases

    PROi P59241.

    Gene expression databases

    Genevestigatori P59241.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Centrosome amplification and overexpression of aurora A are early events in rat mammary carcinogenesis."
      Goepfert T.M., Adigun Y.E., Zhong L., Gay J., Medina D., Brinkley W.R.
      Cancer Res. 62:4115-4122(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar Furth.
      Tissue: Mammary gland.
    2. "Phosphorylation of the par polarity complex protein Par3 at serine 962 is mediated by aurora A and regulates its function in neuronal polarity."
      Khazaei M.R., Puschel A.W.
      J. Biol. Chem. 284:33571-33579(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiAURKA_RAT
    AccessioniPrimary (citable) accession number: P59241
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 27, 2003
    Last sequence update: January 27, 2003
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3