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Protein

Nephrocystin-4

Gene

Nphp4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the organization of apical junctions in kidney cells, together with NPHP1 and RPGRIP1L/NPHP8. Does not seem to be strictly required for ciliogenesis.1 Publication

GO - Biological processi

  • negative regulation of canonical Wnt signaling pathway Source: InterPro
  • photoreceptor cell maintenance Source: MGI
  • photoreceptor cell outer segment organization Source: MGI
  • retina development in camera-type eye Source: MGI
  • sperm motility Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-2028269. Signaling by Hippo.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Nephrocystin-4
Alternative name(s):
Nephroretinin
Gene namesi
Name:Nphp4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2384210. Nphp4.

Subcellular locationi

GO - Cellular componenti

  • bicellular tight junction Source: UniProtKB-SubCell
  • cell-cell junction Source: UniProtKB
  • centrosome Source: MGI
  • ciliary base Source: MGI
  • ciliary transition zone Source: MGI
  • cytoplasm Source: CACAO
  • photoreceptor connecting cilium Source: MGI
  • ribbon synapse Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell projection, Cilium, Cytoplasm, Cytoskeleton, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 14251425Nephrocystin-4PRO_0000159770Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei145 – 1451PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP59240.
PaxDbiP59240.
PeptideAtlasiP59240.
PRIDEiP59240.

PTM databases

iPTMnetiP59240.
PhosphoSiteiP59240.

Expressioni

Gene expression databases

BgeeiENSMUSG00000039577.
CleanExiMM_NPHP4.
GenevisibleiP59240. MM.

Interactioni

Subunit structurei

Interacts with NPHP1 and RPGRIP1L, and can bridge the interaction between those two proteins. Interacts with IQCB1; the interaction likely requires additional interactors. Weakly interacts with CEP164.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Nphp1Q9QY538EBI-4281265,EBI-77230
Rpgrip1lQ8CG734EBI-4281265,EBI-4281130

Protein-protein interaction databases

BioGridi234431. 213 interactions.
IntActiP59240. 67 interactions.
STRINGi10090.ENSMUSP00000049920.

Structurei

3D structure databases

ProteinModelPortaliP59240.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NPHP4 family.Curated

Phylogenomic databases

eggNOGiENOG410IEHW. Eukaryota.
ENOG410XW3A. LUCA.
GeneTreeiENSGT00510000048827.
HOGENOMiHOG000049142.
HOVERGENiHBG024036.
InParanoidiP59240.
KOiK16478.
OMAiFALKNPY.
OrthoDBiEOG091G0B0B.
TreeFamiTF351573.

Family and domain databases

InterProiIPR029775. NPHP4.
[Graphical view]
PANTHERiPTHR31043:SF3. PTHR31043:SF3. 1 hit.

Sequencei

Sequence statusi: Complete.

P59240-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGDWHRAFTQ NTLVPPHPQR ARQLGKESTA FQCILKWLDG PLIKQGILDM
60 70 80 90 100
LSELECHLRV TLFDVTYKHF FGRTWKTTVK PTNQPSKQPP RITFNEPLYF
110 120 130 140 150
HTTLSHPSIV AVVEVVTEGR KRDGTLQLLS CGFGILRIFG NKPESPTSAA
160 170 180 190 200
QDKRLRLYHG TPRALLHPLL QDPIEQNKFM RLMENCSLQY TLKPHPPLEP
210 220 230 240 250
AFHLLPENLL VSGFQQIPGL LPPHGDTGDA LRKPRFQKPT TWHLDDLFFT
260 270 280 290 300
LYPSLEKFEE ELVQLLISDR EGVGLLDSGT LEVLERRLHV CVHNGLGFVQ
310 320 330 340 350
RPQVVVLVPE MDVALTRSAS FSRKISASSK NSSGNQALVL RSHLRLPEMV
360 370 380 390 400
SHPAFAIVFQ LEYVFNSPSG ADGGASSPTS ISSVACMHMV RWAVWNPDLE
410 420 430 440 450
VGPGKVTLPL QGGVQQNPSR CLVYKVPSAS MSSEEVKQVE SGTIQFQFSL
460 470 480 490 500
SSDGPTEHAN GPRVGRRSSR KMPASPSGTP APAARDLAAT QDSPVGPGLS
510 520 530 540 550
LSQLTASPLS PALQSSSKPP LQPPDSSQSP EGPQLQAESV LESRVSHLEA
560 570 580 590 600
DLSQPASLQG TPAVEHLQEL PFTPLHAPIV VGAQTRSSRS QLSRAAMVLL
610 620 630 640 650
QSSGFPEILD ASQQPVEAVN PIDPVRFNPQ KEESDCLRGN EIVLQFLAFS
660 670 680 690 700
RAAQDCPGTP WPQTVYFTFQ FYRFPPETTP RLQLVKLDGT GKSGSGSLSH
710 720 730 740 750
ILVPINKDGS FDAGSPGLQL RYMVDPGFLK PGEQRWFAHY LAAQTLQVDV
760 770 780 790 800
WDGDSLLLIG SAGVQMKHLL RQGRPAVQVS HELEVVATEY EQEMMAVSGD
810 820 830 840 850
VAGFGSVKPI GVHTVVKGRL HLTLANVGHA CEPRARGSNL LPPSRSRVIS
860 870 880 890 900
NDGASFFSGG SLLIPGGPKR KRVVQAQRLA DVDSELAAML LTHTRAGQGP
910 920 930 940 950
QAAGQEADAV HKRKLERMRL VRLQEAGGDS DSRRISLLAQ HSVRAQHSRD
960 970 980 990 1000
LQVIDAYRER TKAESIAGVL SQAITTHHTL YATLGTAEFF EFALKNPHNT
1010 1020 1030 1040 1050
QHTVAIEIDS PELSIILDSQ EWRYFKEATG LHTPLEEDMF HLRGSLAPQL
1060 1070 1080 1090 1100
YLRPRETAHI PLKFQSFSVG PLAPTQAPAE VITEKDAESG PLWKCSAMPT
1110 1120 1130 1140 1150
KHAKVLFRVE TGQLIAVLCL TVEPQPHVVD QVFRFYHPEL TFLKKAIRLP
1160 1170 1180 1190 1200
PWHTLPGAPV GMPGEDPPVH VRCSDPNVIC EAQNVGPGEP RDVFLKVASG
1210 1220 1230 1240 1250
PSPEIKDFFV VIYADRWLAV PVQTWQVCLH SLQRVDVSCV AGQLTRLSLV
1260 1270 1280 1290 1300
LRGTQTVRKV RAFTSHPQEL KTDPAGVFVL PPHGVQDLHV GVRPRRAGSR
1310 1320 1330 1340 1350
FVHLNLVDID YHQLVASWLV CLSCRQPLIS KAFEITMAAG DEKGTNKRIT
1360 1370 1380 1390 1400
YTNPYPSRRT YRLHSDRPEL LRFKEDSFQV AGGETYTIGL RFLPSGSAGQ
1410 1420
EEILIYINDH EDKNEETFCV KVLYQ
Length:1,425
Mass (Da):157,269
Last modified:July 27, 2011 - v2
Checksum:iDDB9739850AEA5C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY118229 mRNA. Translation: AAM78559.1.
AL607109, AL611970 Genomic DNA. Translation: CAM21853.1.
AL611970, AL607109 Genomic DNA. Translation: CAM23668.1.
CH466594 Genomic DNA. Translation: EDL14939.1.
CH466594 Genomic DNA. Translation: EDL14940.1.
BC138370 mRNA. Translation: AAI38371.1.
CCDSiCCDS38985.1.
RefSeqiNP_700473.2. NM_153424.2.
XP_006538935.1. XM_006538872.2.
XP_006538936.1. XM_006538873.2.
UniGeneiMm.302496.

Genome annotation databases

EnsembliENSMUST00000056567; ENSMUSP00000049920; ENSMUSG00000039577.
ENSMUST00000081393; ENSMUSP00000080128; ENSMUSG00000039577.
GeneIDi260305.
KEGGimmu:260305.
UCSCiuc008wap.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY118229 mRNA. Translation: AAM78559.1.
AL607109, AL611970 Genomic DNA. Translation: CAM21853.1.
AL611970, AL607109 Genomic DNA. Translation: CAM23668.1.
CH466594 Genomic DNA. Translation: EDL14939.1.
CH466594 Genomic DNA. Translation: EDL14940.1.
BC138370 mRNA. Translation: AAI38371.1.
CCDSiCCDS38985.1.
RefSeqiNP_700473.2. NM_153424.2.
XP_006538935.1. XM_006538872.2.
XP_006538936.1. XM_006538873.2.
UniGeneiMm.302496.

3D structure databases

ProteinModelPortaliP59240.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi234431. 213 interactions.
IntActiP59240. 67 interactions.
STRINGi10090.ENSMUSP00000049920.

PTM databases

iPTMnetiP59240.
PhosphoSiteiP59240.

Proteomic databases

MaxQBiP59240.
PaxDbiP59240.
PeptideAtlasiP59240.
PRIDEiP59240.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000056567; ENSMUSP00000049920; ENSMUSG00000039577.
ENSMUST00000081393; ENSMUSP00000080128; ENSMUSG00000039577.
GeneIDi260305.
KEGGimmu:260305.
UCSCiuc008wap.1. mouse.

Organism-specific databases

CTDi261734.
MGIiMGI:2384210. Nphp4.

Phylogenomic databases

eggNOGiENOG410IEHW. Eukaryota.
ENOG410XW3A. LUCA.
GeneTreeiENSGT00510000048827.
HOGENOMiHOG000049142.
HOVERGENiHBG024036.
InParanoidiP59240.
KOiK16478.
OMAiFALKNPY.
OrthoDBiEOG091G0B0B.
TreeFamiTF351573.

Enzyme and pathway databases

ReactomeiR-MMU-2028269. Signaling by Hippo.
R-MMU-5620912. Anchoring of the basal body to the plasma membrane.

Miscellaneous databases

PROiP59240.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039577.
CleanExiMM_NPHP4.
GenevisibleiP59240. MM.

Family and domain databases

InterProiIPR029775. NPHP4.
[Graphical view]
PANTHERiPTHR31043:SF3. PTHR31043:SF3. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNPHP4_MOUSE
AccessioniPrimary (citable) accession number: P59240
Secondary accession number(s): B1AS30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 27, 2003
Last sequence update: July 27, 2011
Last modified: September 7, 2016
This is version 96 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.