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Protein

Ubiquitin-40S ribosomal protein S27a-2

Gene

RPS27AB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, and DNA-damage responses. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling (By similarity).By similarity
Ribosomal protein RSP27a-2 is a component of the 40S subunit of the ribosome.

Miscellaneous

Ubiquitin is encoded by 16 different genes. Ubiquitin is generally synthesized as a polyubiquitin precursor with tandem head to tail repeats. Often, there is one to three additional amino acids after the last repeat, removed in the mature protein. Alternatively, ubiquitin extension protein is synthesized as a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27A) or to a ubiquitin-related protein (either RUB1 or RUB2). Following translation, extension protein is cleaved from ubiquitin.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri121 – 144C4-typeAdd BLAST24

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • structural constituent of ribosome Source: CAFA

GO - Biological processi

  • translation Source: InterPro
  • ubiquitin-dependent protein catabolic process Source: TAIR

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-40S ribosomal protein S27a-2
Cleaved into the following 2 chains:
Gene namesi
Name:RPS27AB
Synonyms:UBQ6
Ordered Locus Names:At2g47110
ORF Names:F14M4.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G47110
TAIRilocus:2041459 AT2G47110

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003968721 – 76UbiquitinAdd BLAST76
ChainiPRO_000013767577 – 15740S ribosomal protein S27a-2Add BLAST81

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki76Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)PROSITE-ProRule annotation

Keywords - PTMi

Isopeptide bond

Proteomic databases

PaxDbiP59232
PRIDEiP59232

PTM databases

iPTMnetiP59232

Expressioni

Gene expression databases

ExpressionAtlasiP59232 baseline and differential
GenevisibleiP59232 AT

Interactioni

Subunit structurei

Ribosomal protein RSP27a-2 is part of the 40S ribosomal subunit.By similarity

Protein-protein interaction databases

BioGridi4658, 3 interactors
IntActiP59232, 2 interactors
STRINGi3702.AT2G47110.1

Structurei

3D structure databases

ProteinModelPortaliP59232
SMRiP59232
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 76Ubiquitin-likePROSITE-ProRule annotationAdd BLAST76

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi77 – 99Lys-rich (highly basic)Add BLAST23

Sequence similaritiesi

In the N-terminal section; belongs to the ubiquitin family.Curated
In the C-terminal section; belongs to the eukaryotic ribosomal protein eS31 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri121 – 144C4-typeAdd BLAST24

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0004 Eukaryota
COG1998 LUCA
COG5272 LUCA
InParanoidiP59232
KOiK02977
OMAiMSILKYY
OrthoDBiEOG09360S3F

Family and domain databases

Gene3Di2.20.25.660, 1 hit
InterProiView protein in InterPro
IPR002906 Ribosomal_S27a
IPR011332 Ribosomal_zn-bd
IPR038582 S27a-like_sf
IPR019956 Ubiquitin
IPR029071 Ubiquitin-like_domsf
IPR019954 Ubiquitin_CS
IPR000626 Ubiquitin_dom
PfamiView protein in Pfam
PF01599 Ribosomal_S27, 1 hit
PF00240 ubiquitin, 1 hit
PRINTSiPR00348 UBIQUITIN
SMARTiView protein in SMART
SM01402 Ribosomal_S27, 1 hit
SM00213 UBQ, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
SSF57829 SSF57829, 1 hit
PROSITEiView protein in PROSITE
PS00299 UBIQUITIN_1, 1 hit
PS50053 UBIQUITIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P59232-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIFVKTLTG KTITLEVESS DTIDNVKAKI QDKEGIPPDQ QRLIFAGKQL
60 70 80 90 100
EDGRTLADYN IQKESTLHLV LRLRGGAKKR KKKTYTKPKK IKHKHKKVKL
110 120 130 140 150
AVLQFYKVDG SGKVQRLRKE CPNATCGAGT FMASHFDRHY CGKCGLTYVY

QKEGAQE
Length:157
Mass (Da):17,812
Last modified:August 10, 2010 - v2
Checksum:iAE3B7797763FD558
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05540 Genomic DNA Translation: AAA32907.1
AC004411 Genomic DNA Translation: AAC34235.1
CP002685 Genomic DNA Translation: AEC10801.1
CP002685 Genomic DNA Translation: AEC10802.1
AF375416 mRNA Translation: AAK53000.1
AY142033 mRNA Translation: AAM98297.1
AY143810 mRNA Translation: AAN28749.1
AY088370 mRNA Translation: AAM65909.1
PIRiD36571
RefSeqiNP_001189768.1, NM_001202839.1
NP_566095.1, NM_130278.3
UniGeneiAt.27680
At.75284

Genome annotation databases

EnsemblPlantsiAT2G47110.1; AT2G47110.1; AT2G47110
AT2G47110.2; AT2G47110.2; AT2G47110
GeneIDi819323
GrameneiAT2G47110.1; AT2G47110.1; AT2G47110
AT2G47110.2; AT2G47110.2; AT2G47110
KEGGiath:AT2G47110

Similar proteinsi

Entry informationi

Entry nameiR27AB_ARATH
AccessioniPrimary (citable) accession number: P59232
Secondary accession number(s): O80715
, P19233, P59263, Q38875, Q9LDJ2, Q9LYW1, Q9M0W3, Q9M1P9, Q9S7X3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: August 10, 2010
Last modified: April 25, 2018
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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