Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Guanine nucleotide-binding protein G(o) subunit alpha

Gene

Gnao1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(o) protein function is not clear. Stimulated by RGS14 (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi47MagnesiumBy similarity1
Metal bindingi182MagnesiumBy similarity1
Binding sitei326GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi40 – 47GTPBy similarity8
Nucleotide bindingi176 – 182GTPBy similarity7
Nucleotide bindingi201 – 205GTPBy similarity5
Nucleotide bindingi270 – 273GTPBy similarity4

GO - Molecular functioni

  • corticotropin-releasing hormone receptor 1 binding Source: RGD
  • GDP binding Source: RGD
  • G-protein beta/gamma-subunit complex binding Source: GO_Central
  • G-protein coupled serotonin receptor binding Source: RGD
  • GTPase activating protein binding Source: RGD
  • GTPase activity Source: RGD
  • GTP binding Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • mu-type opioid receptor binding Source: RGD
  • protein complex binding Source: RGD
  • signal transducer activity Source: GO_Central

GO - Biological processi

  • adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: GO_Central
  • aging Source: RGD
  • dopamine receptor signaling pathway Source: GO_Central
  • forebrain development Source: RGD
  • G-protein coupled receptor signaling pathway Source: RGD
  • locomotory behavior Source: Ensembl
  • negative regulation of calcium ion transport Source: RGD
  • neuron projection development Source: RGD
  • positive regulation of GTPase activity Source: RGD
  • regulation of heart contraction Source: Ensembl
  • response to cytokine Source: RGD
  • response to drug Source: RGD
  • response to hydrogen peroxide Source: RGD
  • response to morphine Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to organonitrogen compound Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-112043. PLC beta mediated events.
R-RNO-202040. G-protein activation.
R-RNO-4086398. Ca2+ pathway.
R-RNO-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(o) subunit alpha
Gene namesi
Name:Gnao1
Synonyms:Gna0, Gnao
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi628732. Gnao1.

Subcellular locationi

  • Cell membrane By similarity
  • Membrane By similarity; Lipid-anchor By similarity

GO - Cellular componenti

  • cell body Source: Ensembl
  • dendrite Source: Ensembl
  • heterotrimeric G-protein complex Source: GO_Central
  • membrane Source: RGD
  • myelin sheath Source: UniProtKB
  • neuron projection Source: RGD
  • protein complex Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002037062 – 354Guanine nucleotide-binding protein G(o) subunit alphaAdd BLAST353

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine1 Publication1
Lipidationi3S-palmitoyl cysteine1 Publication1
Modified residuei346Deamidated asparagine; in form Alpha-31 Publication1

Post-translational modificationi

Deamidation of Asn-346 converts alpha-1 to alpha-3.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei351Not S-palmitoylated1

Keywords - PTMi

Lipoprotein, Myristate, Palmitate

Proteomic databases

PaxDbiP59215.
PRIDEiP59215.

PTM databases

iPTMnetiP59215.
PhosphoSitePlusiP59215.
SwissPalmiP59215.

Expressioni

Gene expression databases

BgeeiENSRNOG00000019482.
GenevisibleiP59215. RN.

Interactioni

Subunit structurei

Interacts with RGS14 (By similarity). G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.By similarity

GO - Molecular functioni

  • corticotropin-releasing hormone receptor 1 binding Source: RGD
  • G-protein beta/gamma-subunit complex binding Source: GO_Central
  • G-protein coupled serotonin receptor binding Source: RGD
  • GTPase activating protein binding Source: RGD
  • mu-type opioid receptor binding Source: RGD
  • protein complex binding Source: RGD

Protein-protein interaction databases

BioGridi248412. 3 interactors.
DIPiDIP-59090N.
IntActiP59215. 5 interactors.
MINTiMINT-5028451.
STRINGi10116.ENSRNOP00000026373.

Structurei

3D structure databases

ProteinModelPortaliP59215.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

Phylogenomic databases

eggNOGiKOG0082. Eukaryota.
ENOG410XNVQ. LUCA.
GeneTreeiENSGT00760000118851.
HOGENOMiHOG000038730.
HOVERGENiHBG063184.
InParanoidiP59215.
KOiK04534.
OMAiDSKMVCD.
OrthoDBiEOG091G0VUT.
PhylomeDBiP59215.
TreeFamiTF300673.

Family and domain databases

CDDicd00066. G-alpha. 1 hit.
Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Alpha-1 (identifier: P59215-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGCTLSAEER AALERSKAIE KNLKEDGISA AKDVKLLLLG AGESGKSTIV
60 70 80 90 100
KQMKIIHEDG FSGEDVKQYK PVVYSNTIQS LAAIVRAMDT LGVEYGDKER
110 120 130 140 150
KADSKMVCDV VSRMEDTEPF SAELLSAMMR LWGDSGIQEC FNRSREYQLN
160 170 180 190 200
DSAKYYLDSL DRIGAADYQP TEQDILRTRV KTTGIVETHF TFKNLHFRLF
210 220 230 240 250
DVGGQRSERK KWIHCFEDVT AIIFCVALSG YDQVLHEDET TNRMHESLML
260 270 280 290 300
FDSICNNKFF IDTSIILFLN KKDLFGEKIK KSPLTICFPE YPGSNTYEDA
310 320 330 340 350
AAYIQTQFES KNRSPNKEIY CHMTCATDTN NIQVVFDAVT DIIIANNLRG

CGLY
Length:354
Mass (Da):40,069
Last modified:January 23, 2007 - v2
Checksum:i577024F61B179C89
GO
Isoform Alpha-2 (identifier: P59215-2) [UniParc] [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     247-354: SLMLFDSICN...ANNLRGCGLY → FLKLFDSICN...AKNLRGCGLY

Show »
Length:354
Mass (Da):40,081
Checksum:i1C9D8AB39DC03CE3
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_031252247 – 354SLMLF…GCGLY → FLKLFDSICNNKWFTDTSII LFLNKKDIFEEKIKKSPLTI CFPEYTGPSAFTEAVAHIQG QYESKNKSAHKEVYSHVTCA TDTNNIQFVFDAVTDVIIAK NLRGCGLY in isoform Alpha-2. 1 PublicationAdd BLAST108

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17526 mRNA. Translation: AAA40826.1.
M12671 mRNA. Translation: AAA41262.1.
PIRiC40436. RGRTO1.
D40436. RGRTO2.
RefSeqiNP_059023.1. NM_017327.1. [P59215-1]
UniGeneiRn.90161.

Genome annotation databases

EnsembliENSRNOT00000026373; ENSRNOP00000026373; ENSRNOG00000019482. [P59215-1]
GeneIDi50664.
KEGGirno:50664.
UCSCiRGD:628732. rat. [P59215-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17526 mRNA. Translation: AAA40826.1.
M12671 mRNA. Translation: AAA41262.1.
PIRiC40436. RGRTO1.
D40436. RGRTO2.
RefSeqiNP_059023.1. NM_017327.1. [P59215-1]
UniGeneiRn.90161.

3D structure databases

ProteinModelPortaliP59215.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248412. 3 interactors.
DIPiDIP-59090N.
IntActiP59215. 5 interactors.
MINTiMINT-5028451.
STRINGi10116.ENSRNOP00000026373.

PTM databases

iPTMnetiP59215.
PhosphoSitePlusiP59215.
SwissPalmiP59215.

Proteomic databases

PaxDbiP59215.
PRIDEiP59215.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000026373; ENSRNOP00000026373; ENSRNOG00000019482. [P59215-1]
GeneIDi50664.
KEGGirno:50664.
UCSCiRGD:628732. rat. [P59215-1]

Organism-specific databases

CTDi2775.
RGDi628732. Gnao1.

Phylogenomic databases

eggNOGiKOG0082. Eukaryota.
ENOG410XNVQ. LUCA.
GeneTreeiENSGT00760000118851.
HOGENOMiHOG000038730.
HOVERGENiHBG063184.
InParanoidiP59215.
KOiK04534.
OMAiDSKMVCD.
OrthoDBiEOG091G0VUT.
PhylomeDBiP59215.
TreeFamiTF300673.

Enzyme and pathway databases

ReactomeiR-RNO-112043. PLC beta mediated events.
R-RNO-202040. G-protein activation.
R-RNO-4086398. Ca2+ pathway.
R-RNO-6814122. Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.

Miscellaneous databases

PROiP59215.

Gene expression databases

BgeeiENSRNOG00000019482.
GenevisibleiP59215. RN.

Family and domain databases

CDDicd00066. G-alpha. 1 hit.
Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiGNAO_RAT
AccessioniPrimary (citable) accession number: P59215
Secondary accession number(s): P04900, P30033
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 1987
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.