Reviewed, UniProtKB/Swiss-Prot P59206 (LYTB_STRR6)

Names and origin

Protein names

Recommended name:
    Putative endo-beta-N-acetylglucosaminidase
    EC=3.2.1.96
Alternative name(s):
    Murein hydrolase

Gene names

Name:	lytB
Ordered Locus Names:	spr0867

Organism

Streptococcus pneumoniae (strain ATCC BAA-255 / R6) [Complete proteome] [HAMAP]

Taxonomic identifier

171101 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	702 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays an important role in cell wall degradation and cell separation.
Catalytic activity	Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)2)Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 73 family. Contains 15 cell wall-binding repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall macromolecule metabolic process Inferred from electronic annotation. Source: InterPro peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amidase activity Inferred from electronic annotation. Source: InterPro mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Ref.1
	Chain	24 – 702	679	Putative endo-beta-N-acetylglucosaminidase		PRO_0000012117
Regions
	Repeat	42 – 63	22	Cell wall-binding 1
	Repeat	65 – 84	20	Cell wall-binding 2
	Repeat	86 – 105	20	Cell wall-binding 3
	Repeat	124 – 145	22	Cell wall-binding 4
	Repeat	147 – 166	20	Cell wall-binding 5
	Repeat	185 – 206	22	Cell wall-binding 6
	Repeat	208 – 227	20	Cell wall-binding 7
	Repeat	229 – 248	20	Cell wall-binding 8
	Repeat	250 – 271	22	Cell wall-binding 9
	Repeat	273 – 292	20	Cell wall-binding 10
	Repeat	294 – 315	22	Cell wall-binding 11
	Repeat	317 – 336	20	Cell wall-binding 12
	Repeat	338 – 359	22	Cell wall-binding 13
	Repeat	361 – 380	20	Cell wall-binding 14
	Repeat	382 – 403	22	Cell wall-binding 15

Sequences

	Sequence		Length	Mass (Da)
	P59206-1 [UniParc]. Last modified January 10, 2003. Version 1. Checksum: 84B6536E75301FE5	FASTA	702	81,900
	10 20 30 40 50 60 MKKVRFIFLA LLFFLASPEG AMASDGTWQG KQYLKEDGSQ AANEWVFDTH YQSWFYIKAD 70 80 90 100 110 120 ANYAENEWLK QGDDYFYLKS GGYMAKSEWV EDKGAFYYLD QDGKMKRNAW VGTSYVGATG 130 140 150 160 170 180 AKVIEDWVYD SQYDAWFYIK ADGQHAEKEW LQIKGKDYYF KSGGYLLTSQ WINQAYVNAS 190 200 210 220 230 240 GAKVQQGWLF DKQYQSWFYI KENGNYADKE WIFENGHYYY LKSGGYMAAN EWIWDKESWF 250 260 270 280 290 300 YLKFDGKIAE KEWVYDSHSQ AWYYFKSGGY MAANEWIWDK ESWFYLKFDG KMAEKEWVYD 310 320 330 340 350 360 SHSQAWYYFK SGGYMTANEW IWDKESWFYL KSDGKIAEKE WVYDSHSQAW YYFKSGGYMT 370 380 390 400 410 420 ANEWIWDKES WFYLKSDGKM AEKEWVYDSH SQAWYYFKSG GYMAKNETVD GYQLGSDGKW 430 440 450 460 470 480 LGGKATNKNA AYYQVVPVTA NVYDSDGEKL SYISQGSVVW LDKDRKSDDK RLAITISGLS 490 500 510 520 530 540 GYMKTEDLQA LDASKDFIPY YESDGHRFYH YVAQNASIPV ASHLSDMEVG KKYYSADGLH 550 560 570 580 590 600 FDGFKLENPF LFKDLTEATN YSAEELDKVF SLLNINNSLL ENKGATFKEA EEHYHINALY 610 620 630 640 650 660 LLAHSALESN WGRSKIAKDK NNFFGITAYD TTPYLSAKTF DDVDKGILGA TKWIKENYID 670 680 690 700 RGRTFLGNKA SGMNVEYASD PYWGEKIASV MMKINEKLGG KD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"LytB, a novel pneumococcal murein hydrolase essential for cell separation." Garcia P., Gonzalez M.P., Garcia E., Lopez R., Garcia J.L. Mol. Microbiol. 31:1275-1281(1999) [PubMed: 10096093] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 24-30.
[2]	Garcia J. Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Genome of the bacterium Streptococcus pneumoniae strain R6." Hoskins J., Alborn W.E. Jr., Arnold J., Blaszczak L.C., Burgett S., DeHoff B.S., Estrem S.T., Fritz L., Fu D.-J., Fuller W., Geringer C., Gilmour R., Glass J.S., Khoja H., Kraft A.R., Lagace R.E., LeBlanc D.J., Lee L.N. , Lefkowitz E.J., Lu J., Matsushima P., McAhren S.M., McHenney M., McLeaster K., Mundy C.W., Nicas T.I., Norris F.H., O'Gara M., Peery R.B., Robertson G.T., Rockey P., Sun P.-M., Winkler M.E., Yang Y., Young-Bellido M., Zhao G., Zook C.A., Baltz R.H., Jaskunas S.R., Rosteck P.R. Jr., Skatrud P.L., Glass J.I. J. Bacteriol. 183:5709-5717(2001) [PubMed: 11544234] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ010312 Genomic DNA. Translation: CAA09078.2. AE007317 Genomic DNA. Translation: AAK99671.1. Different initiation.
PIR	C97980.
RefSeq	NP_358461.1.
3D structure databases
HSSP	HSSP built from PDB template 1GVM based on UniProtKB P06653.
ModBase	Search...
Protein-protein interaction databases
STRING	P59206.
Protein family/group databases
CAZy	GH73. Glycoside Hydrolase Family 73.
Genome annotation databases
GeneID	934406.
GenomeReviews	Gene locus spr0867 in contig AE007317_GR.
KEGG	spr:spr0867.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P59206.
Enzyme and pathway databases
BioCyc	SPNE171101:SPR0867-MON.
Family and domain databases
InterPro	IPR018337. Cell_wall/Cho-bd_repeat. IPR002479. Cell_wall_bd_put. IPR013338. Lyz2. IPR002901. Mano_Glyc_endo_b_GlcNAc. [Graphical view]
Pfam	PF01473. CW_binding_1. 12 hits. PF01832. Glucosaminidase. 1 hit. [Graphical view]
SMART	SM00047. LYZ2. 1 hit. [Graphical view]
PROSITE	PS51170. CW. 15 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LYTB_STRR6

Accession

Primary (citable) accession number: P59206
Secondary accession number(s): Q9Z4P7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2003
Last sequence update:	January 10, 2003
Last modified:	September 1, 2009
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)