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P59158 (S12A3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Solute carrier family 12 member 3
Alternative name(s):
Na-Cl symporter
Thiazide-sensitive sodium-chloride cotransporter
Gene names
Name:Slc12a3
Synonyms:Tsc
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length1002 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Electrically silent transporter system. Mediates sodium and chloride reabsorption By similarity.

Enzyme regulation

Activated by WNK3 By similarity.

Subunit structure

Interacts with KLHL3 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Post-translational modification

Ubiquitinated; ubiquitination is essential for regulation of endocytosis. May be ubiquitinated by the BCR(KLHL3) complex; leading to regulate subcellular location at the cell surface. Ref.2

Sequence similarities

Belongs to the SLC12A transporter family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10021002Solute carrier family 12 member 3
PRO_0000178027

Regions

Topological domain1 – 133133Cytoplasmic Potential
Transmembrane134 – 15421Helical; Potential
Transmembrane165 – 18521Helical; Potential
Topological domain186 – 21631Cytoplasmic Potential
Transmembrane217 – 23721Helical; Potential
Transmembrane260 – 28021Helical; Potential
Topological domain281 – 2844Cytoplasmic Potential
Transmembrane285 – 30521Helical; Potential
Transmembrane338 – 35821Helical; Potential
Topological domain359 – 37517Cytoplasmic Potential
Transmembrane376 – 39621Helical; Potential
Transmembrane451 – 47121Helical; Potential
Topological domain472 – 50938Cytoplasmic Potential
Transmembrane510 – 53021Helical; Potential
Transmembrane576 – 59621Helical; Potential
Topological domain597 – 65862Cytoplasmic Potential
Transmembrane659 – 67921Helical; Potential
Transmembrane811 – 83121Helical; Potential
Topological domain832 – 1002171Cytoplasmic Potential

Amino acid modifications

Glycosylation4041N-linked (GlcNAc...) Potential
Glycosylation4241N-linked (GlcNAc...) Potential
Glycosylation5421N-linked (GlcNAc...) Potential
Glycosylation7791N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P59158 [UniParc].

Last modified December 13, 2002. Version 1.
Checksum: 61CB02500C9093B9

FASTA1,002110,694
        10         20         30         40         50         60 
MAELPVTELP GDALCSGRFT ISTLMGGDEP PPAACDSSQP SHLTHGSTLY MRTFGYNTID 

        70         80         90        100        110        120 
VVPAYEHYAN SALPGEPRKV RPTLADLHSF LKQEGSHLHA LAFDGRQGRE LTDGLVEDET 

       130        140        150        160        170        180 
GTNSEKSPGE PVRFGWVKGV MIRCMLNIWG VILYLRLPWI TAQAGIVLTW LIILLSVMVT 

       190        200        210        220        230        240 
SITGLSISAI STNGKVKSGG TYFLISRSLG PELGGSIGLI FAFANAVGVA MHTVGFAETV 

       250        260        270        280        290        300 
RDLLQEYGTP IVDPINDIRI IGVVTVTVLL AISLAGMEWE SKAQVLFFLV IMVSFANYLV 

       310        320        330        340        350        360 
GTLIPASEDK ASKGFYSYHG DIFVQNLVPD WRGIDGSFFG MFSIFFPSAT GILAGANISG 

       370        380        390        400        410        420 
DLKDPAVAIP KGTLMAIFWT TISYLAISAT IGSCVVRDAS GDVNDTMTPG PGPCEGLACG 

       430        440        450        460        470        480 
YGWNFTECSQ QRSCRYGLIN YYQTMSMVSA FAPLITAGIF GATLSSALAC LVSAAKVFQC 

       490        500        510        520        530        540 
LCEDQLYPLI GFFGKGYGKN REPVRGYLLA YAIAVAFIII AELNTIAPII SNFFLCSYAL 

       550        560        570        580        590        600 
INFSCFHASI TNSPGWRPSF RYYSKWAALF GAVISVVIMF LLTWWAALIA IGVVLFLLLY 

       610        620        630        640        650        660 
VIYKKPEVNW GSSVQAGSYN LALSYSVGLN EVEDHIKNYR PQCLVLTGPP NFRPALVDFV 

       670        680        690        700        710        720 
STFTQNLSLM ICGHVLIGPG KQRVPELRLI ASGHTKWLNK RKIKAFYSDV IAEDLRSGVQ 

       730        740        750        760        770        780 
ILMQASGLGR MKPNILVVGF KRNWQSAHPA TVEDYIGVLH DAFDFNYGVC VMRMREGLNV 

       790        800        810        820        830        840 
SEALQTHTTP EALIQEEQAS TIFQSEQGKK TIDIYWLFDD GGLTLLIPYL LHRKKRWGKC 

       850        860        870        880        890        900 
KIRVFVGGQI NRMDEERKAI ISLLSKFRLG FHEVHVLPDI NQKPQAEHTK RFEDMIAPFR 

       910        920        930        940        950        960 
LNDGFKDEAT VTEMRRDCPW KISDEEINKN RIKSLRQVRL SEILLDYSRD AALIILTLPI 

       970        980        990       1000 
GRKGKCPSSL YMAWLETLSQ DLRPPVLLIR GNQENVLTFY CQ

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Kidney.
[2]"RasGRP1 stimulation enhances ubiquitination and endocytosis of the sodium-chloride cotransporter."
Ko B., Kamsteeg E.J., Cooke L.L., Moddes L.N., Deen P.M., Hoover R.S.
Am. J. Physiol. 299:F300-F309(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BC038612 mRNA. Translation: AAH38612.1.
IPIIPI00173140.
RefSeqNP_001192240.1. NM_001205311.1.
UniGeneMm.25804.

3D structure databases

ProteinModelPortalP59158.
ModBaseSearch...

PTM databases

PhosphoSiteP59158.

Proteomic databases

PaxDbP59158.
PRIDEP59158.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034218; ENSMUSP00000034218; ENSMUSG00000031766.
GeneID20497.
KEGGmmu:20497.

Organism-specific databases

CTD6559.
MGIMGI:108114. Slc12a3.

Phylogenomic databases

eggNOGCOG0531.
HOGENOMHOG000062855.
HOVERGENHBG052851.
InParanoidP59158.
KOK14426.
OMAIGRKGKC.
OrthoDBEOG4CC40K.

Gene expression databases

ArrayExpressP59158.
BgeeP59158.
CleanExMM_SLC12A3.
GenevestigatorP59158.
GermOnlineENSMUSG00000031766. Mus musculus.

Family and domain databases

InterProIPR004841. AA-permease_dom.
IPR013612. AA_permease_N.
IPR004842. Na/K/Cl_cotransptS.
IPR002948. NaCl_cotranspt.
[Graphical view]
PfamPF00324. AA_permease. 1 hit.
PF08403. AA_permease_N. 1 hit.
[Graphical view]
PRINTSPR01230. NACLTRNSPORT.
TIGRFAMsTIGR00930. 2a30. 1 hit.
ProtoNetSearch...

Other

ChiTaRSSLC12A3. mouse.
NextBio298655.
SOURCESearch...

Entry information

Entry nameS12A3_MOUSE
AccessionPrimary (citable) accession number: P59158
Entry history
Integrated into UniProtKB/Swiss-Prot: December 13, 2002
Last sequence update: December 13, 2002
Last modified: April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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