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P59158

- S12A3_MOUSE

UniProt

P59158 - S12A3_MOUSE

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Protein

Solute carrier family 12 member 3

Gene
Slc12a3, Ncc, Tsc
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Electrically silent transporter system. Mediates sodium and chloride reabsorption By similarity.

Enzyme regulationi

Activated by WNK3 By similarity.

GO - Molecular functioni

  1. cation:chloride symporter activity Source: InterPro
  2. transporter activity Source: UniProtKB

GO - Biological processi

  1. sodium ion transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 12 member 3
Alternative name(s):
Na-Cl symporter
Thiazide-sensitive sodium-chloride cotransporter
Gene namesi
Name:Slc12a3
Synonyms:Ncc, Tsc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:108114. Slc12a3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 133133Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei134 – 15421Helical; Reviewed predictionAdd
BLAST
Transmembranei165 – 18521Helical; Reviewed predictionAdd
BLAST
Topological domaini186 – 21631Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei217 – 23721Helical; Reviewed predictionAdd
BLAST
Transmembranei260 – 28021Helical; Reviewed predictionAdd
BLAST
Topological domaini281 – 2844Cytoplasmic Reviewed prediction
Transmembranei285 – 30521Helical; Reviewed predictionAdd
BLAST
Transmembranei338 – 35821Helical; Reviewed predictionAdd
BLAST
Topological domaini359 – 37517Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei376 – 39621Helical; Reviewed predictionAdd
BLAST
Transmembranei451 – 47121Helical; Reviewed predictionAdd
BLAST
Topological domaini472 – 50938Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei510 – 53021Helical; Reviewed predictionAdd
BLAST
Transmembranei576 – 59621Helical; Reviewed predictionAdd
BLAST
Topological domaini597 – 65862Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei659 – 67921Helical; Reviewed predictionAdd
BLAST
Transmembranei811 – 83121Helical; Reviewed predictionAdd
BLAST
Topological domaini832 – 1002171Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB
  2. cytosol Source: UniProtKB
  3. integral component of membrane Source: UniProtKB-KW
  4. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10021002Solute carrier family 12 member 3PRO_0000178027Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi404 – 4041N-linked (GlcNAc...) Reviewed prediction
Glycosylationi424 – 4241N-linked (GlcNAc...) Reviewed prediction
Glycosylationi542 – 5421N-linked (GlcNAc...) Reviewed prediction
Glycosylationi779 – 7791N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

Ubiquitinated; ubiquitination is essential for regulation of endocytosis.1 Publication

Keywords - PTMi

Glycoprotein, Ubl conjugation

Proteomic databases

PaxDbiP59158.
PRIDEiP59158.

PTM databases

PhosphoSiteiP59158.

Expressioni

Gene expression databases

ArrayExpressiP59158.
BgeeiP59158.
CleanExiMM_SLC12A3.
GenevestigatoriP59158.

Interactioni

Subunit structurei

Interacts with KLHL3 By similarity.

Protein-protein interaction databases

BioGridi203278. 1 interaction.
IntActiP59158. 1 interaction.
MINTiMINT-4133700.

Structurei

3D structure databases

ProteinModelPortaliP59158.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0531.
HOGENOMiHOG000062855.
HOVERGENiHBG052851.
InParanoidiP59158.
KOiK14426.
OMAiQLYPLIG.
OrthoDBiEOG75MVVC.
PhylomeDBiP59158.
TreeFamiTF313191.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR013612. AA_permease_N.
IPR004842. Na/K/Cl_cotransptS.
IPR002948. NaCl_cotranspt.
[Graphical view]
PfamiPF00324. AA_permease. 1 hit.
PF08403. AA_permease_N. 1 hit.
[Graphical view]
PRINTSiPR01230. NACLTRNSPORT.
TIGRFAMsiTIGR00930. 2a30. 1 hit.

Sequencei

Sequence statusi: Complete.

P59158-1 [UniParc]FASTAAdd to Basket

« Hide

MAELPVTELP GDALCSGRFT ISTLMGGDEP PPAACDSSQP SHLTHGSTLY     50
MRTFGYNTID VVPAYEHYAN SALPGEPRKV RPTLADLHSF LKQEGSHLHA 100
LAFDGRQGRE LTDGLVEDET GTNSEKSPGE PVRFGWVKGV MIRCMLNIWG 150
VILYLRLPWI TAQAGIVLTW LIILLSVMVT SITGLSISAI STNGKVKSGG 200
TYFLISRSLG PELGGSIGLI FAFANAVGVA MHTVGFAETV RDLLQEYGTP 250
IVDPINDIRI IGVVTVTVLL AISLAGMEWE SKAQVLFFLV IMVSFANYLV 300
GTLIPASEDK ASKGFYSYHG DIFVQNLVPD WRGIDGSFFG MFSIFFPSAT 350
GILAGANISG DLKDPAVAIP KGTLMAIFWT TISYLAISAT IGSCVVRDAS 400
GDVNDTMTPG PGPCEGLACG YGWNFTECSQ QRSCRYGLIN YYQTMSMVSA 450
FAPLITAGIF GATLSSALAC LVSAAKVFQC LCEDQLYPLI GFFGKGYGKN 500
REPVRGYLLA YAIAVAFIII AELNTIAPII SNFFLCSYAL INFSCFHASI 550
TNSPGWRPSF RYYSKWAALF GAVISVVIMF LLTWWAALIA IGVVLFLLLY 600
VIYKKPEVNW GSSVQAGSYN LALSYSVGLN EVEDHIKNYR PQCLVLTGPP 650
NFRPALVDFV STFTQNLSLM ICGHVLIGPG KQRVPELRLI ASGHTKWLNK 700
RKIKAFYSDV IAEDLRSGVQ ILMQASGLGR MKPNILVVGF KRNWQSAHPA 750
TVEDYIGVLH DAFDFNYGVC VMRMREGLNV SEALQTHTTP EALIQEEQAS 800
TIFQSEQGKK TIDIYWLFDD GGLTLLIPYL LHRKKRWGKC KIRVFVGGQI 850
NRMDEERKAI ISLLSKFRLG FHEVHVLPDI NQKPQAEHTK RFEDMIAPFR 900
LNDGFKDEAT VTEMRRDCPW KISDEEINKN RIKSLRQVRL SEILLDYSRD 950
AALIILTLPI GRKGKCPSSL YMAWLETLSQ DLRPPVLLIR GNQENVLTFY 1000
CQ 1002
Length:1,002
Mass (Da):110,694
Last modified:December 13, 2002 - v1
Checksum:i61CB02500C9093B9
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC038612 mRNA. Translation: AAH38612.1.
CCDSiCCDS57632.1.
RefSeqiNP_001192240.1. NM_001205311.1.
UniGeneiMm.25804.

Genome annotation databases

EnsembliENSMUST00000034218; ENSMUSP00000034218; ENSMUSG00000031766.
GeneIDi20497.
KEGGimmu:20497.
UCSCiuc009mwc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC038612 mRNA. Translation: AAH38612.1 .
CCDSi CCDS57632.1.
RefSeqi NP_001192240.1. NM_001205311.1.
UniGenei Mm.25804.

3D structure databases

ProteinModelPortali P59158.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 203278. 1 interaction.
IntActi P59158. 1 interaction.
MINTi MINT-4133700.

PTM databases

PhosphoSitei P59158.

Proteomic databases

PaxDbi P59158.
PRIDEi P59158.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000034218 ; ENSMUSP00000034218 ; ENSMUSG00000031766 .
GeneIDi 20497.
KEGGi mmu:20497.
UCSCi uc009mwc.2. mouse.

Organism-specific databases

CTDi 6559.
MGIi MGI:108114. Slc12a3.

Phylogenomic databases

eggNOGi COG0531.
HOGENOMi HOG000062855.
HOVERGENi HBG052851.
InParanoidi P59158.
KOi K14426.
OMAi QLYPLIG.
OrthoDBi EOG75MVVC.
PhylomeDBi P59158.
TreeFami TF313191.

Miscellaneous databases

ChiTaRSi SLC12A3. mouse.
NextBioi 298655.
PROi P59158.
SOURCEi Search...

Gene expression databases

ArrayExpressi P59158.
Bgeei P59158.
CleanExi MM_SLC12A3.
Genevestigatori P59158.

Family and domain databases

InterProi IPR004841. AA-permease/SLC12A_dom.
IPR013612. AA_permease_N.
IPR004842. Na/K/Cl_cotransptS.
IPR002948. NaCl_cotranspt.
[Graphical view ]
Pfami PF00324. AA_permease. 1 hit.
PF08403. AA_permease_N. 1 hit.
[Graphical view ]
PRINTSi PR01230. NACLTRNSPORT.
TIGRFAMsi TIGR00930. 2a30. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.
  2. "RasGRP1 stimulation enhances ubiquitination and endocytosis of the sodium-chloride cotransporter."
    Ko B., Kamsteeg E.J., Cooke L.L., Moddes L.N., Deen P.M., Hoover R.S.
    Am. J. Physiol. 299:F300-F309(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION.

Entry informationi

Entry nameiS12A3_MOUSE
AccessioniPrimary (citable) accession number: P59158
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 13, 2002
Last sequence update: December 13, 2002
Last modified: July 9, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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