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P59158

- S12A3_MOUSE

UniProt

P59158 - S12A3_MOUSE

Protein

Solute carrier family 12 member 3

Gene

Slc12a3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (13 Dec 2002)
      Previous versions | rss
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    Functioni

    Electrically silent transporter system. Mediates sodium and chloride reabsorption By similarity.By similarity

    Enzyme regulationi

    Activated by WNK3.By similarity

    GO - Molecular functioni

    1. cation:chloride symporter activity Source: InterPro
    2. transporter activity Source: UniProtKB

    GO - Biological processi

    1. sodium ion transport Source: UniProtKB

    Keywords - Biological processi

    Ion transport, Sodium transport, Symport, Transport

    Keywords - Ligandi

    Sodium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Solute carrier family 12 member 3
    Alternative name(s):
    Na-Cl symporter
    Thiazide-sensitive sodium-chloride cotransporter
    Gene namesi
    Name:Slc12a3
    Synonyms:Ncc, Tsc
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:108114. Slc12a3.

    Subcellular locationi

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB
    2. cytosol Source: UniProtKB
    3. integral component of membrane Source: UniProtKB-KW
    4. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10021002Solute carrier family 12 member 3PRO_0000178027Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi404 – 4041N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi424 – 4241N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi542 – 5421N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi779 – 7791N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Ubiquitinated; ubiquitination is essential for regulation of endocytosis.1 Publication

    Keywords - PTMi

    Glycoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiP59158.
    PRIDEiP59158.

    PTM databases

    PhosphoSiteiP59158.

    Expressioni

    Gene expression databases

    ArrayExpressiP59158.
    BgeeiP59158.
    CleanExiMM_SLC12A3.
    GenevestigatoriP59158.

    Interactioni

    Subunit structurei

    Interacts with KLHL3.By similarity

    Protein-protein interaction databases

    BioGridi203278. 1 interaction.
    IntActiP59158. 1 interaction.
    MINTiMINT-4133700.

    Structurei

    3D structure databases

    ProteinModelPortaliP59158.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 133133CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini186 – 21631CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini281 – 2844CytoplasmicSequence Analysis
    Topological domaini359 – 37517CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini472 – 50938CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini597 – 65862CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini832 – 1002171CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei134 – 15421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei165 – 18521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei217 – 23721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei260 – 28021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei285 – 30521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei338 – 35821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei376 – 39621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei451 – 47121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei510 – 53021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei576 – 59621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei659 – 67921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei811 – 83121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the SLC12A transporter family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0531.
    HOGENOMiHOG000062855.
    HOVERGENiHBG052851.
    InParanoidiP59158.
    KOiK14426.
    OMAiQLYPLIG.
    OrthoDBiEOG75MVVC.
    PhylomeDBiP59158.
    TreeFamiTF313191.

    Family and domain databases

    InterProiIPR004841. AA-permease/SLC12A_dom.
    IPR013612. AA_permease_N.
    IPR004842. Na/K/Cl_cotransptS.
    IPR002948. NaCl_cotranspt.
    [Graphical view]
    PfamiPF00324. AA_permease. 1 hit.
    PF08403. AA_permease_N. 1 hit.
    [Graphical view]
    PRINTSiPR01230. NACLTRNSPORT.
    TIGRFAMsiTIGR00930. 2a30. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P59158-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAELPVTELP GDALCSGRFT ISTLMGGDEP PPAACDSSQP SHLTHGSTLY     50
    MRTFGYNTID VVPAYEHYAN SALPGEPRKV RPTLADLHSF LKQEGSHLHA 100
    LAFDGRQGRE LTDGLVEDET GTNSEKSPGE PVRFGWVKGV MIRCMLNIWG 150
    VILYLRLPWI TAQAGIVLTW LIILLSVMVT SITGLSISAI STNGKVKSGG 200
    TYFLISRSLG PELGGSIGLI FAFANAVGVA MHTVGFAETV RDLLQEYGTP 250
    IVDPINDIRI IGVVTVTVLL AISLAGMEWE SKAQVLFFLV IMVSFANYLV 300
    GTLIPASEDK ASKGFYSYHG DIFVQNLVPD WRGIDGSFFG MFSIFFPSAT 350
    GILAGANISG DLKDPAVAIP KGTLMAIFWT TISYLAISAT IGSCVVRDAS 400
    GDVNDTMTPG PGPCEGLACG YGWNFTECSQ QRSCRYGLIN YYQTMSMVSA 450
    FAPLITAGIF GATLSSALAC LVSAAKVFQC LCEDQLYPLI GFFGKGYGKN 500
    REPVRGYLLA YAIAVAFIII AELNTIAPII SNFFLCSYAL INFSCFHASI 550
    TNSPGWRPSF RYYSKWAALF GAVISVVIMF LLTWWAALIA IGVVLFLLLY 600
    VIYKKPEVNW GSSVQAGSYN LALSYSVGLN EVEDHIKNYR PQCLVLTGPP 650
    NFRPALVDFV STFTQNLSLM ICGHVLIGPG KQRVPELRLI ASGHTKWLNK 700
    RKIKAFYSDV IAEDLRSGVQ ILMQASGLGR MKPNILVVGF KRNWQSAHPA 750
    TVEDYIGVLH DAFDFNYGVC VMRMREGLNV SEALQTHTTP EALIQEEQAS 800
    TIFQSEQGKK TIDIYWLFDD GGLTLLIPYL LHRKKRWGKC KIRVFVGGQI 850
    NRMDEERKAI ISLLSKFRLG FHEVHVLPDI NQKPQAEHTK RFEDMIAPFR 900
    LNDGFKDEAT VTEMRRDCPW KISDEEINKN RIKSLRQVRL SEILLDYSRD 950
    AALIILTLPI GRKGKCPSSL YMAWLETLSQ DLRPPVLLIR GNQENVLTFY 1000
    CQ 1002
    Length:1,002
    Mass (Da):110,694
    Last modified:December 13, 2002 - v1
    Checksum:i61CB02500C9093B9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC038612 mRNA. Translation: AAH38612.1.
    CCDSiCCDS57632.1.
    RefSeqiNP_001192240.1. NM_001205311.1.
    UniGeneiMm.25804.

    Genome annotation databases

    EnsembliENSMUST00000034218; ENSMUSP00000034218; ENSMUSG00000031766.
    GeneIDi20497.
    KEGGimmu:20497.
    UCSCiuc009mwc.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC038612 mRNA. Translation: AAH38612.1 .
    CCDSi CCDS57632.1.
    RefSeqi NP_001192240.1. NM_001205311.1.
    UniGenei Mm.25804.

    3D structure databases

    ProteinModelPortali P59158.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203278. 1 interaction.
    IntActi P59158. 1 interaction.
    MINTi MINT-4133700.

    PTM databases

    PhosphoSitei P59158.

    Proteomic databases

    PaxDbi P59158.
    PRIDEi P59158.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034218 ; ENSMUSP00000034218 ; ENSMUSG00000031766 .
    GeneIDi 20497.
    KEGGi mmu:20497.
    UCSCi uc009mwc.2. mouse.

    Organism-specific databases

    CTDi 6559.
    MGIi MGI:108114. Slc12a3.

    Phylogenomic databases

    eggNOGi COG0531.
    HOGENOMi HOG000062855.
    HOVERGENi HBG052851.
    InParanoidi P59158.
    KOi K14426.
    OMAi QLYPLIG.
    OrthoDBi EOG75MVVC.
    PhylomeDBi P59158.
    TreeFami TF313191.

    Miscellaneous databases

    ChiTaRSi SLC12A3. mouse.
    NextBioi 298655.
    PROi P59158.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P59158.
    Bgeei P59158.
    CleanExi MM_SLC12A3.
    Genevestigatori P59158.

    Family and domain databases

    InterProi IPR004841. AA-permease/SLC12A_dom.
    IPR013612. AA_permease_N.
    IPR004842. Na/K/Cl_cotransptS.
    IPR002948. NaCl_cotranspt.
    [Graphical view ]
    Pfami PF00324. AA_permease. 1 hit.
    PF08403. AA_permease_N. 1 hit.
    [Graphical view ]
    PRINTSi PR01230. NACLTRNSPORT.
    TIGRFAMsi TIGR00930. 2a30. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Kidney.
    2. "RasGRP1 stimulation enhances ubiquitination and endocytosis of the sodium-chloride cotransporter."
      Ko B., Kamsteeg E.J., Cooke L.L., Moddes L.N., Deen P.M., Hoover R.S.
      Am. J. Physiol. 299:F300-F309(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: UBIQUITINATION.

    Entry informationi

    Entry nameiS12A3_MOUSE
    AccessioniPrimary (citable) accession number: P59158
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 13, 2002
    Last sequence update: December 13, 2002
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3