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P59158

- S12A3_MOUSE

UniProt

P59158 - S12A3_MOUSE

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Protein

Solute carrier family 12 member 3

Gene

Slc12a3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Electrically silent transporter system. Mediates sodium and chloride reabsorption (By similarity).By similarity

Enzyme regulationi

Activated by WNK3.By similarity

GO - Molecular functioni

  1. cation:chloride symporter activity Source: InterPro
  2. transporter activity Source: UniProtKB

GO - Biological processi

  1. sodium ion transport Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

Sodium

Enzyme and pathway databases

ReactomeiREACT_247880. Cation-coupled Chloride cotransporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 12 member 3
Alternative name(s):
Na-Cl symporter
Thiazide-sensitive sodium-chloride cotransporter
Gene namesi
Name:Slc12a3
Synonyms:Ncc, Tsc
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:108114. Slc12a3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 133133CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei134 – 15421HelicalSequence AnalysisAdd
BLAST
Transmembranei165 – 18521HelicalSequence AnalysisAdd
BLAST
Topological domaini186 – 21631CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei217 – 23721HelicalSequence AnalysisAdd
BLAST
Transmembranei260 – 28021HelicalSequence AnalysisAdd
BLAST
Topological domaini281 – 2844CytoplasmicSequence Analysis
Transmembranei285 – 30521HelicalSequence AnalysisAdd
BLAST
Transmembranei338 – 35821HelicalSequence AnalysisAdd
BLAST
Topological domaini359 – 37517CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei376 – 39621HelicalSequence AnalysisAdd
BLAST
Transmembranei451 – 47121HelicalSequence AnalysisAdd
BLAST
Topological domaini472 – 50938CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei510 – 53021HelicalSequence AnalysisAdd
BLAST
Transmembranei576 – 59621HelicalSequence AnalysisAdd
BLAST
Topological domaini597 – 65862CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei659 – 67921HelicalSequence AnalysisAdd
BLAST
Transmembranei811 – 83121HelicalSequence AnalysisAdd
BLAST
Topological domaini832 – 1002171CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB
  2. cytosol Source: UniProtKB
  3. extracellular vesicular exosome Source: Ensembl
  4. integral component of membrane Source: UniProtKB-KW
  5. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10021002Solute carrier family 12 member 3PRO_0000178027Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi404 – 4041N-linked (GlcNAc...)Sequence Analysis
Glycosylationi424 – 4241N-linked (GlcNAc...)Sequence Analysis
Glycosylationi542 – 5421N-linked (GlcNAc...)Sequence Analysis
Glycosylationi779 – 7791N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Ubiquitinated; ubiquitination is essential for regulation of endocytosis.1 Publication

Keywords - PTMi

Glycoprotein, Ubl conjugation

Proteomic databases

PaxDbiP59158.
PRIDEiP59158.

PTM databases

PhosphoSiteiP59158.

Expressioni

Gene expression databases

BgeeiP59158.
CleanExiMM_SLC12A3.
ExpressionAtlasiP59158. baseline.
GenevestigatoriP59158.

Interactioni

Subunit structurei

Interacts with KLHL3.By similarity

Protein-protein interaction databases

BioGridi203278. 1 interaction.
IntActiP59158. 1 interaction.
MINTiMINT-4133700.

Structurei

3D structure databases

ProteinModelPortaliP59158.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the SLC12A transporter family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0531.
HOGENOMiHOG000062855.
HOVERGENiHBG052851.
InParanoidiP59158.
KOiK14426.
OMAiQLYPLIG.
OrthoDBiEOG75MVVC.
PhylomeDBiP59158.
TreeFamiTF313191.

Family and domain databases

InterProiIPR004841. AA-permease/SLC12A_dom.
IPR013612. AA_permease_N.
IPR004842. Na/K/Cl_cotransptS.
IPR002948. NaCl_cotranspt.
[Graphical view]
PfamiPF00324. AA_permease. 1 hit.
PF08403. AA_permease_N. 1 hit.
[Graphical view]
PRINTSiPR01230. NACLTRNSPORT.
TIGRFAMsiTIGR00930. 2a30. 1 hit.

Sequencei

Sequence statusi: Complete.

P59158-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAELPVTELP GDALCSGRFT ISTLMGGDEP PPAACDSSQP SHLTHGSTLY
60 70 80 90 100
MRTFGYNTID VVPAYEHYAN SALPGEPRKV RPTLADLHSF LKQEGSHLHA
110 120 130 140 150
LAFDGRQGRE LTDGLVEDET GTNSEKSPGE PVRFGWVKGV MIRCMLNIWG
160 170 180 190 200
VILYLRLPWI TAQAGIVLTW LIILLSVMVT SITGLSISAI STNGKVKSGG
210 220 230 240 250
TYFLISRSLG PELGGSIGLI FAFANAVGVA MHTVGFAETV RDLLQEYGTP
260 270 280 290 300
IVDPINDIRI IGVVTVTVLL AISLAGMEWE SKAQVLFFLV IMVSFANYLV
310 320 330 340 350
GTLIPASEDK ASKGFYSYHG DIFVQNLVPD WRGIDGSFFG MFSIFFPSAT
360 370 380 390 400
GILAGANISG DLKDPAVAIP KGTLMAIFWT TISYLAISAT IGSCVVRDAS
410 420 430 440 450
GDVNDTMTPG PGPCEGLACG YGWNFTECSQ QRSCRYGLIN YYQTMSMVSA
460 470 480 490 500
FAPLITAGIF GATLSSALAC LVSAAKVFQC LCEDQLYPLI GFFGKGYGKN
510 520 530 540 550
REPVRGYLLA YAIAVAFIII AELNTIAPII SNFFLCSYAL INFSCFHASI
560 570 580 590 600
TNSPGWRPSF RYYSKWAALF GAVISVVIMF LLTWWAALIA IGVVLFLLLY
610 620 630 640 650
VIYKKPEVNW GSSVQAGSYN LALSYSVGLN EVEDHIKNYR PQCLVLTGPP
660 670 680 690 700
NFRPALVDFV STFTQNLSLM ICGHVLIGPG KQRVPELRLI ASGHTKWLNK
710 720 730 740 750
RKIKAFYSDV IAEDLRSGVQ ILMQASGLGR MKPNILVVGF KRNWQSAHPA
760 770 780 790 800
TVEDYIGVLH DAFDFNYGVC VMRMREGLNV SEALQTHTTP EALIQEEQAS
810 820 830 840 850
TIFQSEQGKK TIDIYWLFDD GGLTLLIPYL LHRKKRWGKC KIRVFVGGQI
860 870 880 890 900
NRMDEERKAI ISLLSKFRLG FHEVHVLPDI NQKPQAEHTK RFEDMIAPFR
910 920 930 940 950
LNDGFKDEAT VTEMRRDCPW KISDEEINKN RIKSLRQVRL SEILLDYSRD
960 970 980 990 1000
AALIILTLPI GRKGKCPSSL YMAWLETLSQ DLRPPVLLIR GNQENVLTFY

CQ
Length:1,002
Mass (Da):110,694
Last modified:December 13, 2002 - v1
Checksum:i61CB02500C9093B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC038612 mRNA. Translation: AAH38612.1.
CCDSiCCDS57632.1.
RefSeqiNP_001192240.1. NM_001205311.1.
UniGeneiMm.25804.

Genome annotation databases

EnsembliENSMUST00000034218; ENSMUSP00000034218; ENSMUSG00000031766.
GeneIDi20497.
KEGGimmu:20497.
UCSCiuc009mwc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC038612 mRNA. Translation: AAH38612.1 .
CCDSi CCDS57632.1.
RefSeqi NP_001192240.1. NM_001205311.1.
UniGenei Mm.25804.

3D structure databases

ProteinModelPortali P59158.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 203278. 1 interaction.
IntActi P59158. 1 interaction.
MINTi MINT-4133700.

PTM databases

PhosphoSitei P59158.

Proteomic databases

PaxDbi P59158.
PRIDEi P59158.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000034218 ; ENSMUSP00000034218 ; ENSMUSG00000031766 .
GeneIDi 20497.
KEGGi mmu:20497.
UCSCi uc009mwc.2. mouse.

Organism-specific databases

CTDi 6559.
MGIi MGI:108114. Slc12a3.

Phylogenomic databases

eggNOGi COG0531.
HOGENOMi HOG000062855.
HOVERGENi HBG052851.
InParanoidi P59158.
KOi K14426.
OMAi QLYPLIG.
OrthoDBi EOG75MVVC.
PhylomeDBi P59158.
TreeFami TF313191.

Enzyme and pathway databases

Reactomei REACT_247880. Cation-coupled Chloride cotransporters.

Miscellaneous databases

ChiTaRSi Slc12a3. mouse.
NextBioi 298655.
PROi P59158.
SOURCEi Search...

Gene expression databases

Bgeei P59158.
CleanExi MM_SLC12A3.
ExpressionAtlasi P59158. baseline.
Genevestigatori P59158.

Family and domain databases

InterProi IPR004841. AA-permease/SLC12A_dom.
IPR013612. AA_permease_N.
IPR004842. Na/K/Cl_cotransptS.
IPR002948. NaCl_cotranspt.
[Graphical view ]
Pfami PF00324. AA_permease. 1 hit.
PF08403. AA_permease_N. 1 hit.
[Graphical view ]
PRINTSi PR01230. NACLTRNSPORT.
TIGRFAMsi TIGR00930. 2a30. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.
  2. "RasGRP1 stimulation enhances ubiquitination and endocytosis of the sodium-chloride cotransporter."
    Ko B., Kamsteeg E.J., Cooke L.L., Moddes L.N., Deen P.M., Hoover R.S.
    Am. J. Physiol. 299:F300-F309(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION.

Entry informationi

Entry nameiS12A3_MOUSE
AccessioniPrimary (citable) accession number: P59158
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 13, 2002
Last sequence update: December 13, 2002
Last modified: November 26, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3