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Protein

Copine-2

Gene

Cpne2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium-dependent phospholipid-binding protein that plays a role in calcium-mediated intracellular processes. Exhibits calcium-dependent cell membrane binding properties (PubMed:21087455, PubMed:26175110).2 Publications

GO - Biological processi

  • cellular response to calcium ion Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Copine-2Curated
Alternative name(s):
Copine IIBy similarityImported
Gene namesi
Name:Cpne2Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:2387578. Cpne2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: MGI
  • nucleus Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi37D → N: Does not inhibit calcium-dependent translocation to the cell membrane; when associated with N-39; N-97 and N-99. 1 Publication1
Mutagenesisi39D → N: Does not inhibit calcium-dependent translocation to the cell membrane; when associated with N-37; N-97 and N-99. 1 Publication1
Mutagenesisi97D → N: Does not inhibit calcium-dependent translocation to the cell membrane; when associated with N-37; N-39 and N-99. 1 Publication1
Mutagenesisi99D → N: Does not inhibit calcium-dependent translocation to the cell membrane; when associated with N-37; N-39 and N-97. 1 Publication1
Mutagenesisi170D → N: Leads to a reduction in the amount of calcium-dependent translocation to the cell membrane. Leads to the constitutive (calcium-independent) attachment to the cell membrane; when associated with N-176; N-232 and N-234. 1 Publication1
Mutagenesisi176D → N: Does not inhibit calcium-dependent translocation to the cell membrane. Leads to the constitutive (calcium-independent) attachment to the cell membrane; when associated with N-170; N-232 and N-234. 1 Publication1
Mutagenesisi232D → N: Leads to a reduction in the amount of calcium-dependent translocation to the cell membrane. Leads to the constitutive (calcium-independent) attachment to the cell membrane; when associated with N-170; N-176 and N-234. 1 Publication1
Mutagenesisi234D → N: Leads to a reduction in the amount of calcium-dependent translocation to the cell membrane. Leads to the constitutive (calcium-independent) attachment to the cell membrane; when associated with N-170; N-176 and N-232. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001448371 – 548Copine-2Add BLAST548

Proteomic databases

EPDiP59108.
MaxQBiP59108.
PaxDbiP59108.
PeptideAtlasiP59108.
PRIDEiP59108.

PTM databases

iPTMnetiP59108.
PhosphoSitePlusiP59108.

Expressioni

Gene expression databases

BgeeiENSMUSG00000034361.
CleanExiMM_CPNE2.
ExpressionAtlasiP59108. baseline and differential.
GenevisibleiP59108. MM.

Interactioni

Protein-protein interaction databases

BioGridi231541. 1 interactor.
IntActiP59108. 2 interactors.
MINTiMINT-8049162.
STRINGi10090.ENSMUSP00000045755.

Structurei

3D structure databases

ProteinModelPortaliP59108.
SMRiP59108.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 115C2 1PROSITE-ProRule annotationAdd BLAST106
Domaini141 – 246C2 2PROSITE-ProRule annotationAdd BLAST106
Domaini305 – 507VWFAPROSITE-ProRule annotationAdd BLAST203

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni247 – 304Linker region1 PublicationAdd BLAST58

Domaini

The C2 domain 2, but not the C2 domain 1, is necessary for calcium-mediated membrane association (PubMed:21087455, PubMed:26175110). The linker region is necessary for calcium-dependent cell membrane association (PubMed:21087455).2 Publications

Sequence similaritiesi

Belongs to the copine family.Curated
Contains 2 C2 domains.PROSITE-ProRule annotation
Contains 1 VWFA domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1327. Eukaryota.
ENOG410XPC8. LUCA.
GeneTreeiENSGT00760000119085.
HOGENOMiHOG000220898.
HOVERGENiHBG066841.
InParanoidiP59108.
OMAiMGPQYCV.
OrthoDBiEOG091G05IT.
PhylomeDBiP59108.
TreeFamiTF316419.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR010734. Copine.
IPR002035. VWF_A.
[Graphical view]
PfamiPF00168. C2. 2 hits.
PF07002. Copine. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 2 hits.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
SSF53300. SSF53300. 1 hit.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59108-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYIPDGGAP TAGAIPLGSQ CCVCKVELSV SGQNLLDRDV TSKSDPFCVL
60 70 80 90 100
FIEDNGRWME FDRTETAVNN LNPAFSKKFV LDYHFEEVQK LKFALFDQDK
110 120 130 140 150
SSAQLDEHDF LGQFSCSLGT IVSSKKITRP LLLMNDKPAG KGVITIAAQE
160 170 180 190 200
LSDNRVITLS LAGRKLDKKD LFGKSDPFLE FYKPGDDGKW MLVHRTEVIK
210 220 230 240 250
YTLDPVWKPF TVPLVSLCDG DLEKPIQVMC YDYDSNGGHD FIGEFQTSVL
260 270 280 290 300
QMSEARDGVP LEIECINPKK QRKKKSYKNS GIIILRSCKI HRNYSFLDYI
310 320 330 340 350
LGGCQLMFTV GIDFTASNGN PLDPSSLHYI NPMGTNEYLS AIWAVGQIIQ
360 370 380 390 400
DYDSDKMFPA LGFGAQLPPD WKVSHEFAIN FNPTNPFCSG VDGIAQAYSA
410 420 430 440 450
CLPHIRFYGP TNFSPIVNHV ARFAAQATQQ QTATQYFILL IITDGVISDM
460 470 480 490 500
EETRHAVVQA SKLPMSIIIV GVGNADFAAM EFLDGDNRRL RSHTGEEAAR
510 520 530 540
DIVQFVPFRE FRNAAKETLA KAVLAELPQQ VVQYFKHKNL PPTNSEPA
Length:548
Mass (Da):61,036
Last modified:November 28, 2002 - v1
Checksum:i796F26124D2A0E13
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti435Missing in AAH22128 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC022128 mRNA. Translation: AAH22128.2.
BC031801 mRNA. Translation: AAH31801.1.
CCDSiCCDS22543.1.
RefSeqiNP_705727.1. NM_153507.2.
UniGeneiMm.291815.

Genome annotation databases

EnsembliENSMUST00000048653; ENSMUSP00000045755; ENSMUSG00000034361.
GeneIDi234577.
KEGGimmu:234577.
UCSCiuc009mwm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC022128 mRNA. Translation: AAH22128.2.
BC031801 mRNA. Translation: AAH31801.1.
CCDSiCCDS22543.1.
RefSeqiNP_705727.1. NM_153507.2.
UniGeneiMm.291815.

3D structure databases

ProteinModelPortaliP59108.
SMRiP59108.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231541. 1 interactor.
IntActiP59108. 2 interactors.
MINTiMINT-8049162.
STRINGi10090.ENSMUSP00000045755.

PTM databases

iPTMnetiP59108.
PhosphoSitePlusiP59108.

Proteomic databases

EPDiP59108.
MaxQBiP59108.
PaxDbiP59108.
PeptideAtlasiP59108.
PRIDEiP59108.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048653; ENSMUSP00000045755; ENSMUSG00000034361.
GeneIDi234577.
KEGGimmu:234577.
UCSCiuc009mwm.1. mouse.

Organism-specific databases

CTDi221184.
MGIiMGI:2387578. Cpne2.

Phylogenomic databases

eggNOGiKOG1327. Eukaryota.
ENOG410XPC8. LUCA.
GeneTreeiENSGT00760000119085.
HOGENOMiHOG000220898.
HOVERGENiHBG066841.
InParanoidiP59108.
OMAiMGPQYCV.
OrthoDBiEOG091G05IT.
PhylomeDBiP59108.
TreeFamiTF316419.

Miscellaneous databases

PROiP59108.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034361.
CleanExiMM_CPNE2.
ExpressionAtlasiP59108. baseline and differential.
GenevisibleiP59108. MM.

Family and domain databases

Gene3Di2.60.40.150. 2 hits.
InterProiIPR000008. C2_dom.
IPR010734. Copine.
IPR002035. VWF_A.
[Graphical view]
PfamiPF00168. C2. 2 hits.
PF07002. Copine. 1 hit.
[Graphical view]
SMARTiSM00239. C2. 2 hits.
SM00327. VWA. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 2 hits.
SSF53300. SSF53300. 1 hit.
PROSITEiPS50004. C2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCPNE2_MOUSE
AccessioniPrimary (citable) accession number: P59108
Secondary accession number(s): Q8K1D7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: November 28, 2002
Last modified: November 30, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.