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Protein

Photosystem II reaction center protein J

Gene

psbJ

Organism
Thermosynechococcus elongatus (strain BP-1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

One of the components of the core complex of photosystem II (PSII). PSII is a light-driven water:plastoquinone oxidoreductase that uses light energy to abstract electrons from H2O, generating O2 and a proton gradient subsequently used for ATP formation. It consists of a core antenna complex that captures photons, and an electron transfer chain that converts photonic excitation into a charge separation.UniRule annotation4 Publications

Cofactori

Note: PSII binds multiple chlorophylls, carotenoids and specific lipids.9 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Photosynthesis

Names & Taxonomyi

Protein namesi
Recommended name:
Photosystem II reaction center protein JUniRule annotation
Short name:
PSII-JUniRule annotation
Gene namesi
Name:psbJUniRule annotation
Ordered Locus Names:tsr1544
OrganismiThermosynechococcus elongatus (strain BP-1)
Taxonomic identifieri197221 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesThermosynechococcus
Proteomesi
  • UP000000440 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 1110Cytoplasmic1 Publication
Transmembranei12 – 2615Helical1 PublicationAdd
BLAST
Topological domaini27 – 4014Lumenal1 PublicationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Photosystem II, Reaction center, Thylakoid

Pathology & Biotechi

Disruption phenotypei

No change in growth rate or oxygen evolution under standard growth conditions (50 µmol photons/m2/s and 45 degrees Celsius). Dimeric PSII less stable upon isolation, soluble protein Psb28 associates substoichiometrically with PSII. Only the D1 protein translated from the psbA2 or psbA3 gene is found in PSII.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved2 Publications
Chaini2 – 4039Photosystem II reaction center protein JPRO_0000216623Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylmethionine2 Publications

Keywords - PTMi

Acetylation

Interactioni

Subunit structurei

Cyanobacterial PSII is composed of 1 copy each of membrane proteins PsbA, PsbB, PsbC, PsbD, PsbE, PsbF, PsbH, PsbI, PsbJ, PsbK, PsbL, PsbM, PsbT, PsbX, PsbY, PsbZ, Ycf12, at least 3 peripheral proteins PsbO, PsbU, PsbV and a large number of cofactors. It forms dimeric complexes.UniRule annotation10 Publications

Protein-protein interaction databases

DIPiDIP-48495N.
STRINGi197221.tsr1544.

Structurei

Secondary structure

1
40
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi10 – 3122Combined sources
Beta strandi33 – 353Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1S5LX-ray3.50J/j1-40[»]
2AXTX-ray3.00J/j1-40[»]
3KZIX-ray3.60J1-40[»]
4FBYX-ray6.56J/b2-40[»]
4IXQX-ray5.70J/j1-40[»]
4IXRX-ray5.90J/j1-40[»]
4PBUX-ray5.00J/j1-40[»]
4PJ0X-ray2.44J/j1-40[»]
4RVYX-ray5.50J/j1-40[»]
4TNHX-ray4.90J/j1-40[»]
4TNIX-ray4.60J/j1-40[»]
4TNJX-ray4.50J/j1-40[»]
4TNKX-ray5.20J/j1-40[»]
4V62X-ray2.90AJ/BJ1-40[»]
4V82X-ray3.20AJ/BJ1-40[»]
5E7CX-ray4.50J/j3-40[»]
ProteinModelPortaliP59087.
SMRiP59087. Positions 3-40.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP59087.

Family & Domainsi

Sequence similaritiesi

Belongs to the PsbJ family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4106DN3. Bacteria.
ENOG410XW1C. LUCA.
HOGENOMiHOG000035621.
KOiK02711.
OrthoDBiEOG6MSS9B.

Family and domain databases

HAMAPiMF_01305. PSII_PsbJ.
InterProiIPR002682. PSII_PsbJ.
[Graphical view]
PfamiPF01788. PsbJ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P59087-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40
MMSEGGRIPL WIVATVAGMG VIVIVGLFFY GAYAGLGSSL
Length:40
Mass (Da):4,105
Last modified:November 8, 2002 - v1
Checksum:i4D05FAA8C690069E
GO

Mass spectrometryi

Molecular mass is 4017±2 Da from positions 2 - 39. Determined by MALDI. 1 Publication
Molecular mass is 4017 Da from positions 1 - 40. Determined by MALDI. 1 Publication
Molecular mass is 4002 Da from positions 2 - 40. Determined by MALDI. Suggests the protein is 1 residue shorter at the N-terminus and N-formylated.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000039 Genomic DNA. Translation: BAC09096.1.
RefSeqiNP_682334.1. NC_004113.1.
WP_011057384.1. NC_004113.1.

Genome annotation databases

EnsemblBacteriaiBAC09096; BAC09096; BAC09096.
GeneIDi1011064.
KEGGitel:tsr1544.
PATRICi23928452. VBITheElo119873_1620.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000039 Genomic DNA. Translation: BAC09096.1.
RefSeqiNP_682334.1. NC_004113.1.
WP_011057384.1. NC_004113.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1S5LX-ray3.50J/j1-40[»]
2AXTX-ray3.00J/j1-40[»]
3KZIX-ray3.60J1-40[»]
4FBYX-ray6.56J/b2-40[»]
4IXQX-ray5.70J/j1-40[»]
4IXRX-ray5.90J/j1-40[»]
4PBUX-ray5.00J/j1-40[»]
4PJ0X-ray2.44J/j1-40[»]
4RVYX-ray5.50J/j1-40[»]
4TNHX-ray4.90J/j1-40[»]
4TNIX-ray4.60J/j1-40[»]
4TNJX-ray4.50J/j1-40[»]
4TNKX-ray5.20J/j1-40[»]
4V62X-ray2.90AJ/BJ1-40[»]
4V82X-ray3.20AJ/BJ1-40[»]
5E7CX-ray4.50J/j3-40[»]
ProteinModelPortaliP59087.
SMRiP59087. Positions 3-40.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48495N.
STRINGi197221.tsr1544.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC09096; BAC09096; BAC09096.
GeneIDi1011064.
KEGGitel:tsr1544.
PATRICi23928452. VBITheElo119873_1620.

Phylogenomic databases

eggNOGiENOG4106DN3. Bacteria.
ENOG410XW1C. LUCA.
HOGENOMiHOG000035621.
KOiK02711.
OrthoDBiEOG6MSS9B.

Miscellaneous databases

EvolutionaryTraceiP59087.

Family and domain databases

HAMAPiMF_01305. PSII_PsbJ.
InterProiIPR002682. PSII_PsbJ.
[Graphical view]
PfamiPF01788. PsbJ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BP-1.
  2. "Deletion of psbJ leads to accumulation of Psb27-Psb28 photosystem II complexes in Thermosynechococcus elongatus."
    Nowaczyk M.M., Krause K., Mieseler M., Sczibilanski A., Ikeuchi M., Roegner M.
    Biochim. Biophys. Acta 1817:1339-1345(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, MASS SPECTROMETRY, DISRUPTION PHENOTYPE.
    Strain: BP-1.
  3. "Architecture of the photosynthetic oxygen-evolving center."
    Ferreira K.N., Iverson T.M., Maghlaoui K., Barber J., Iwata S.
    Science 303:1831-1838(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.50 ANGSTROMS) IN PHOTOSYSTEM II, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION.
  4. "Towards complete cofactor arrangement in the 3.0 A resolution structure of photosystem II."
    Loll B., Kern J., Saenger W., Zouni A., Biesiadka J.
    Nature 438:1040-1044(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.00 ANGSTROMS) IN PHOTOSYSTEM II, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION.
    Strain: BP-1.
  5. "Cyanobacterial photosystem II at 2.9-A resolution and the role of quinones, lipids, channels and chloride."
    Guskov A., Kern J., Gabdulkhakov A., Broser M., Zouni A., Saenger W.
    Nat. Struct. Mol. Biol. 16:334-342(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS) IN PHOTOSYSTEM II, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION, MASS SPECTROMETRY, TOPOLOGY.
    Strain: BP-1.
  6. "Crystal structure of monomeric photosystem II from Thermosynechococcus elongatus at 3.6 A resolution."
    Broser M., Gabdulkhakov A., Kern J., Guskov A., Muh F., Saenger W., Zouni A.
    J. Biol. Chem. 285:26255-26262(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.60 ANGSTROMS) IN PHOTOSYSTEM II, FUNCTION, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION, MASS SPECTROMETRY.
    Strain: BP-1.
  7. "Structural basis of cyanobacterial photosystem II inhibition by the herbicide terbutryn."
    Broser M., Glockner C., Gabdulkhakov A., Guskov A., Buchta J., Kern J., Muh F., Dau H., Saenger W., Zouni A.
    J. Biol. Chem. 286:15964-15972(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.20 ANGSTROMS) IN PHOTOSYSTEM II, FUNCTION, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION.
  8. Cited for: X-RAY CRYSTALLOGRAPHY (6.56 ANGSTROMS) OF 2-40 IN PHOTOSYSTEM II, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION.
    Strain: BP-1.
  9. Cited for: X-RAY CRYSTALLOGRAPHY (5.70 ANGSTROMS) IN PHOTOSYSTEM II, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION.
    Strain: BP-1.
  10. "Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser."
    Kupitz C., Basu S., Grotjohann I., Fromme R., Zatsepin N.A., Rendek K.N., Hunter M.S., Shoeman R.L., White T.A., Wang D., James D., Yang J.H., Cobb D.E., Reeder B., Sierra R.G., Liu H., Barty A., Aquila A.L.
    , Deponte D., Kirian R.A., Bari S., Bergkamp J.J., Beyerlein K.R., Bogan M.J., Caleman C., Chao T.C., Conrad C.E., Davis K.M., Fleckenstein H., Galli L., Hau-Riege S.P., Kassemeyer S., Laksmono H., Liang M., Lomb L., Marchesini S., Martin A.V., Messerschmidt M., Milathianaki D., Nass K., Ros A., Roy-Chowdhury S., Schmidt K., Seibert M., Steinbrener J., Stellato F., Yan L., Yoon C., Moore T.A., Moore A.L., Pushkar Y., Williams G.J., Boutet S., Doak R.B., Weierstall U., Frank M., Chapman H.N., Spence J.C., Fromme P.
    Nature 513:261-265(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (5.00 ANGSTROMS) IN PHOTOSYSTEM II, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION.
    Strain: BP-1.
  11. Cited for: X-RAY CRYSTALLOGRAPHY (4.50 ANGSTROMS) IN PHOTOSYSTEM II, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION.
    Strain: BP-1.

Entry informationi

Entry nameiPSBJ_THEEB
AccessioniPrimary (citable) accession number: P59087
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: November 8, 2002
Last modified: April 13, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.