P59082 (LFS_ALLCE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lachrymatory-factor synthase

Gene names

Name:

LFS

Organism

Allium cepa (Onion)

Taxonomic identifier

4679 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › Asparagales › Amaryllidaceae › Allioideae › Allieae › Allium

Protein attributes

Sequence length	169 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Produces lacrymatory factor (propanthial S-oxide) from 1-propenylsulphenic acid, an unstable compound resulting from the degradation of trans-1-propenyl-L-cysteine sulphoxide (PRENCSO) by alliinase.
Subcellular location	Vacuole Probable.
Biotechnological use	It may be possible to develop a non-lachrymal onion that still retains its characteristic flavor and high nutritional value by down-regulating the activity of this enzyme.

Ontologies

Keywords
Cellular component	Vacuole
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 12	12			PRO_0000021591
	Chain	13 – 169	157	Lachrymatory-factor synthase		PRO_0000021592

Sequences

	Sequence		Length	Mass (Da)
	P59082 [UniParc]. Last modified November 8, 2002. Version 1. Checksum: D1D2DBD00A244EF1	FASTA	169	19,057
	10 20 30 40 50 60 MELNPGAPAV VADSANGARK WSGKVHALLP NTKPEQAWTL LKDFINLHKV MPSLSVCELV 70 80 90 100 110 120 EGEANVVGCV RYVKGIMHPI EEEFWAKEKL VALDNKNMSY SYIFTECFTG YEDYTATMQI 130 140 150 160 VEGPEHKGSR FDWSFQCKYI EGMTESAFTE ILQHWATEIG QKIEEVCSA

References

[1]	"An onion enzyme that makes the eyes water." Imai S., Tsuge N., Tomotake M., Nagatome Y., Sawada H., Nagata T., Kumagai H. Nature 419:685-685(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 13-22.

Web resources

Protein Spotlight

No LFS, no cry - Issue 28 of November 2002

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB089203 mRNA. Translation: BAC21275.1.
3D structure databases
ProteinModelPortal	P59082.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13498.
Family and domain databases
Gene3D	3.30.530.20. 1 hit.
InterPro	IPR019587. Polyketide_cyclase/dehydratase. IPR023393. START-like_dom. [Graphical view]
Pfam	PF10604. Polyketide_cyc2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LFS_ALLCE

Accession

Primary (citable) accession number: P59082

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	November 8, 2002
Last modified:	April 3, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program