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P59078 (SYM_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase

EC=6.1.1.10
Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS
Gene names
Name:metG
Ordered Locus Names:BR0995, BS1330_I0991
OrganismBrucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]
Taxonomic identifier204722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length515 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_01228

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_01228

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01228

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01228.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processmethionyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

methionine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 515515Methionine--tRNA ligase HAMAP-Rule MF_01228
PRO_0000139213

Regions

Motif13 – 2311"HIGH" region HAMAP-Rule MF_01228
Motif300 – 3045"KMSKS" region HAMAP-Rule MF_01228

Sites

Binding site3031ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P59078 [UniParc].

Last modified November 8, 2002. Version 1.
Checksum: B3CBD8FA93515199

FASTA51557,998
        10         20         30         40         50         60 
MSREKYYITT AIAYPNGKPH IGHAYELIAT DAMARFQRLN GMDVYFLTGT DEHGIKMLQS 

        70         80         90        100        110        120 
ARKEGITPRE LADRNTSAFR RMAEVLNSSN DDYIRTSEER HYKASQAIWQ AMVANGDIYK 

       130        140        150        160        170        180 
GGYAGWYSVR DEAYYGEEET EVRADGVRYG PQGTPVEWVE EESYFFRLSA YQDKLLDLYE 

       190        200        210        220        230        240 
NNPGFIMPAE RRNEIVSFVK SGLKDLSISR TTFDWGIPVP GDEKHVMYVW VDALTNYITA 

       250        260        270        280        290        300 
LGYPDTTDER WAYWPANAHI IGKDISRFHA VYWPAFLMSA QLPLPKRVFA HGFLFNRGEK 

       310        320        330        340        350        360 
MSKSVGNVID PFELVERYGL DQLRYFLMRE VPFGQDGSYS HEAIVNRTNA DLANDLGNLA 

       370        380        390        400        410        420 
QRSLSMIAKN CEGKVPQPGA FSEADKAILD QADAALETAR KAMDDQALHL ALGAIFAVVA 

       430        440        450        460        470        480 
EANRYFAGQE PWALRKTDPA RMGTVLYVTA EVLRRVGIMV QPFIPQSAEK LLDILAVPAD 

       490        500        510 
KRQFADVLAS PLAGGTDLPA PQPVFPRYVE ADEQN 

« Hide

References

[1]"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts."
Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. expand/collapse author list , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.
[2]"Revised genome sequence of Brucella suis 1330."
Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.
J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014291 Genomic DNA. Translation: AAN29918.1.
CP002997 Genomic DNA. Translation: AEM18335.1.
RefSeqNP_698003.1. NC_004310.3.
YP_005615827.1. NC_017251.1.

3D structure databases

ProteinModelPortalP59078.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING204722.BR0995.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN29918; AAN29918; BR0995.
AEM18335; AEM18335; BS1330_I0991.
GeneID1166668.
12138021.
KEGGbms:BR0995.
bsi:BS1330_I0991.
PATRIC17790302. VBIBruSui107850_1007.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0143.
HOGENOMHOG000200401.
KOK01874.
OMAEHGQKMQ.
OrthoDBEOG6CVV9B.
ProtClustDBPRK11893.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_01228. Met_tRNA_synth_type2.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF47323. SSF47323. 1 hit.
TIGRFAMsTIGR00398. metG. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYM_BRUSU
AccessionPrimary (citable) accession number: P59078
Secondary accession number(s): G0K9R9
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: November 8, 2002
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Brucella suis

Brucella suis (strain 1330): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries