Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Methionine--tRNA ligase

Gene

metG

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.UniRule annotation

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei303 – 3031ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligaseUniRule annotation (EC:6.1.1.10UniRule annotation)
Alternative name(s):
Methionyl-tRNA synthetaseUniRule annotation
Short name:
MetRSUniRule annotation
Gene namesi
Name:metGUniRule annotation
Ordered Locus Names:BR0995, BS1330_I0991
OrganismiBrucella suis biovar 1 (strain 1330)
Taxonomic identifieri204722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000007104 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 515515Methionine--tRNA ligasePRO_0000139213Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

Secondary structure

1
515
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 106Combined sources
Helixi21 – 3919Combined sources
Beta strandi43 – 508Combined sources
Helixi55 – 6410Combined sources
Helixi68 – 8518Combined sources
Beta strandi91 – 955Combined sources
Helixi99 – 11416Combined sources
Beta strandi118 – 1225Combined sources
Beta strandi125 – 1284Combined sources
Turni129 – 1324Combined sources
Beta strandi133 – 1353Combined sources
Helixi137 – 1393Combined sources
Beta strandi153 – 1553Combined sources
Beta strandi157 – 1593Combined sources
Beta strandi162 – 1665Combined sources
Helixi168 – 1714Combined sources
Helixi172 – 18110Combined sources
Beta strandi185 – 1884Combined sources
Helixi189 – 20012Combined sources
Beta strandi209 – 2124Combined sources
Beta strandi214 – 2163Combined sources
Beta strandi222 – 2276Combined sources
Helixi229 – 2346Combined sources
Helixi236 – 2394Combined sources
Turni240 – 2445Combined sources
Helixi249 – 2535Combined sources
Beta strandi257 – 2626Combined sources
Helixi263 – 2653Combined sources
Helixi266 – 2705Combined sources
Helixi272 – 2798Combined sources
Beta strandi288 – 2914Combined sources
Beta strandi294 – 2963Combined sources
Helixi311 – 3188Combined sources
Helixi320 – 33011Combined sources
Beta strandi337 – 3393Combined sources
Helixi341 – 35111Combined sources
Turni352 – 3554Combined sources
Helixi356 – 37015Combined sources
Helixi383 – 40422Combined sources
Helixi408 – 42922Combined sources
Helixi431 – 4377Combined sources
Helixi439 – 46022Combined sources
Turni461 – 4633Combined sources
Helixi465 – 47410Combined sources
Helixi479 – 4813Combined sources
Helixi486 – 4894Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5K0SX-ray2.45A/B/C1-515[»]
5K0TX-ray2.60A/B/C1-515[»]
ProteinModelPortaliP59078.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi13 – 2311"HIGH" regionAdd
BLAST
Motifi300 – 3045"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000200401.
KOiK01874.
OMAiHTFYVWF.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_01228. Met_tRNA_synth_type2. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59078-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSREKYYITT AIAYPNGKPH IGHAYELIAT DAMARFQRLN GMDVYFLTGT
60 70 80 90 100
DEHGIKMLQS ARKEGITPRE LADRNTSAFR RMAEVLNSSN DDYIRTSEER
110 120 130 140 150
HYKASQAIWQ AMVANGDIYK GGYAGWYSVR DEAYYGEEET EVRADGVRYG
160 170 180 190 200
PQGTPVEWVE EESYFFRLSA YQDKLLDLYE NNPGFIMPAE RRNEIVSFVK
210 220 230 240 250
SGLKDLSISR TTFDWGIPVP GDEKHVMYVW VDALTNYITA LGYPDTTDER
260 270 280 290 300
WAYWPANAHI IGKDISRFHA VYWPAFLMSA QLPLPKRVFA HGFLFNRGEK
310 320 330 340 350
MSKSVGNVID PFELVERYGL DQLRYFLMRE VPFGQDGSYS HEAIVNRTNA
360 370 380 390 400
DLANDLGNLA QRSLSMIAKN CEGKVPQPGA FSEADKAILD QADAALETAR
410 420 430 440 450
KAMDDQALHL ALGAIFAVVA EANRYFAGQE PWALRKTDPA RMGTVLYVTA
460 470 480 490 500
EVLRRVGIMV QPFIPQSAEK LLDILAVPAD KRQFADVLAS PLAGGTDLPA
510
PQPVFPRYVE ADEQN
Length:515
Mass (Da):57,998
Last modified:November 8, 2002 - v1
Checksum:iB3CBD8FA93515199
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN29918.1.
CP002997 Genomic DNA. Translation: AEM18335.1.
RefSeqiWP_004689678.1. NZ_KN046804.1.

Genome annotation databases

EnsemblBacteriaiAAN29918; AAN29918; BR0995.
AEM18335; AEM18335; BS1330_I0991.
KEGGibms:BR0995.
bsi:BS1330_I0991.
PATRICi17790302. VBIBruSui107850_1007.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN29918.1.
CP002997 Genomic DNA. Translation: AEM18335.1.
RefSeqiWP_004689678.1. NZ_KN046804.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5K0SX-ray2.45A/B/C1-515[»]
5K0TX-ray2.60A/B/C1-515[»]
ProteinModelPortaliP59078.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN29918; AAN29918; BR0995.
AEM18335; AEM18335; BS1330_I0991.
KEGGibms:BR0995.
bsi:BS1330_I0991.
PATRICi17790302. VBIBruSui107850_1007.

Phylogenomic databases

HOGENOMiHOG000200401.
KOiK01874.
OMAiHTFYVWF.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_01228. Met_tRNA_synth_type2. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYM_BRUSU
AccessioniPrimary (citable) accession number: P59078
Secondary accession number(s): G0K9R9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: November 8, 2002
Last modified: September 7, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Brucella suis
    Brucella suis (strain 1330): entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.