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Protein

Methionine--tRNA ligase

Gene

metG

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.UniRule annotation

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei303 – 3031ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. methionine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. methionyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligaseUniRule annotation (EC:6.1.1.10UniRule annotation)
Alternative name(s):
Methionyl-tRNA synthetaseUniRule annotation
Short name:
MetRSUniRule annotation
Gene namesi
Name:metGUniRule annotation
Ordered Locus Names:BR0995, BS1330_I0991
OrganismiBrucella suis biovar 1 (strain 1330)
Taxonomic identifieri204722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000007104 Componenti: Chromosome I UP000000824 Componenti: Chromosome I

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 515515Methionine--tRNA ligasePRO_0000139213Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi204722.BR0995.

Structurei

3D structure databases

ProteinModelPortaliP59078.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi13 – 2311"HIGH" regionAdd
BLAST
Motifi300 – 3045"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0143.
HOGENOMiHOG000200401.
KOiK01874.
OMAiGVFPRYV.
OrthoDBiEOG6CVV9B.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_01228. Met_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59078-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSREKYYITT AIAYPNGKPH IGHAYELIAT DAMARFQRLN GMDVYFLTGT
60 70 80 90 100
DEHGIKMLQS ARKEGITPRE LADRNTSAFR RMAEVLNSSN DDYIRTSEER
110 120 130 140 150
HYKASQAIWQ AMVANGDIYK GGYAGWYSVR DEAYYGEEET EVRADGVRYG
160 170 180 190 200
PQGTPVEWVE EESYFFRLSA YQDKLLDLYE NNPGFIMPAE RRNEIVSFVK
210 220 230 240 250
SGLKDLSISR TTFDWGIPVP GDEKHVMYVW VDALTNYITA LGYPDTTDER
260 270 280 290 300
WAYWPANAHI IGKDISRFHA VYWPAFLMSA QLPLPKRVFA HGFLFNRGEK
310 320 330 340 350
MSKSVGNVID PFELVERYGL DQLRYFLMRE VPFGQDGSYS HEAIVNRTNA
360 370 380 390 400
DLANDLGNLA QRSLSMIAKN CEGKVPQPGA FSEADKAILD QADAALETAR
410 420 430 440 450
KAMDDQALHL ALGAIFAVVA EANRYFAGQE PWALRKTDPA RMGTVLYVTA
460 470 480 490 500
EVLRRVGIMV QPFIPQSAEK LLDILAVPAD KRQFADVLAS PLAGGTDLPA
510
PQPVFPRYVE ADEQN
Length:515
Mass (Da):57,998
Last modified:November 8, 2002 - v1
Checksum:iB3CBD8FA93515199
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN29918.1.
CP002997 Genomic DNA. Translation: AEM18335.1.
RefSeqiNP_698003.1. NC_004310.3.
YP_005615827.1. NC_017251.1.

Genome annotation databases

EnsemblBacteriaiAAN29918; AAN29918; BR0995.
AEM18335; AEM18335; BS1330_I0991.
KEGGibms:BR0995.
bsi:BS1330_I0991.
PATRICi17790302. VBIBruSui107850_1007.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN29918.1.
CP002997 Genomic DNA. Translation: AEM18335.1.
RefSeqiNP_698003.1. NC_004310.3.
YP_005615827.1. NC_017251.1.

3D structure databases

ProteinModelPortaliP59078.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi204722.BR0995.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN29918; AAN29918; BR0995.
AEM18335; AEM18335; BS1330_I0991.
KEGGibms:BR0995.
bsi:BS1330_I0991.
PATRICi17790302. VBIBruSui107850_1007.

Phylogenomic databases

eggNOGiCOG0143.
HOGENOMiHOG000200401.
KOiK01874.
OMAiGVFPRYV.
OrthoDBiEOG6CVV9B.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_01228. Met_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1330.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1330.

Entry informationi

Entry nameiSYM_BRUSU
AccessioniPrimary (citable) accession number: P59078
Secondary accession number(s): G0K9R9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: November 8, 2002
Last modified: April 1, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Brucella suis
    Brucella suis (strain 1330): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.