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Protein

Methionine--tRNA ligase

Gene

metG

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.UniRule annotation

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei303ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligaseUniRule annotation (EC:6.1.1.10UniRule annotation)
Alternative name(s):
Methionyl-tRNA synthetaseUniRule annotation
Short name:
MetRSUniRule annotation
Gene namesi
Name:metGUniRule annotation
Ordered Locus Names:BR0995, BS1330_I0991
OrganismiBrucella suis biovar 1 (strain 1330)
Taxonomic identifieri204722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000007104 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001392131 – 515Methionine--tRNA ligaseAdd BLAST515

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

Secondary structure

1515
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 10Combined sources6
Helixi21 – 39Combined sources19
Beta strandi43 – 50Combined sources8
Helixi55 – 64Combined sources10
Helixi68 – 85Combined sources18
Beta strandi91 – 95Combined sources5
Helixi99 – 114Combined sources16
Beta strandi118 – 122Combined sources5
Beta strandi125 – 128Combined sources4
Turni129 – 132Combined sources4
Beta strandi133 – 135Combined sources3
Helixi137 – 139Combined sources3
Beta strandi153 – 155Combined sources3
Beta strandi157 – 159Combined sources3
Beta strandi162 – 166Combined sources5
Helixi168 – 171Combined sources4
Helixi172 – 181Combined sources10
Beta strandi185 – 188Combined sources4
Helixi189 – 200Combined sources12
Beta strandi209 – 212Combined sources4
Beta strandi214 – 216Combined sources3
Beta strandi222 – 227Combined sources6
Helixi229 – 234Combined sources6
Helixi236 – 239Combined sources4
Turni240 – 244Combined sources5
Helixi249 – 253Combined sources5
Beta strandi257 – 262Combined sources6
Helixi263 – 265Combined sources3
Helixi266 – 270Combined sources5
Helixi272 – 279Combined sources8
Beta strandi288 – 291Combined sources4
Beta strandi294 – 296Combined sources3
Helixi311 – 318Combined sources8
Helixi320 – 330Combined sources11
Beta strandi337 – 339Combined sources3
Helixi341 – 351Combined sources11
Turni352 – 355Combined sources4
Helixi356 – 370Combined sources15
Helixi383 – 404Combined sources22
Helixi408 – 429Combined sources22
Helixi431 – 437Combined sources7
Helixi439 – 460Combined sources22
Turni461 – 463Combined sources3
Helixi465 – 474Combined sources10
Helixi479 – 481Combined sources3
Helixi486 – 489Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5K0SX-ray2.45A/B/C1-515[»]
5K0TX-ray2.60A/B/C1-515[»]
ProteinModelPortaliP59078.
SMRiP59078.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi13 – 23"HIGH" regionAdd BLAST11
Motifi300 – 304"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000200401.
KOiK01874.
OMAiHTFYVWF.

Family and domain databases

CDDicd00814. MetRS_core. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_01228. Met_tRNA_synth_type2. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR033911. MetRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59078-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSREKYYITT AIAYPNGKPH IGHAYELIAT DAMARFQRLN GMDVYFLTGT
60 70 80 90 100
DEHGIKMLQS ARKEGITPRE LADRNTSAFR RMAEVLNSSN DDYIRTSEER
110 120 130 140 150
HYKASQAIWQ AMVANGDIYK GGYAGWYSVR DEAYYGEEET EVRADGVRYG
160 170 180 190 200
PQGTPVEWVE EESYFFRLSA YQDKLLDLYE NNPGFIMPAE RRNEIVSFVK
210 220 230 240 250
SGLKDLSISR TTFDWGIPVP GDEKHVMYVW VDALTNYITA LGYPDTTDER
260 270 280 290 300
WAYWPANAHI IGKDISRFHA VYWPAFLMSA QLPLPKRVFA HGFLFNRGEK
310 320 330 340 350
MSKSVGNVID PFELVERYGL DQLRYFLMRE VPFGQDGSYS HEAIVNRTNA
360 370 380 390 400
DLANDLGNLA QRSLSMIAKN CEGKVPQPGA FSEADKAILD QADAALETAR
410 420 430 440 450
KAMDDQALHL ALGAIFAVVA EANRYFAGQE PWALRKTDPA RMGTVLYVTA
460 470 480 490 500
EVLRRVGIMV QPFIPQSAEK LLDILAVPAD KRQFADVLAS PLAGGTDLPA
510
PQPVFPRYVE ADEQN
Length:515
Mass (Da):57,998
Last modified:November 8, 2002 - v1
Checksum:iB3CBD8FA93515199
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN29918.1.
CP002997 Genomic DNA. Translation: AEM18335.1.
RefSeqiWP_004689678.1. NZ_KN046804.1.

Genome annotation databases

EnsemblBacteriaiAAN29918; AAN29918; BR0995.
AEM18335; AEM18335; BS1330_I0991.
KEGGibms:BR0995.
bsi:BS1330_I0991.
PATRICi17790302. VBIBruSui107850_1007.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014291 Genomic DNA. Translation: AAN29918.1.
CP002997 Genomic DNA. Translation: AEM18335.1.
RefSeqiWP_004689678.1. NZ_KN046804.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5K0SX-ray2.45A/B/C1-515[»]
5K0TX-ray2.60A/B/C1-515[»]
ProteinModelPortaliP59078.
SMRiP59078.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN29918; AAN29918; BR0995.
AEM18335; AEM18335; BS1330_I0991.
KEGGibms:BR0995.
bsi:BS1330_I0991.
PATRICi17790302. VBIBruSui107850_1007.

Phylogenomic databases

HOGENOMiHOG000200401.
KOiK01874.
OMAiHTFYVWF.

Family and domain databases

CDDicd00814. MetRS_core. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_01228. Met_tRNA_synth_type2. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR033911. MetRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYM_BRUSU
AccessioniPrimary (citable) accession number: P59078
Secondary accession number(s): G0K9R9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: November 8, 2002
Last modified: November 30, 2016
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Brucella suis
    Brucella suis (strain 1330): entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.