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Reviewed, UniProtKB/Swiss-Prot P59078 (SYM_BRUSU)

Last modified November 3, 2009. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS
Gene names
Name: metG
Ordered Locus Names: BR0995
OrganismBrucella suis [Complete proteome] [HAMAP]
Taxonomic identifier29461 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length515 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_01228

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm. HAMAP MF_01228

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processmethionyl-tRNA aminoacylation

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: HAMAP

methionine-tRNA ligase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 515515Methionyl-tRNA synthetase HAMAP MF_01228
PRO_0000139213

Regions

Motif13 – 2311"HIGH" region HAMAP MF_01228
Motif300 – 3045"KMSKS" region HAMAP MF_01228

Sites

Binding site3031ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P59078-1 [UniParc].

Last modified November 8, 2002. Version 1.
Checksum: B3CBD8FA93515199

FASTA51557,998
        10         20         30         40         50         60 
MSREKYYITT AIAYPNGKPH IGHAYELIAT DAMARFQRLN GMDVYFLTGT DEHGIKMLQS 

        70         80         90        100        110        120 
ARKEGITPRE LADRNTSAFR RMAEVLNSSN DDYIRTSEER HYKASQAIWQ AMVANGDIYK 

       130        140        150        160        170        180 
GGYAGWYSVR DEAYYGEEET EVRADGVRYG PQGTPVEWVE EESYFFRLSA YQDKLLDLYE 

       190        200        210        220        230        240 
NNPGFIMPAE RRNEIVSFVK SGLKDLSISR TTFDWGIPVP GDEKHVMYVW VDALTNYITA 

       250        260        270        280        290        300 
LGYPDTTDER WAYWPANAHI IGKDISRFHA VYWPAFLMSA QLPLPKRVFA HGFLFNRGEK 

       310        320        330        340        350        360 
MSKSVGNVID PFELVERYGL DQLRYFLMRE VPFGQDGSYS HEAIVNRTNA DLANDLGNLA 

       370        380        390        400        410        420 
QRSLSMIAKN CEGKVPQPGA FSEADKAILD QADAALETAR KAMDDQALHL ALGAIFAVVA 

       430        440        450        460        470        480 
EANRYFAGQE PWALRKTDPA RMGTVLYVTA EVLRRVGIMV QPFIPQSAEK LLDILAVPAD 

       490        500        510 
KRQFADVLAS PLAGGTDLPA PQPVFPRYVE ADEQN 

« Hide

Cross-references

Sequence databases

AE014291 Genomic DNA. Translation: AAN29918.1.
RefSeqNP_698003.1.

3D structure databases

HSSPHSSP built from PDB template 1A8H based on UniProtKB P23395.
ModBaseSearch...

Genome annotation databases

GeneID1166668.
GenomeReviewsGene locus BR0995 in contig AE014291_GR.
KEGGbms:BR0995.
NMPDRfig|204722.1.peg.960.
TIGRBR0995.

Phylogenomic databases

HOGENOMP59078.
OMAKMSKYAD.

Enzyme and pathway databases

BioCycBSUI204722:BR_0995-MON.
BRENDA6.1.1.10. 281610.

Family and domain databases

HAMAPMF_01228.
[Tree]
InterProIPR015413. aa-tRNA-synt_I.
IPR001412. aa-tRNA-synth_I_CS.
IPR002304. Met-tRNA-synth_Ia.
IPR014729. Rossmann-like_a/b/a_fold.
IPR014758. tRNA-synt_met_N.
[Graphical view]
Gene3DG3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PfamPF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
TIGRFAMsTIGR00398. metG. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYM_BRUSU
AccessionPrimary (citable) accession number: P59078
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: November 8, 2002
Last modified: November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

Brucella suis

Brucella suis (strain 1330): entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents