P59076 (SYM_BIFLO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine--tRNA ligase
EC=6.1.1.10

Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	BL0170

Organism

Bifidobacterium longum (strain NCC 2705)

Taxonomic identifier

206672 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	621 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_00098
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00098
Subunit structure	Monomer By similarity. HAMAP MF_00098
Subcellular location	Cytoplasm HAMAP MF_00098.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 621	621	Methionine--tRNA ligase HAMAP MF_00098		PRO_0000139105
Regions
	Motif	11 – 21	11	"HIGH" region HAMAP MF_00098
	Motif	347 – 351	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	143	1	Zinc By similarity
	Metal binding	146	1	Zinc By similarity
	Metal binding	156	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity
	Binding site	350	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P59076 [UniParc]. Last modified November 8, 2002. Version 1. Checksum: 71227BA1B73C0E18	FASTA	621	69,091
	10 20 30 40 50 60 MSHVLVNVAW PYANGPRHIG HVAGFGVPSD VYARYERMKG NDVLMVSGTD EHGTPILVEA 70 80 90 100 110 120 EKEGLTAQEL ANRYNRVIAK DLCDLGLSYD LFTRTTTGNH EHVVQEMFKQ CLENGYIYKG 130 140 150 160 170 180 TQQVAISPST GRTLPDRYIE GECPICHAEG ARGDQCDACG NELDPDELIN PVSKINGETP 190 200 210 220 230 240 RFEQTEHYFL DLPALAEANK AWLETRKGWR TNVINFSLGL FKEVKPRAIT RDIDWGIPVP 250 260 270 280 290 300 VKGWIDNPNK KLYVWFDAVI GYLSASIEWA RRQGDPEKWR EWWNDPACPA YYFMGKDNIT 310 320 330 340 350 360 FHSQIWPSEM LAYNGKGSKG GETGPMGPLN LPEQVVASEF MTMEGKKFSS SRGIVIYVKD 370 380 390 400 410 420 ILARYPVDAV RYYISVAGPE SSDSDFTWAE FVRHNNEELA SSWGNLVNRV ANLINKNFGE 430 440 450 460 470 480 IPPLDEDSMT NEDRGLLEEA SAAFDVVGSS IETHHQKHAL SEAMRVVGDI NKYISATEPW 490 500 510 520 530 540 KIKDDQARLG TVLHVAAQAV SDANHLLAPF LPHSAQKVWE ALGGTGTFSP LPELKEVEDL 550 560 570 580 590 600 DKPGFTYPII TGDYELGVNV HPWKSEAIEV GAMVPKPAPI FAKIPTEAVE EELARFDEAL 610 620 AARRAAEAER LAAEKAKLAA E

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014295 Genomic DNA. Translation: AAN24025.1.
RefSeq	NP_695389.1. NC_004307.2.
3D structure databases
ProteinModelPortal	P59076.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1022616.
GenomeReviews	Gene locus BL0170 in contig AE014295_GR.
KEGG	blo:BL0170.
NMPDR	fig|206672.1.peg.158.
PATRIC	21120068. VBIBifLon107831_1470.
Phylogenomic databases
HOGENOM	HBG721667.
OMA	FKQCLEN.
PhylomeDB	P59076.
ProtClustDB	PRK12268.
Enzyme and pathway databases
BioCyc	BLON206672:BL0170-MONOMER.
Family and domain databases
HAMAP	MF_00098. Met_tRNA_synth_type1. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR014758. Met-tRNA_synth. IPR023458. Met-tRNA_synth_1. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01874.
Pfam	PF08264. Anticodon_1. 1 hit. PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00398. MetG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_BIFLO

Accession

Primary (citable) accession number: P59076

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	November 8, 2002
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program