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Protein

Basic phospholipase A2 VRV-PL-VIIIa

Gene
N/A
Organism
Daboia russelii (Russel's viper) (Vipera russelii)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Snake venom phospholipase A2 (PLA2) that shows weak neurotoxicity and medium anticoagulant effects by binding to factor Xa (F10) and inhibiting the prothrombinase activity (IC50 is 130 nM) (PubMed:18062812). It also damages vital organs such as lung, liver and kidney, displays edema-inducing activities when injected into the foot pads of mice and induces necrosis of muscle cells when injected into the thigh muscle. Has a low enzymatic activity. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.3 Publications

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation

Cofactori

Ca2+1 PublicationNote: Binds 1 Ca2+ ion.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi27Calcium; via carbonyl oxygenCombined sources1 Publication1
Metal bindingi29Calcium; via carbonyl oxygenCombined sources1 Publication1
Metal bindingi31Calcium; via carbonyl oxygenCombined sources1 Publication1
Active sitei47By similarity1
Metal bindingi48CalciumCombined sources1 Publication1
Active sitei89By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Blood coagulation cascade inhibiting toxin, Hemostasis impairing toxin, Hydrolase, Myotoxin, Neurotoxin, Presynaptic neurotoxin, Toxin

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.1.4. 5485.

Names & Taxonomyi

Protein namesi
Recommended name:
Basic phospholipase A2 VRV-PL-VIIIa (EC:3.1.1.4)
Short name:
svPLA2
Alternative name(s):
DPLA2
P1
Phosphatidylcholine 2-acylhydrolase
Phospholipase A2 4
Short name:
PLA24
OrganismiDaboia russelii (Russel's viper) (Vipera russelii)
Taxonomic identifieri31159 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeViperinaeDaboia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is between 5.3 and 5.5 mg/kg by intravenous injection into mice.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001617171 – 121Basic phospholipase A2 VRV-PL-VIIIaAdd BLAST121

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi26 ↔ 115Combined sources3 Publications
Disulfide bondi28 ↔ 44Combined sources3 Publications
Disulfide bondi43 ↔ 95Combined sources3 Publications
Disulfide bondi49 ↔ 121Combined sources3 Publications
Disulfide bondi50 ↔ 88Combined sources3 Publications
Disulfide bondi57 ↔ 81Combined sources3 Publications
Disulfide bondi75 ↔ 86Combined sources3 Publications

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

Secondary structure

1121
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 13Combined sources12
Helixi17 – 20Combined sources4
Beta strandi22 – 24Combined sources3
Turni25 – 27Combined sources3
Beta strandi28 – 30Combined sources3
Beta strandi32 – 34Combined sources3
Helixi39 – 52Combined sources14
Beta strandi55 – 57Combined sources3
Turni59 – 61Combined sources3
Beta strandi66 – 69Combined sources4
Beta strandi72 – 75Combined sources4
Helixi80 – 98Combined sources19
Helixi99 – 102Combined sources4
Helixi105 – 107Combined sources3
Helixi112 – 114Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CL5X-ray2.45A/B1-38[»]
1FB2X-ray1.95A/B1-49[»]
1FV0X-ray1.70A/B1-49[»]
1JQ8X-ray2.00A/B1-121[»]
1JQ9X-ray1.80A/B1-121[»]
1KPMX-ray1.80A/B1-50[»]
1OXLX-ray1.80A/B1-111[»]
1OYFX-ray2.45A1-121[»]
1SKGX-ray1.21A1-121[»]
1SQZX-ray1.20A1-111[»]
1SV3X-ray1.35A1-50[»]
1SV9X-ray2.71A1-111[»]
1SXKX-ray1.21A1-111[»]
1TDVX-ray1.70A1-121[»]
1TG1X-ray1.25A1-121[»]
1TG4X-ray1.70A1-121[»]
1TGMX-ray1.86A1-121[»]
1TH6X-ray1.23A1-121[»]
1TJ9X-ray1.10A1-121[»]
1TJKX-ray1.25A1-121[»]
1TK4X-ray1.10A1-111[»]
1TP2X-ray2.40A/B1-111[»]
1Y38X-ray2.44A/B1-121[»]
1ZR8X-ray2.03A1-111[»]
1ZWPX-ray1.10A1-111[»]
1ZYXX-ray1.95A1-121[»]
2B17X-ray2.71A1-121[»]
2DO2X-ray2.60A1-121[»]
2DPZX-ray2.10A1-121[»]
2FNXX-ray2.70A1-111[»]
2G58X-ray0.98A1-121[»]
2GNSX-ray2.30A1-121[»]
2O1NX-ray2.80A1-121[»]
2OLIX-ray2.21A1-121[»]
2OTFX-ray1.95A1-121[»]
2OTHX-ray2.90A1-121[»]
2OUBX-ray2.75A1-121[»]
2OYFX-ray1.20A1-121[»]
2PB8X-ray2.00A1-121[»]
2PMJX-ray2.40A1-121[»]
2PVTX-ray2.10A1-117[»]
2PWSX-ray2.21A1-121[»]
2PYCX-ray1.50A1-121[»]
2Q1PX-ray1.50A1-121[»]
2QHWX-ray2.21A1-121[»]
2QU9X-ray2.08A1-121[»]
2QUEX-ray2.25A1-121[»]
2QVDX-ray1.93A1-121[»]
2ZBHX-ray2.60A1-121[»]
3CBIX-ray3.15A/B/C/D1-121[»]
3G8FX-ray1.25A1-121[»]
3H1XX-ray1.40A1-121[»]
ProteinModelPortaliP59071.
SMRiP59071.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP59071.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG008137.

Family and domain databases

CDDicd00125. PLA2c. 1 hit.
Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59071-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
SLLEFGKMIL EETGKLAIPS YSSYGCYCGW GGKGTPKDAT DRCCFVHDCC
60 70 80 90 100
YGNLPDCNPK SDRYKYKRVN GAIVCEKGTS CENRICECDK AAAICFRQNL
110 120
NTYSKKYMLY PDFLCKGELK C
Length:121
Mass (Da):13,611
Last modified:November 1, 2002 - v1
Checksum:i5CDF82DDA7DA638E
GO

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CL5X-ray2.45A/B1-38[»]
1FB2X-ray1.95A/B1-49[»]
1FV0X-ray1.70A/B1-49[»]
1JQ8X-ray2.00A/B1-121[»]
1JQ9X-ray1.80A/B1-121[»]
1KPMX-ray1.80A/B1-50[»]
1OXLX-ray1.80A/B1-111[»]
1OYFX-ray2.45A1-121[»]
1SKGX-ray1.21A1-121[»]
1SQZX-ray1.20A1-111[»]
1SV3X-ray1.35A1-50[»]
1SV9X-ray2.71A1-111[»]
1SXKX-ray1.21A1-111[»]
1TDVX-ray1.70A1-121[»]
1TG1X-ray1.25A1-121[»]
1TG4X-ray1.70A1-121[»]
1TGMX-ray1.86A1-121[»]
1TH6X-ray1.23A1-121[»]
1TJ9X-ray1.10A1-121[»]
1TJKX-ray1.25A1-121[»]
1TK4X-ray1.10A1-111[»]
1TP2X-ray2.40A/B1-111[»]
1Y38X-ray2.44A/B1-121[»]
1ZR8X-ray2.03A1-111[»]
1ZWPX-ray1.10A1-111[»]
1ZYXX-ray1.95A1-121[»]
2B17X-ray2.71A1-121[»]
2DO2X-ray2.60A1-121[»]
2DPZX-ray2.10A1-121[»]
2FNXX-ray2.70A1-111[»]
2G58X-ray0.98A1-121[»]
2GNSX-ray2.30A1-121[»]
2O1NX-ray2.80A1-121[»]
2OLIX-ray2.21A1-121[»]
2OTFX-ray1.95A1-121[»]
2OTHX-ray2.90A1-121[»]
2OUBX-ray2.75A1-121[»]
2OYFX-ray1.20A1-121[»]
2PB8X-ray2.00A1-121[»]
2PMJX-ray2.40A1-121[»]
2PVTX-ray2.10A1-117[»]
2PWSX-ray2.21A1-121[»]
2PYCX-ray1.50A1-121[»]
2Q1PX-ray1.50A1-121[»]
2QHWX-ray2.21A1-121[»]
2QU9X-ray2.08A1-121[»]
2QUEX-ray2.25A1-121[»]
2QVDX-ray1.93A1-121[»]
2ZBHX-ray2.60A1-121[»]
3CBIX-ray3.15A/B/C/D1-121[»]
3G8FX-ray1.25A1-121[»]
3H1XX-ray1.40A1-121[»]
ProteinModelPortaliP59071.
SMRiP59071.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG008137.

Enzyme and pathway databases

BRENDAi3.1.1.4. 5485.

Miscellaneous databases

EvolutionaryTraceiP59071.

Family and domain databases

CDDicd00125. PLA2c. 1 hit.
Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPA2B8_DABRR
AccessioniPrimary (citable) accession number: P59071
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: November 1, 2002
Last modified: November 30, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.