P59056 (SYFA_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha subunit
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha subunit
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	BUsg_121

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP-Rule MF_00281
Cofactor	Binds 2 magnesium ions per tetramer Potential.
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 1 subfamily.
Caution	Lacks the conserved glutamate residue that binds magnesium.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	Phenylalanine--tRNA ligase alpha subunit HAMAP-Rule MF_00281		PRO_0000126677

Sequences

	Sequence		Length	Mass (Da)
	P59056 [UniParc]. Last modified October 25, 2002. Version 1. Checksum: BD51678030D4E6D4	FASTA	329	38,862
	10 20 30 40 50 60 MLILQKLFDS VKIEIKNSHT IDKLDHIRIK YLGKKGIFSN LIKDLKNFSL EERKKYFIIF 70 80 90 100 110 120 NEIKKKTINQ INKKKIELNK IILDKQIEKE KIDVSLPGRR IKNGVIHPIN HTITYIKNFF 130 140 150 160 170 180 SKLGFESISG FEIEDEYHNF DALNIPKDHP ARNIHDTFWF DENRLLRTQT SSMQIRIMKK 190 200 210 220 230 240 EKPPIRLIFP GKVYRNDYDS THTPMFHQVE GLIVEKNINF SNLKWIIYNF LRNFFHKDIA 250 260 270 280 290 300 IRFRPSYFPF TTPSAEVDVV TNNGKLLEVL GCGMVHPSVL KNVNIDSNFY SACAFGIGIE 310 320 RITMLRYGIS DLRSFFENDI RFIKQFKHI

References

[1]

"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE013218 Genomic DNA. Translation: AAM67689.1.
RefSeq	NP_660478.1. NC_004061.1.
3D structure databases
ProteinModelPortal	P59056.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg121.
Proteomic databases
PRIDE	P59056.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM67689; AAM67689; BUsg_121.
GeneID	1005938.
KEGG	bas:BUsg121.
PATRIC	21247023. VBIBucAph100086_0125.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0016.
KO	K01889.
OMA	LTHTPMF.
ProtClustDB	PRK00488.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-131-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1.
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_ligase_II_N. IPR022911. Phe_tRNA_ligase_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_BUCAP

Accession

Primary (citable) accession number: P59056

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	October 25, 2002
Last modified:	May 29, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program