Reviewed, UniProtKB/Swiss-Prot P59050 (LDH1_BIFLO)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase 1
      Short name=L-LDH 1
    EC=1.1.1.27

Gene names

Name:	ldh1
Ordered Locus Names:	BL0710

Organism

Bifidobacterium longum [Complete proteome] [HAMAP]

Taxonomic identifier

216816 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Bifidobacteriales › Bifidobacteriaceae › Bifidobacterium

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-lactate + NAD(+) = pyruvate + NADH.
Pathway	Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the LDH/MDH superfamily. LDH family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	anaerobic glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	L-lactate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	L-lactate dehydrogenase 1		PRO_0000168330
Regions
	Nucleotide binding	15 – 43	29	NAD By similarity
Sites
	Active site	179	1	Proton acceptor By similarity
	Binding site	92	1	Substrate By similarity
	Binding site	124	1	NAD or substrate By similarity
	Binding site	155	1	Substrate By similarity
	Binding site	234	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P59050-1 [UniParc]. Last modified October 19, 2002. Version 1. Checksum: E7A68E9A00992108	FASTA	316	33,871
	10 20 30 40 50 60 MVTMNRNKVV IVGTGQVGAT AAFGIVTHGL CNELVLIDCS AAKALGEARD LDDGSEFQDR 70 80 90 100 110 120 HVKVRAGDYA DCKDADIVVI TVGRKPPANS NRMAELGFTV GLVGEVVDNV MASGFDGVIV 130 140 150 160 170 180 MVSNPVDVMA WYAWKRSGLP RTQVLGTGTA LDTSRLKTII GEETGLDPRN VGGFVMGEHG 190 200 210 220 230 240 DSQFTAWSTV SLGGKPFARF LADNQDRFAS VSTTEIEEKT RTRGNEIVAA KGGTNFGIAS 250 260 270 280 290 300 TVAGIVQTIL WDERRIVPVS TLLDGEYGEH DVFLGVPTEL RANGANEIVE LDLSEDERAK 310 LHHSAELVRE HCEGLL

References

[1]

"The genome sequence of Bifidobacterium longum reflects its adaptation to the human gastrointestinal tract." Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G., Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F. Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002) [PubMed: 12381787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCC 2705.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014295 Genomic DNA. Translation: AAN24529.1.
RefSeq	NP_695893.1.
3D structure databases
HSSP	HSSP built from PDB template 2LDB based on UniProtKB P00344.
ModBase	Search...
Genome annotation databases
GeneID	1023257.
GenomeReviews	Gene locus BL0710 in contig AE014295_GR.
KEGG	blo:BL0710.
NMPDR	fig|206672.1.peg.660.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P59050.
Enzyme and pathway databases
BioCyc	BLON206672:BL0710-MON.
Family and domain databases
HAMAP	MF_00488. [Tree]
InterPro	IPR001557. L-lactate/malate_DHase. IPR011304. L-lactate_DHase. IPR001236. Lactate/malate_DHase. IPR015955. Lactate_DHase/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
TIGRFAMs	TIGR01771. L-LDH-NAD. 1 hit.
PROSITE	PS00064. L_LDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDH1_BIFLO

Accession

Primary (citable) accession number: P59050

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	October 19, 2002
Last modified:	November 25, 2008
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)