Reviewed, UniProtKB/Swiss-Prot P59028 (LEU3_CHLTE)

Names and origin

Protein names

Recommended name:
    3-isopropylmalate dehydrogenase
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase
      Short name=IMDH
    3-IPM-DH

Gene names

Name:	leuB
Ordered Locus Names:	CT0615

Organism

Chlorobium tepidum [Complete proteome] [HAMAP]

Taxonomic identifier

1097 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
Catalytic activity	(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.
Subunit structure	Homodimer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	3-isopropylmalate dehydrogenase		PRO_0000083678
Regions
	Nucleotide binding	76 – 89	14	NAD By similarity
	Nucleotide binding	277 – 289	13	NAD By similarity
Sites
	Metal binding	220	1	Magnesium or manganese By similarity
	Metal binding	244	1	Magnesium or manganese By similarity
	Metal binding	248	1	Magnesium or manganese By similarity
	Binding site	96	1	Substrate By similarity
	Binding site	106	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	220	1	Substrate By similarity
	Site	141	1	Important for catalysis By similarity
	Site	188	1	Important for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P59028-1 [UniParc]. Last modified October 19, 2002. Version 1. Checksum: 28F202BC704BB3A0	FASTA	352	38,169
	10 20 30 40 50 60 MMYKIVSIPG DGIGPEVVAG ALDVLNAVAK KHGFEVSVEE HLFGGASYDV HGSMLTDETL 70 80 90 100 110 120 EACKNCDAVL LGAVGGYKWE NLPHDKKPEA ALLKIRKELG LFANLRPARV YDALVASSTL 130 140 150 160 170 180 KTEVVQGTDF MVFRELTGGI YFGQPRGYDE TRGWNTMVYE RYEVERIARL AFEYAQKRGN 190 200 210 220 230 240 AKVTSIDKAN VLEVSQFWRN IVHEVHQDFP EIELVDMYVD NAAMQVVRNP KQFEVIVTSN 250 260 270 280 290 300 LFGDILSDIS GMITGSLGML PSASIGSEHA LYEPIHGSAP DIAGQNKANP IATIASVAMM 310 320 330 340 350 FENSFNRPEV AADIYAAIEG ALAAGFRTGD IAAAGEAISS TTEMTAAIVA RI

References

[1]

"The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium." Eisen J.A., Nelson K.E., Paulsen I.T., Heidelberg J.F., Wu M., Dodson R.J., DeBoy R.T., Gwinn M.L., Nelson W.C., Haft D.H., Hickey E.K., Peterson J.D., Durkin A.S., Kolonay J.F., Yang F., Holt I.E., Umayam L.A., Mason T.M. , Brenner M., Shea T.P., Parksey D.S., Nierman W.C., Feldblyum T.V., Hansen C.L., Craven M.B., Radune D., Vamathevan J.J., Khouri H.M., White O., Gruber T.M., Ketchum K.A., Venter J.C., Tettelin H., Bryant D.A., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:9509-9514(2002) [PubMed: 12093901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 49652 / DSM 12025 / TLS.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE006470 Genomic DNA. Translation: AAM71857.1.
RefSeq	NP_661515.1.
3D structure databases
HSSP	HSSP built from PDB template 1CM7 based on UniProtKB P30125.
ModBase	Search...
Genome annotation databases
GeneID	1006259.
GenomeReviews	Gene locus CT0615 in contig AE006470_GR.
KEGG	cte:CT0615.
NMPDR	fig|194439.1.peg.609.
TIGR	CT0615.
Phylogenomic databases
HOGENOM	P59028.
Enzyme and pathway databases
BioCyc	CTEP194439:CT_0615-MON.
Family and domain databases
HAMAP	MF_01033. [Tree]
InterPro	IPR004429. 3-isopropylmalate_DHase. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00169. leuB. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU3_CHLTE

Accession

Primary (citable) accession number: P59028

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	October 19, 2002
Last modified:	November 4, 2008
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)