Skip Header

Contribute Send feedback
Read comments (?) or add your own

P59028 (LEU3_CHLTE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3-isopropylmalate dehydrogenase
EC=1.1.1.85
Alternative name(s):
3-IPM-DH
Beta-IPM dehydrogenase
Short name=IMDH
Gene names
Name:leuB
Ordered Locus Names:CT0615
OrganismChlorobium tepidum
Taxonomic identifier1097 [NCBI]
Taxonomic lineageBacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobaculum

Protein attributes

Sequence length352 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. HAMAP MF_01033

Catalytic activity

(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH. HAMAP MF_01033

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. HAMAP MF_01033

Subunit structure

Homodimer By similarity. HAMAP MF_01033

Subcellular location

Cytoplasm By similarity HAMAP MF_01033.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3523523-isopropylmalate dehydrogenase HAMAP MF_01033
PRO_0000083678

Regions

Nucleotide binding76 – 8914NAD By similarity
Nucleotide binding277 – 28913NAD By similarity

Sites

Metal binding2201Magnesium or manganese By similarity
Metal binding2441Magnesium or manganese By similarity
Metal binding2481Magnesium or manganese By similarity
Binding site961Substrate By similarity
Binding site1061Substrate By similarity
Binding site1341Substrate By similarity
Binding site2201Substrate By similarity
Site1411Important for catalysis By similarity
Site1881Important for catalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
P59028 [UniParc].

Last modified October 19, 2002. Version 1.
Checksum: 28F202BC704BB3A0

FASTA35238,169
        10         20         30         40         50         60 
MMYKIVSIPG DGIGPEVVAG ALDVLNAVAK KHGFEVSVEE HLFGGASYDV HGSMLTDETL 

        70         80         90        100        110        120 
EACKNCDAVL LGAVGGYKWE NLPHDKKPEA ALLKIRKELG LFANLRPARV YDALVASSTL 

       130        140        150        160        170        180 
KTEVVQGTDF MVFRELTGGI YFGQPRGYDE TRGWNTMVYE RYEVERIARL AFEYAQKRGN 

       190        200        210        220        230        240 
AKVTSIDKAN VLEVSQFWRN IVHEVHQDFP EIELVDMYVD NAAMQVVRNP KQFEVIVTSN 

       250        260        270        280        290        300 
LFGDILSDIS GMITGSLGML PSASIGSEHA LYEPIHGSAP DIAGQNKANP IATIASVAMM 

       310        320        330        340        350 
FENSFNRPEV AADIYAAIEG ALAAGFRTGD IAAAGEAISS TTEMTAAIVA RI

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE006470 Genomic DNA. Translation: AAM71857.1.
RefSeqNP_661515.1. NC_002932.3.

3D structure databases

ProteinModelPortalP59028.
SMRP59028. Positions 3-352.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1006259.
GenomeReviewsGene locus CT0615 in contig AE006470_GR.
KEGGcte:CT0615.
NMPDRfig|194439.1.peg.609.
PATRIC21399297. VBIChlTep116050_0572.
TIGRCT0615.

Phylogenomic databases

HOGENOMHBG518924.
OMAAAMMLRF.
ProtClustDBPRK00772.

Enzyme and pathway databases

BioCycCTEP194439:CT_0615-MONOMER.

Family and domain databases

HAMAPMF_01033. LeuB_type1.
[Tree]
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR024084. IsoPropMal-DH-like_dom.
IPR004429. Isopropylmalate_DH.
[Graphical view]
Gene3DG3DSA:3.40.718.10. IDH_IMDH. 1 hit.
KOK00052.
PANTHERPTHR11835. IDH_IMDH_dimeric. 1 hit.
PTHR11835:SF13. IPMDH. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsTIGR00169. LeuB. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEU3_CHLTE
AccessionPrimary (citable) accession number: P59028
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: October 19, 2002
Last modified: January 25, 2012
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents