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P59027 (LEU3_BUCPS) Reviewed, UniProtKB/Swiss-Prot

Last modified July 27, 2011. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
3-isopropylmalate dehydrogenase
EC=1.1.1.85
Alternative name(s):
3-IPM-DH
Beta-IPM dehydrogenase
Short name=IMDH
Gene names
Name:leuB
OrganismBuchnera aphidicola subsp. Pemphigus spyrothecae
Taxonomic identifier98799 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length363 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. HAMAP MF_01033

Catalytic activity

(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH. HAMAP MF_01033

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. HAMAP MF_01033

Subunit structure

Homodimer By similarity. HAMAP MF_01033

Subcellular location

Cytoplasm By similarity HAMAP MF_01033.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3633633-isopropylmalate dehydrogenase HAMAP MF_01033
PRO_0000083659

Regions

Nucleotide binding77 – 9014NAD By similarity
Nucleotide binding284 – 29613NAD By similarity

Sites

Metal binding2261Magnesium or manganese By similarity
Metal binding2501Magnesium or manganese By similarity
Metal binding2541Magnesium or manganese By similarity
Binding site981Substrate By similarity
Binding site1081Substrate By similarity
Binding site1371Substrate By similarity
Binding site2261Substrate By similarity
Site1441Important for catalysis By similarity
Site1941Important for catalysis By similarity

Sequences

Sequence LengthMass (Da)Tools
P59027 [UniParc].

Last modified October 19, 2002. Version 1.
Checksum: 760AAA94425D6632

FASTA36340,550
        10         20         30         40         50         60 
MTKIYNLAIL PGDGIGPELL QKDLNFKVLK EQYKLKINTT EYDIGGIAID KYGKALPKET 

        70         80         90        100        110        120 
LDGCKESDSI LFGSVGGPKW QHLPPDQQPE RAALLPIRKY FNLFSNLRPA KLYSTLNHLS 

       130        140        150        160        170        180 
PLRRDISEKG FDILCVRELT GGIYFGKPKG YIKNIIDKKA FDTEIYYASE IKRIAHIGFK 

       190        200        210        220        230        240 
LAQNRKYKIT SVDKANVLES SVLWRETVNT ISKEYPDVKL SHLYIDHAAM QIIKNPDKFD 

       250        260        270        280        290        300 
VILTSNLFGD ILSDECAMIT GSIGMLPSAS LNDSNFGLYE PAGGSAPDIA GKNIANPIAQ 

       310        320        330        340        350        360 
ILSIAMLVRY TMKLPMISDH IESAVIDTLK KGYRTLDIAN DKEKYVSTND IGDIISDILK 


KRI

« Hide

References

[1]"Molecular characterization of the leucine cluster in Buchnera sp. strain PSY, a primary endosymbiont of the aphid Pemphigus spyrothecae."
Sabater-Munoz B., Gomez-Valero L., van Ham R.C.H.J., Silva F.J., Latorre A.
Appl. Environ. Microbiol. 68:2572-2575(2002) [PubMed: 11976137] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AJ426489 Genomic DNA. Translation: CAD20138.1.

3D structure databases

ProteinModelPortalP59027.
SMRP59027. Positions 1-356.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

HAMAPMF_01033. LeuB_type1.
[Tree]
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR001804. Isocitrate/isopropylmalate_DH.
IPR024084. IsoPropMal-DH-like_dom.
IPR004429. Isopropylmalate_DH.
[Graphical view]
Gene3DG3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHERPTHR11835. IDH_IMDH_dimeric. 1 hit.
PTHR11835:SF13. IPMDH. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsTIGR00169. LeuB. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEU3_BUCPS
AccessionPrimary (citable) accession number: P59027
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: October 19, 2002
Last modified: July 27, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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