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Protein

Vacuolar protein sorting-associated protein 33B

Gene

Vps33b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Mediates phagolysosomal fusion in macrophages. Proposed to be involved in endosomal maturation implicating in part VIPAS39 (By similarity). In epithelial cells, the VPS33B:VIPAS39 complex may play a role in the apical RAB11A-dependentrecycling pathway and in the maintenance of the apical-basolateral polarity (PubMed:20190753). Seems to be involved in the sorting of specific cargos from the trans-Golgi network to alpha-granule-destined multivesicular bodies (MVBs) promoting MVBs maturation in megakaryocytes (PubMed:25947942).By similarity2 Publications

GO - Biological processi

  • endosome organization Source: MGI
  • lysosome localization Source: MGI
  • megakaryocyte development Source: UniProtKB
  • melanosome localization Source: MGI
  • membrane fusion Source: MGI
  • platelet alpha granule organization Source: UniProtKB
  • protein transport Source: MGI
  • regulation of platelet aggregation Source: UniProtKB
  • vesicle docking involved in exocytosis Source: InterPro
  • vesicle-mediated transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 33B
Gene namesi
Name:Vps33b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2446237. Vps33b.

Subcellular locationi

  • Late endosome membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
  • Lysosome membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
  • Early endosome By similarity
  • Cytoplasmic vesicleclathrin-coated vesicle By similarity
  • Recycling endosome By similarity

  • Note: Colocalizes in clusters with VIPAS39 at cytoplasmic organelles. Colocalizes with RAB11A and VIPAS39 on recycling endosomes. Colocalizes with AP-3, clathrin, Rab5 and Rab7b.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Lysosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Increased platelet account and deficiency in platelet alpha-granules asociated with bleeding diathesis and decreases aggregate formation; reduction of mature ype-II multivesicular bodies (MVB II) in megakaryocytes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 617616Vacuolar protein sorting-associated protein 33BPRO_0000206306Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Post-translational modificationi

Phosphorylated on tyrosine residues.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP59016.
MaxQBiP59016.
PaxDbiP59016.
PRIDEiP59016.

PTM databases

iPTMnetiP59016.
PhosphoSiteiP59016.

Expressioni

Gene expression databases

BgeeiP59016.
CleanExiMM_VPS33B.
ExpressionAtlasiP59016. baseline and differential.
GenevisibleiP59016. MM.

Interactioni

Subunit structurei

Interacts with RAB11A and VIPAS39 (PubMed:20190753). Associates with adaptor protein complex 3 (AP-3), clathrin:AP-3 and clathrin:HGS complexes (PubMed:21411634).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Rab11aP624923EBI-2656383,EBI-770256

Protein-protein interaction databases

IntActiP59016. 1 interaction.
STRINGi10090.ENSMUSP00000032749.

Structurei

3D structure databases

ProteinModelPortaliP59016.
SMRiP59016. Positions 14-614.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the STXBP/unc-18/SEC1 family.Curated

Phylogenomic databases

eggNOGiKOG1302. Eukaryota.
COG5158. LUCA.
GeneTreeiENSGT00530000063488.
HOGENOMiHOG000166771.
HOVERGENiHBG106182.
InParanoidiP59016.
OMAiSCDEWAF.
OrthoDBiEOG722J85.
PhylomeDBiP59016.
TreeFamiTF315126.

Family and domain databases

Gene3Di3.40.50.1910. 2 hits.
InterProiIPR027482. Sec-1-like_dom2.
IPR001619. Sec1-like.
IPR027121. VPS33.
[Graphical view]
PANTHERiPTHR11679. PTHR11679. 2 hits.
PTHR11679:SF1. PTHR11679:SF1. 2 hits.
PfamiPF00995. Sec1. 1 hit.
[Graphical view]
SUPFAMiSSF56815. SSF56815. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P59016-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFPHRLDAP ELPDFSMLKR LARDQLIYLL EQLPGKKDLF IEADLMSPLD
60 70 80 90 100
RIANVSILKQ HEVDKLYKVE NKPALSANEQ LCFLVRPRIK NMRYIASLVN
110 120 130 140 150
ADKLAGRIRK YKVILSPQKF YACEMVLEEE GVYGDVSCDE WAFSLLPLDV
160 170 180 190 200
DLLSMELPEF FRDYFLEGDQ RWINTVAQAL HLLSTLYGPF PNCYGIGRCA
210 220 230 240 250
KMSYDLWRKL EEEEDSETKG RKPEIGHIFL LDRDVDFVTA LCSQVVYEGL
260 270 280 290 300
VDDTFRIKCG SVDFGPEVTS SDKSLKVLLN AEDKVFSEIR NEHFSNVFGF
310 320 330 340 350
LSQKARNLQA QYDRRRGMDI KQMKNFVSQE LKGLKQEHRL LSLHIGACES
360 370 380 390 400
IMKKKTKQDF QELIKTEHAL LEGFNIREST SYIEEHIDRQ VSPIESLRLM
410 420 430 440 450
CLLSITENGL IPKDYRSLKT QYLQSYGPEH LLTFSNLRRA GLLTEQAPGD
460 470 480 490 500
TLTAVESKVS KLVTDKAAGK ITDAFSSLAK RSNFRAISKK LNLIPRVDGE
510 520 530 540 550
YDLKVPRDMA YVFSGAYVPL SCRIIEQVLD RRSWQGLDEV VRLLNCSEFA
560 570 580 590 600
FTDTAKEDKA SSESLRLILV VFLGGCTFSE ISALRFLGRE KGYRFIFLTT
610
AVTNSARLME AMSEVKS
Length:617
Mass (Da):70,526
Last modified:September 19, 2002 - v1
Checksum:iB6AA803F2EAD6397
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti275 – 2773LKV → ARG in AAH28783 (PubMed:15489334).Curated
Sequence conflicti315 – 3151R → W in AAH28783 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC028783 mRNA. Translation: AAH28783.1.
BC034170 mRNA. Translation: AAH34170.1.
CCDSiCCDS39997.1.
RefSeqiNP_835171.2. NM_178070.4.
UniGeneiMm.322870.

Genome annotation databases

EnsembliENSMUST00000032749; ENSMUSP00000032749; ENSMUSG00000030534.
GeneIDi233405.
KEGGimmu:233405.
UCSCiuc009iab.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC028783 mRNA. Translation: AAH28783.1.
BC034170 mRNA. Translation: AAH34170.1.
CCDSiCCDS39997.1.
RefSeqiNP_835171.2. NM_178070.4.
UniGeneiMm.322870.

3D structure databases

ProteinModelPortaliP59016.
SMRiP59016. Positions 14-614.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP59016. 1 interaction.
STRINGi10090.ENSMUSP00000032749.

PTM databases

iPTMnetiP59016.
PhosphoSiteiP59016.

Proteomic databases

EPDiP59016.
MaxQBiP59016.
PaxDbiP59016.
PRIDEiP59016.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032749; ENSMUSP00000032749; ENSMUSG00000030534.
GeneIDi233405.
KEGGimmu:233405.
UCSCiuc009iab.1. mouse.

Organism-specific databases

CTDi26276.
MGIiMGI:2446237. Vps33b.

Phylogenomic databases

eggNOGiKOG1302. Eukaryota.
COG5158. LUCA.
GeneTreeiENSGT00530000063488.
HOGENOMiHOG000166771.
HOVERGENiHBG106182.
InParanoidiP59016.
OMAiSCDEWAF.
OrthoDBiEOG722J85.
PhylomeDBiP59016.
TreeFamiTF315126.

Miscellaneous databases

PROiP59016.
SOURCEiSearch...

Gene expression databases

BgeeiP59016.
CleanExiMM_VPS33B.
ExpressionAtlasiP59016. baseline and differential.
GenevisibleiP59016. MM.

Family and domain databases

Gene3Di3.40.50.1910. 2 hits.
InterProiIPR027482. Sec-1-like_dom2.
IPR001619. Sec1-like.
IPR027121. VPS33.
[Graphical view]
PANTHERiPTHR11679. PTHR11679. 2 hits.
PTHR11679:SF1. PTHR11679:SF1. 2 hits.
PfamiPF00995. Sec1. 1 hit.
[Graphical view]
SUPFAMiSSF56815. SSF56815. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney and Mammary gland.
  2. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Kidney, Pancreas, Spleen and Testis.
  3. Cited for: FUNCTION, INTERACTION WITH RAB11A, KNOCKDOWN.
  4. "Clathrin-dependent mechanisms modulate the subcellular distribution of class C Vps/HOPS tether subunits in polarized and nonpolarized cells."
    Zlatic S.A., Tornieri K., L'Hernault S.W., Faundez V.
    Mol. Biol. Cell 22:1699-1715(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  5. "VPS33B regulates protein sorting into and maturation of alpha-granule progenitor organelles in mouse megakaryocytes."
    Bem D., Smith H., Banushi B., Burden J.J., White I.J., Hanley J., Jeremiah N., Rieux-Laucat F., Bettels R., Ariceta G., Mumford A.D., Thomas S.G., Watson S.P., Gissen P.
    Blood 126:133-143(2015) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiVP33B_MOUSE
AccessioniPrimary (citable) accession number: P59016
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: September 19, 2002
Last modified: June 8, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Vps33b-deficient inner medullary collecting duct cells display abnormal expression of membrane proteins such as Ceacam5, structural and functional tight junction defects and reduced E-cadherin expression.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.