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Protein

Vacuolar protein sorting-associated protein 33B

Gene

Vps33b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. Mediates phagolysosomal fusion in macrophages. Proposed to be involved in endosomal maturation implicating in part VIPAS39 (By similarity). In epithelial cells, the VPS33B:VIPAS39 complex may play a role in the apical RAB11A-dependentrecycling pathway and in the maintenance of the apical-basolateral polarity (PubMed:20190753). Seems to be involved in the sorting of specific cargos from the trans-Golgi network to alpha-granule-destined multivesicular bodies (MVBs) promoting MVBs maturation in megakaryocytes (PubMed:25947942).By similarity2 Publications

Miscellaneous

Vps33b-deficient inner medullary collecting duct cells display abnormal expression of membrane proteins such as Ceacam5, structural and functional tight junction defects and reduced E-cadherin expression.

GO - Biological processi

  • collagen fibril organization Source: MGI
  • collagen metabolic process Source: MGI
  • endosome organization Source: MGI
  • intracellular protein transport Source: MGI
  • lysosome localization Source: MGI
  • megakaryocyte development Source: UniProtKB
  • melanosome localization Source: MGI
  • membrane fusion Source: MGI
  • peptidyl-lysine hydroxylation Source: MGI
  • platelet alpha granule organization Source: UniProtKB
  • protein transport Source: MGI
  • regulation of platelet aggregation Source: UniProtKB
  • vesicle docking involved in exocytosis Source: InterPro
  • vesicle-mediated transport Source: MGI

Keywordsi

Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 33B
Gene namesi
Name:Vps33b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:2446237. Vps33b.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Lysosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Increased platelet account and deficiency in platelet alpha-granules associated with bleeding diathesis and decreases aggregate formation; reduction of mature ype-II multivesicular bodies (MVB II) in megakaryocytes.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002063062 – 617Vacuolar protein sorting-associated protein 33BAdd BLAST616

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosine residues.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP59016.
MaxQBiP59016.
PaxDbiP59016.
PeptideAtlasiP59016.
PRIDEiP59016.

PTM databases

iPTMnetiP59016.
PhosphoSitePlusiP59016.

Expressioni

Gene expression databases

BgeeiENSMUSG00000030534.
CleanExiMM_VPS33B.
ExpressionAtlasiP59016. baseline and differential.
GenevisibleiP59016. MM.

Interactioni

Subunit structurei

Interacts with RAB11A and VIPAS39 (PubMed:20190753). Associates with adaptor protein complex 3 (AP-3), clathrin:AP-3 and clathrin:HGS complexes (PubMed:21411634).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Rab11aP624923EBI-2656383,EBI-770256

Protein-protein interaction databases

IntActiP59016. 1 interactor.
STRINGi10090.ENSMUSP00000032749.

Structurei

3D structure databases

ProteinModelPortaliP59016.
SMRiP59016.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the STXBP/unc-18/SEC1 family.Curated

Phylogenomic databases

eggNOGiKOG1302. Eukaryota.
COG5158. LUCA.
GeneTreeiENSGT00530000063488.
HOGENOMiHOG000166771.
HOVERGENiHBG106182.
InParanoidiP59016.
OMAiSCDEWAF.
OrthoDBiEOG091G0AK0.
PhylomeDBiP59016.
TreeFamiTF315126.

Family and domain databases

InterProiView protein in InterPro
IPR001619. Sec1-like.
IPR036045. Sec1-like_sf.
IPR027121. VPS33.
PANTHERiPTHR11679. PTHR11679. 1 hit.
PTHR11679:SF1. PTHR11679:SF1. 1 hit.
PfamiView protein in Pfam
PF00995. Sec1. 1 hit.
SUPFAMiSSF56815. SSF56815. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P59016-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFPHRLDAP ELPDFSMLKR LARDQLIYLL EQLPGKKDLF IEADLMSPLD
60 70 80 90 100
RIANVSILKQ HEVDKLYKVE NKPALSANEQ LCFLVRPRIK NMRYIASLVN
110 120 130 140 150
ADKLAGRIRK YKVILSPQKF YACEMVLEEE GVYGDVSCDE WAFSLLPLDV
160 170 180 190 200
DLLSMELPEF FRDYFLEGDQ RWINTVAQAL HLLSTLYGPF PNCYGIGRCA
210 220 230 240 250
KMSYDLWRKL EEEEDSETKG RKPEIGHIFL LDRDVDFVTA LCSQVVYEGL
260 270 280 290 300
VDDTFRIKCG SVDFGPEVTS SDKSLKVLLN AEDKVFSEIR NEHFSNVFGF
310 320 330 340 350
LSQKARNLQA QYDRRRGMDI KQMKNFVSQE LKGLKQEHRL LSLHIGACES
360 370 380 390 400
IMKKKTKQDF QELIKTEHAL LEGFNIREST SYIEEHIDRQ VSPIESLRLM
410 420 430 440 450
CLLSITENGL IPKDYRSLKT QYLQSYGPEH LLTFSNLRRA GLLTEQAPGD
460 470 480 490 500
TLTAVESKVS KLVTDKAAGK ITDAFSSLAK RSNFRAISKK LNLIPRVDGE
510 520 530 540 550
YDLKVPRDMA YVFSGAYVPL SCRIIEQVLD RRSWQGLDEV VRLLNCSEFA
560 570 580 590 600
FTDTAKEDKA SSESLRLILV VFLGGCTFSE ISALRFLGRE KGYRFIFLTT
610
AVTNSARLME AMSEVKS
Length:617
Mass (Da):70,526
Last modified:September 19, 2002 - v1
Checksum:iB6AA803F2EAD6397
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti275 – 277LKV → ARG in AAH28783 (PubMed:15489334).Curated3
Sequence conflicti315R → W in AAH28783 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC028783 mRNA. Translation: AAH28783.1.
BC034170 mRNA. Translation: AAH34170.1.
CCDSiCCDS39997.1.
RefSeqiNP_835171.2. NM_178070.4.
UniGeneiMm.322870.

Genome annotation databases

EnsembliENSMUST00000032749; ENSMUSP00000032749; ENSMUSG00000030534.
GeneIDi233405.
KEGGimmu:233405.
UCSCiuc009iab.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiVP33B_MOUSE
AccessioniPrimary (citable) accession number: P59016
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: September 19, 2002
Last modified: October 25, 2017
This is version 119 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families