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Protein

Vacuolar protein sorting-associated protein 18 homolog

Gene

vps18

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport pathways. Believed to act as a core component of the putative HOPS endosomal tethering complex which is proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion (By similarity). May be involved in vesicle trafficking to the hepatocyte apical membrane and play a role in development of the intra-hepatic biliary tree. May target endosomes to the pigment granule in melanocytes. Essential for early embryonic development.By similarity2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri853 – 94896RING-typeAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • apical protein localization Source: ZFIN
  • bile acid and bile salt transport Source: ZFIN
  • endosome organization Source: GO_Central
  • endosome to lysosome transport Source: ZFIN
  • endosome to pigment granule transport Source: ZFIN
  • eye pigmentation Source: ZFIN
  • intracellular protein transport Source: InterPro
  • lysosome organization Source: GO_Central
  • notochord cell vacuolation Source: ZFIN
  • optokinetic behavior Source: ZFIN
  • pigment granule maturation Source: ZFIN
  • regulation of SNARE complex assembly Source: GO_Central
  • vesicle docking involved in exocytosis Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein sorting-associated protein 18 homolog
Gene namesi
Name:vps18
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 20

Organism-specific databases

ZFINiZDB-GENE-020419-33. vps18.

Subcellular locationi

  • Late endosome membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
  • Lysosome membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity

  • Note: Cytoplasmic, peripheral membrane protein associated with early endosomes and late endosomes/lysosomes.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endosome, Lysosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Mutants display hepatomegaly associated with large, vesicle-filled hepatocytes. They also have defects in pigmentation with reduced numbers of melanophores and no iridophores.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 974974Vacuolar protein sorting-associated protein 18 homologPRO_0000055904Add
BLAST

Proteomic databases

PaxDbiP59015.

Expressioni

Gene expression databases

BgeeiENSDARG00000070433.
ExpressionAtlasiP59015. differential.

Interactioni

Subunit structurei

Component of the homotypic fusion and vacuole protein sorting (HOPS) complex (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi7955.ENSDARP00000094129.

Structurei

3D structure databases

ProteinModelPortaliP59015.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati618 – 772155CHCRAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili461 – 48121Sequence analysisAdd
BLAST
Coiled coili799 – 85254Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the VPS18 family.Curated
Contains 1 CHCR (clathrin heavy-chain) repeat.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri853 – 94896RING-typeAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG2034. Eukaryota.
ENOG410XRSD. LUCA.
GeneTreeiENSGT00390000015233.
HOGENOMiHOG000172497.
HOVERGENiHBG057876.
InParanoidiP59015.
KOiK20181.
OMAiFAMRTCE.
OrthoDBiEOG091G01XI.
PhylomeDBiP59015.
TreeFamiTF105704.

Family and domain databases

InterProiIPR000547. Clathrin_H-chain/VPS_repeat.
IPR007810. Pep3_Vps18.
[Graphical view]
PfamiPF00637. Clathrin. 1 hit.
PF05131. Pep3_Vps18. 1 hit.
[Graphical view]
PROSITEiPS50236. CHCR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P59015-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASILDQYED SQNIRQHSRM STANIGITHS GFVNVRLEEE KPIFTKQRID
60 70 80 90 100
FSPPEKINQF SVCNNQLCMS LGKDTLLRID LGKPDQPNQI ELGRKDDSKV
110 120 130 140 150
HRLFLDPTGS HLVICLTTNE CVYLNRNTQK VRGLSRWRGH LIESIGWNKL
160 170 180 190 200
IGSETNTGPI LVGTSQGIIF EAEISASEGS LFNTNPDQYF RQVHYLEEDG
210 220 230 240 250
KPAPVCCLEV ERGLETKYFI IATTRKRLFQ FVGKLAEGSE QQGFSSIFAQ
260 270 280 290 300
NQDLLPSFQE FPVNMGYSEI TFYTSKLRSR PKTFAWMMGN GVLYGQLDYV
310 320 330 340 350
RPDSLLSDVQ VWEYTQDIDL NFVKPISIVL TQFHFLLLLP DRVRGICTLN
360 370 380 390 400
GQVVHEDVFP EKFGTLQKMI KDPITGLVWI YTEKAVFRYH IQKEARDVWQ
410 420 430 440 450
MYMNMNKFDL AKEYCKDRPE CLDMVLAKEA EHCFQNKRYL ESAKCYALTQ
460 470 480 490 500
NYFEEIALKF IEAKQEEALK EFLIKKLVNL KPSEKTQITL LVTWLTELYL
510 520 530 540 550
NRLGQLEADE GKQHLFLETR EEFRTFLKSP KHKDCFYNNR STIYDLLASH
560 570 580 590 600
GDVDNMVYFS VIMQDYERVI SHYCQHDDYS AALDVLSKHC DDKLFYKFSP
610 620 630 640 650
VLMQHIPKKV VDAWIQMGNR LDPKNLIPAL VNYSQMGSMQ QINETIRYME
660 670 680 690 700
FCVYELDVKE EAIHNYLLSL YAKHKPDALL WYLEQAGTHV SDIHYDLKYA
710 720 730 740 750
LRLCSEHGYL QACVLVYKIM ELYEEAVDLA LKVDVDLAKS CADLPEDDEE
760 770 780 790 800
LRKKLWLKIA RHVVQEEKDV KKAMNCLSSC NLLKIEDILP FFPDFVTIDH
810 820 830 840 850
FKEAICSSLE EYNKHIEELK QEMEEATESA KRIREDIQEM RNKYGVVESQ
860 870 880 890 900
EKCATCDFPL LNRPFYLFLC GHMFHYDCLL QEVIPHLSVY KQNKLDELQK
910 920 930 940 950
KLAATTQTTK ARHKPREEDT VSLGKGQGSR EQIKSDIDDI IACECVYCGE
960 970
LMIKSIDKPF IDPQKFDQEM SSWL
Length:974
Mass (Da):113,267
Last modified:May 1, 2007 - v2
Checksum:i05B5AE0D853BEC74
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti258 – 2603FQE → SRN in AAM34648 (PubMed:12006978).Curated
Sequence conflicti745 – 7451P → S in AAM34648 (PubMed:12006978).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL929049 Genomic DNA. Translation: CAI20654.1.
BX897725 Genomic DNA. Translation: CAI11898.1.
AF506204 mRNA. Translation: AAM34648.1.
BC086828 mRNA. Translation: AAH86828.1.
RefSeqiNP_775352.2. NM_173245.2.
UniGeneiDr.8966.

Genome annotation databases

EnsembliENSDART00000103352; ENSDARP00000094129; ENSDARG00000070433.
GeneIDi100005887.
KEGGidre:100005887.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL929049 Genomic DNA. Translation: CAI20654.1.
BX897725 Genomic DNA. Translation: CAI11898.1.
AF506204 mRNA. Translation: AAM34648.1.
BC086828 mRNA. Translation: AAH86828.1.
RefSeqiNP_775352.2. NM_173245.2.
UniGeneiDr.8966.

3D structure databases

ProteinModelPortaliP59015.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000094129.

Proteomic databases

PaxDbiP59015.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000103352; ENSDARP00000094129; ENSDARG00000070433.
GeneIDi100005887.
KEGGidre:100005887.

Organism-specific databases

CTDi57617.
ZFINiZDB-GENE-020419-33. vps18.

Phylogenomic databases

eggNOGiKOG2034. Eukaryota.
ENOG410XRSD. LUCA.
GeneTreeiENSGT00390000015233.
HOGENOMiHOG000172497.
HOVERGENiHBG057876.
InParanoidiP59015.
KOiK20181.
OMAiFAMRTCE.
OrthoDBiEOG091G01XI.
PhylomeDBiP59015.
TreeFamiTF105704.

Miscellaneous databases

PROiP59015.

Gene expression databases

BgeeiENSDARG00000070433.
ExpressionAtlasiP59015. differential.

Family and domain databases

InterProiIPR000547. Clathrin_H-chain/VPS_repeat.
IPR007810. Pep3_Vps18.
[Graphical view]
PfamiPF00637. Clathrin. 1 hit.
PF05131. Pep3_Vps18. 1 hit.
[Graphical view]
PROSITEiPS50236. CHCR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVPS18_DANRE
AccessioniPrimary (citable) accession number: P59015
Secondary accession number(s): Q5SPB8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: May 1, 2007
Last modified: September 7, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.