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Protein

dITP/XTP pyrophosphatase

Gene

MW1034

Organism
Staphylococcus aureus (strain MW2)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.UniRule annotation

Catalytic activityi

XTP + H2O = XMP + diphosphate.UniRule annotation
dITP + H2O = dIMP + diphosphate.UniRule annotation
ITP + H2O = IMP + diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi39MagnesiumUniRule annotation1
Active sitei68Proton acceptorUniRule annotation1
Metal bindingi68MagnesiumUniRule annotation1
Binding sitei69Substrate; via amide nitrogenUniRule annotation1
Binding sitei172SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
dITP/XTP pyrophosphataseUniRule annotation (EC:3.6.1.66UniRule annotation)
Alternative name(s):
Non-canonical purine NTP pyrophosphataseUniRule annotation
Non-standard purine NTP pyrophosphataseUniRule annotation
Nucleoside-triphosphate diphosphataseUniRule annotation
Nucleoside-triphosphate pyrophosphataseUniRule annotation
Short name:
NTPaseUniRule annotation
Gene namesi
Ordered Locus Names:MW1034
OrganismiStaphylococcus aureus (strain MW2)
Taxonomic identifieri196620 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000418 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001782321 – 195dITP/XTP pyrophosphataseAdd BLAST195

Proteomic databases

PRIDEiP58995

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP58995
SMRiP58995
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni8 – 13Substrate bindingUniRule annotation6
Regioni149 – 152Substrate bindingUniRule annotation4
Regioni177 – 178Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the HAM1 NTPase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000293319
KOiK02428
OMAiYDPIFQP

Family and domain databases

CDDicd00515 HAM1, 1 hit
Gene3Di3.90.950.10, 1 hit
HAMAPiMF_01405 Non_canon_purine_NTPase, 1 hit
InterProiView protein in InterPro
IPR020922 dITP/XTP_pyrophosphatase
IPR002637 Ham1p-like
IPR029001 ITPase-like_fam
PANTHERiPTHR11067 PTHR11067, 1 hit
PfamiView protein in Pfam
PF01725 Ham1p_like, 1 hit
SUPFAMiSSF52972 SSF52972, 1 hit
TIGRFAMsiTIGR00042 TIGR00042, 1 hit

Sequencei

Sequence statusi: Complete.

P58995-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEIVIASNN QGKINDFKVI FPDYHVIGIS ELIPDFDVEE TGSTFEENAI
60 70 80 90 100
LKSEAAAKAL NKTVIADDSG LEVFALNGEP GIYSARYAGE NKSDEANIEK
110 120 130 140 150
LLNKLGNTTD RRAQFVCVIS MSGPDMETKV FKGTVSGEIA DGKYGENGFG
160 170 180 190
YDPIFYVPKL DKTMAQLSKE QKGQISHRRN AINLLQAFLE GDKNV
Length:195
Mass (Da):21,403
Last modified:August 30, 2002 - v1
Checksum:iB5810D3DC6ECE80D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000033 Genomic DNA Translation: BAB94899.1
RefSeqiWP_000659317.1, NC_003923.1

Genome annotation databases

EnsemblBacteriaiBAB94899; BAB94899; BAB94899
KEGGisam:MW1034

Similar proteinsi

Entry informationi

Entry nameiIXTPA_STAAW
AccessioniPrimary (citable) accession number: P58995
Secondary accession number(s): Q8NX54
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: August 30, 2002
Last modified: March 28, 2018
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health