P58989 (CRA1_HOMGA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Crustacyanin-A1 subunit
Organism	Homarus gammarus (European lobster) (Homarus vulgaris)
Taxonomic identifier	6707 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Crustacea › Malacostraca › Eumalacostraca › Eucarida › Decapoda › Pleocyemata › Astacidea › Nephropoidea › Nephropidae › Homarus

Protein attributes

Sequence length	181 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds the carotenoid astaxanthin (AXT) which provides the blue coloration to the carapace of the lobster.
Subunit structure	Oligomer; Can form dimers (beta-crustacyanin); or complexes of 16 subunits (alpha-crustacyanin). There are five types of subunits: A1, A2, A3, C1 and C2.
Subcellular location	Secreted › extracellular space.
Tissue specificity	Found in the carapace.
Sequence similarities	Belongs to the calycin superfamily. Lipocalin family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Secreted
Ligand	Chromophore Pigment
PTM	Disulfide bond
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	pigment binding Inferred from electronic annotation. Source: UniProtKB-KW small molecule binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 181

181

Crustacyanin-A1 subunit

PRO_0000201009

Amino acid modifications

Disulfide bond

12 ↔ 121

Disulfide bond

51 ↔ 173

Disulfide bond

117 ↔ 150

Secondary structure

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181

Helix Strand Turn

Details...

Beta strand

7 – 11

5

Helix

17 – 24

8

Helix

25 – 27

3

Helix

28 – 31

4

Beta strand

33 – 41

9

Beta strand

48 – 58

11

Beta strand

63 – 71

9

Beta strand

74 – 85

12

Beta strand

91 – 97

7

Beta strand

98 – 100

3

Beta strand

102 – 110

9

Beta strand

112 – 123

12

Beta strand

127 – 141

15

Helix

144 – 156

13

Helix

161 – 163

3

Beta strand

164 – 166

3

Beta strand

170 – 173

4

Helix

175 – 178

4

Sequences

	Sequence		Length	Mass (Da)
	P58989 [UniParc]. Last modified August 13, 2002. Version 1. Checksum: 285EADC1A4F2E353	FASTA	181	20,652
	10 20 30 40 50 60 DKIPNFVVPG KCASVDRNKL WAEQTPNRNS YAGVWYQFAL TNNPYQLIEK CVRNEYSFDG 70 80 90 100 110 120 KQFVIKSTGI AYDGNLLKRN GKLYPNPFGE PHLSIDYENS FAAPLVILET DYSNYACLYS 130 140 150 160 170 180 CIDYNFGYHS DFSFIFSRSA NLADQYVKKC EAAFKNINVD TTRFVKTVQG SSCPYDTQKT V

References

[1]	"Structure of lobster apocrustacyanin A1 using softer X-rays." Cianci M., Rizkallah P.J., Olczak A., Raftery J., Chayen N.E., Zagalsky P.F., Helliwell J.R. Acta Crystallogr. D 57:1219-1229(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.15 ANGSTROMS) OF HOMODIMER.
[2]	"The molecular basis of the coloration mechanism in lobster shell: beta-crustacyanin at 3.2-A resolution." Cianci M., Rizkallah P.J., Olczak A., Raftery J., Chayen N.E., Zagalsky P.F., Helliwell J.R. Proc. Natl. Acad. Sci. U.S.A. 99:9795-9800(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS) OF HETERODIMER OF A1 AND A3 IN COMPLEX WITH ASTAXANTHIN.
+	Additional computationally mapped references.

Web resources

Protein Spotlight

Squeeze me - Issue 26 of September 2002

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GKA	X-ray	3.23	A	2-181	[»]
1S44	X-ray	1.60	A/B	2-181	[»]

ProteinModelPortal

P58989.

SMR

P58989. Positions 2-181.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-242140.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.40.128.20. 1 hit.

InterPro

IPR012674. Calycin.
IPR011038. Calycin-like.
IPR003057. Invtbrt_color.
IPR002345. Lipocalin.
IPR022271. Lipocalin_ApoD.
IPR000566. Lipocln_cytosolic_FA-bd_dom.
[Graphical view]

Pfam

PF00061. Lipocalin. 1 hit.
[Graphical view]

PIRSF

PIRSF036893. Lipocalin_ApoD. 1 hit.

PRINTS

PR01273. INVTBRTCOLOR.
PR00179. LIPOCALIN.

SUPFAM

SSF50814. Calycin. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P58989.

Entry information

Entry name

CRA1_HOMGA

Accession

Primary (citable) accession number: P58989

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 2002
Last sequence update:	August 13, 2002
Last modified:	April 3, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)