P58935 (GUN_XANAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Endoglucanase
EC=3.2.1.4

Alternative name(s):
Carboxymethylcellulase
Short name=CMCase
Cellulase
Endo-1,4-beta-glucanase

Gene names

Name:	bcsZ
Ordered Locus Names:	XAC3516

Organism

Xanthomonas axonopodis pv. citri (strain 306) [Complete proteome] [HAMAP]

Taxonomic identifier

190486 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas › Xanthomonas citri group › Xanthomonas campestris pv. citri › Xanthomonas citri pv. citri

Protein attributes

Sequence length	384 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Hydrolyzes carboxymethylcellulose By similarity.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Pathway	Glycan metabolism; bacterial cellulose biosynthesis.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 8 (cellulase D) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 384	359	Endoglucanase		PRO_0000007943
Sites
	Active site	63	1	Proton donor By similarity
	Active site	124	1	Nucleophile Potential

Sequences

	Sequence		Length	Mass (Da)
	P58935 [UniParc]. Last modified July 26, 2002. Version 1. Checksum: 4DD7FB6CF822D127	FASTA	384	41,510
	10 20 30 40 50 60 MTRRRLLHAG TLAGVAALLP AAALAAPSQC GPWPLWSAFV DKHIQRDGRV VDFLNPDQRS 70 80 90 100 110 120 TSEGQSYALF FALVNNDQVL FDKVLSWTRH NLCGGRPDLN LPAWLWGRDG SGAWRVLDAN 130 140 150 160 170 180 TASDGELWIA YALLEAGRLW SRPGFLKAGQ QMLQLIRTQE VATLPGLGPM LLPGRTGFVD 190 200 210 220 230 240 NGRWTLNPSY LPIQVLRRCA NADPKGPWAA IAANSARVLR DSAPVGFAPD WTVWDGKTFN 250 260 270 280 290 300 ADPKRGNVGS YDAIRVYLWA GMLDAGEPLR ARLLQDLSGP ADLLAAQQTP AEKIDTARGV 310 320 330 340 350 360 GTGALPVGFS AALLPYLSAL GKPALLKAQA QRVPAATQPA AAALPYFERT LALFGQGWLE 370 380 NRYRFAADGR LLPAWRTPAC AATT

References

[1]

"Comparison of the genomes of two Xanthomonas pathogens with differing host specificities." da Silva A.C.R., Ferro J.A., Reinach F.C., Farah C.S., Furlan L.R., Quaggio R.B., Monteiro-Vitorello C.B., Van Sluys M.A., Almeida N.F. Jr., Alves L.M.C., do Amaral A.M., Bertolini M.C., Camargo L.E.A., Camarotte G., Cannavan F., Cardozo J., Chambergo F., Ciapina L.P. , Cicarelli R.M.B., Coutinho L.L., Cursino-Santos J.R., El-Dorry H., Faria J.B., Ferreira A.J.S., Ferreira R.C.C., Ferro M.I.T., Formighieri E.F., Franco M.C., Greggio C.C., Gruber A., Katsuyama A.M., Kishi L.T., Leite R.P., Lemos E.G.M., Lemos M.V.F., Locali E.C., Machado M.A., Madeira A.M.B.N., Martinez-Rossi N.M., Martins E.C., Meidanis J., Menck C.F.M., Miyaki C.Y., Moon D.H., Moreira L.M., Novo M.T.M., Okura V.K., Oliveira M.C., Oliveira V.R., Pereira H.A., Rossi A., Sena J.A.D., Silva C., de Souza R.F., Spinola L.A.F., Takita M.A., Tamura R.E., Teixeira E.C., Tezza R.I.D., Trindade dos Santos M., Truffi D., Tsai S.M., White F.F., Setubal J.C., Kitajima J.P. Nature 417:459-463(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 306.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE008923 Genomic DNA. Translation: AAM38359.1.
RefSeq	NP_643823.2. NC_003919.1.
3D structure databases
ProteinModelPortal	P58935.
ModBase	Search...
Protein-protein interaction databases
STRING	190486.XAC3516.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM38359; AAM38359; XAC3516.
GeneID	1157587.
KEGG	xac:XAC3516.
PATRIC	24059174. VBIXanAxo33670_3638.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3405.
HOGENOM	HOG000259088.
KO	K01179.
OMA	IRVYLWV.
ProtClustDB	PRK11097.
Enzyme and pathway databases
UniPathway	UPA00694.
Family and domain databases
Gene3D	1.50.10.10. 1 hit.
InterPro	IPR008928. 6-hairpin_glycosidase-like. IPR012341. 6hp_glycosidase. IPR002037. Glyco_hydro_8. [Graphical view]
Pfam	PF01270. Glyco_hydro_8. 1 hit. [Graphical view]
PRINTS	PR00735. GLHYDRLASE8.
SUPFAM	SSF48208. Glyco_trans_6hp. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GUN_XANAC

Accession

Primary (citable) accession number: P58935

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	July 26, 2002
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program