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P58932 (BCSA_XANAC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Cellulose synthase catalytic subunit [UDP-forming]

EC=2.4.1.12
Gene names
Name:bcsA
Ordered Locus Names:XAC3518
OrganismXanthomonas axonopodis pv. citri (strain 306) [Complete proteome] [HAMAP]
Taxonomic identifier190486 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas

Protein attributes

Sequence length729 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalytic subunit of cellulose synthase. It polymerizes uridine 5'-diphosphate glucose to cellulose, which is produced as an extracellular component for mechanical and chemical protection By similarity.

Catalytic activity

UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1).

Cofactor

Magnesium By similarity.

Enzyme regulation

Activated by bis-(3'-5') cyclic diguanylic acid (c-di-GMP) By similarity.

Pathway

Glycan metabolism; bacterial cellulose biosynthesis.

Subcellular location

Cell inner membrane; Multi-pass membrane protein Potential.

Domain

There are two conserved domains in the globular part of the protein: the N-terminal domain (domain A) contains the conserved DXD motif and is possibly involved in catalysis and substrate binding. The C-terminal domain (domain B) contains the QXXRW motif and is present only in processive glycosyl transferases. It could be involved in the processivity function of the enzyme, possibly required for holding the growing glycan chain in the active site.

Sequence similarities

Belongs to the glycosyltransferase 2 family.

Contains 1 PilZ domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 729729Cellulose synthase catalytic subunit [UDP-forming]
PRO_0000059272

Regions

Transmembrane30 – 5021Helical; Potential
Transmembrane110 – 13021Helical; Potential
Transmembrane171 – 19121Helical; Potential
Transmembrane405 – 42521Helical; Potential
Transmembrane427 – 44721Helical; Potential
Transmembrane520 – 54021Helical; Potential
Transmembrane549 – 56921Helical; Potential
Transmembrane610 – 63021Helical; Potential
Domain575 – 67197PilZ
Region151 – 24494Catalytic subdomain A
Region321 – 38161Catalytic subdomain B

Sites

Active site1931 Potential
Active site3371 Potential
Binding site2401Substrate Potential
Binding site2421Substrate Potential

Sequences

Sequence LengthMass (Da)Tools
P58932 [UniParc].

Last modified July 26, 2002. Version 1.
Checksum: B9C08BB995E795B1

FASTA72980,915
        10         20         30         40         50         60 
MPLVVPHAAM PEGRLMTAAS RRSASPLPTL ATWALWLLGA LLLVFVVAVP MDVTQQLVFS 

        70         80         90        100        110        120 
GVLFAVALAV RNRGGRVVIL MMMGMSLAVS CRYIWWRMTQ TMGVGSAVDF ILGLGLLGAE 

       130        140        150        160        170        180 
LYAFVILVLG YFQVLWPLNR KPVPLPADQR LWPSVDVFIP TYNEPLSVVR TTVLAASVID 

       190        200        210        220        230        240 
WPAGKITIHL LDDGRRDEFR AFCAEVGINY VTRTNNAHAK AGNINAALKK CSGDYVAIFD 

       250        260        270        280        290        300 
CDHIPTRSFL QVAMGWFLHD TKLALVQMPH YFFSPDPFER NLDTHGKVPN EGELFYGLLQ 

       310        320        330        340        350        360 
DGNDQWNATF FCGSCAVIKR TALEEVGGVA VETVTEDAHT ALKLQRRGYR TAYLAVPQAA 

       370        380        390        400        410        420 
GLATESLSGH VAQRIRWARG MAQIARIDNP LLGRGLKLSQ RLCYLNAMLH FFYGVPRIIY 

       430        440        450        460        470        480 
LTAPLAYLFF GAHVIQASAL MILAYALPHI LQANLTNLRV QSRFRHLLWN EVYETTLAWY 

       490        500        510        520        530        540 
IFRPTLVALL NPKLGKFNVT PKGGLVARSY FDAQIAKPYL FLLLLNVVGM VAGVLRLIYV 

       550        560        570        580        590        600 
GGSGEQQTIW FNLAWTLYNM VLLGATIATA SETRQVRSAH RVPLDVPVTL YLPDGDVLPS 

       610        620        630        640        650        660 
RSVNFSTGGM AIMLAQPQPI EPGLPVQIGL SHRGVEQTLP AVVRQDRDGQ VSIQFTQMSM 

       670        680        690        700        710        720 
EQERWLVAST FARADIWLSQ WGQHDRDAFW RSMGQVLEAS ARGFGRLGGH IVDSARQGFR 


PRRAVDLES 

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE008923 Genomic DNA. Translation: AAM38361.1.
RefSeqNP_643825.2. NC_003919.1.

3D structure databases

ProteinModelPortalP58932.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING190486.XAC3518.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAM38361; AAM38361; XAC3518.
GeneID1157589.
KEGGxac:XAC3518.
PATRIC24059178. VBIXanAxo33670_3640.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1215.
HOGENOMHOG000259144.
KOK00694.
OMAQLTMGWF.
OrthoDBEOG66TG3C.

Enzyme and pathway databases

BioCycXAXO190486:GH55-3518-MONOMER.
UniPathwayUPA00694.

Family and domain databases

InterProIPR003919. Cell_synth_A.
IPR009875. PilZ_domain.
[Graphical view]
PfamPF07238. PilZ. 1 hit.
[Graphical view]
PRINTSPR01439. CELLSNTHASEA.
TIGRFAMsTIGR03030. CelA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBCSA_XANAC
AccessionPrimary (citable) accession number: P58932
Entry history
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: July 26, 2002
Last modified: February 19, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways