P58931 (BCSA_PSEFS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cellulose synthase catalytic subunit [UDP-forming]
EC=2.4.1.12

Gene names

Name:	bcsA
Synonyms:	wssB
Ordered Locus Names:	PFLU_0301

Organism

Pseudomonas fluorescens (strain SBW25) [Complete proteome] [HAMAP]

Taxonomic identifier

216595 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	739 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalytic subunit of cellulose synthase. It polymerizes uridine 5'-diphosphate glucose to cellulose, which is produced as an extracellular component responsible for the structural integrity and rigidity of self-supporting mats characteristic of the "wrinkly spreader" phenotype.
Catalytic activity	UDP-glucose + (1,4-beta-D-glucosyl)(n) = UDP + (1,4-beta-D-glucosyl)(n+1).
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by bis-(3'-5') cyclic diguanylic acid (c-di-GMP) By similarity.
Pathway	Glycan metabolism; bacterial cellulose biosynthesis.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential.
Domain	There are two conserved domains in the globular part of the protein: the N-terminal domain (domain A) contains the conserved DXD motif and is possibly involved in catalysis and substrate binding. The C-terminal domain (domain B) contains the QXXRW motif and is present only in processive glycosyl transferases. It could be involved in the processivity function of the enzyme, possibly required for holding the growing glycan chain in the active site.
Sequence similarities	Belongs to the glycosyltransferase 2 family. Contains 1 PilZ domain.

Ontologies

Keywords
Biological process	Cellulose biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	c-di-GMP
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	UDP-glucose metabolic process Inferred from electronic annotation. Source: InterPro cellulose biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cellulose synthase (UDP-forming) activity Inferred from electronic annotation. Source: EC cyclic-di-GMP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 739	739	Cellulose synthase catalytic subunit [UDP-forming]		PRO_0000059271
Regions
	Transmembrane	36 – 55	20	Helical; Potential
	Transmembrane	59 – 76	18	Helical; Potential
	Transmembrane	83 – 101	19	Helical; Potential
	Transmembrane	116 – 138	23	Helical; Potential
	Transmembrane	417 – 436	20	Helical; Potential
	Transmembrane	440 – 462	23	Helical; Potential
	Transmembrane	524 – 546	23	Helical; Potential
	Transmembrane	551 – 573	23	Helical; Potential
	Domain	580 – 677	98	PilZ
	Region	157 – 250	94	Catalytic subdomain A
	Region	327 – 387	61	Catalytic subdomain B
Sites
	Active site	199	1	Potential
	Active site	343	1	Potential
	Binding site	246	1	Substrate Potential
	Binding site	248	1	Substrate Potential
Experimental info
	Sequence conflict	415	1	L → Q in AAL71842. Ref.1
	Sequence conflict	644	1	F → K in AAL71842. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P58931 [UniParc]. Last modified July 7, 2009. Version 2. Checksum: BFA57F3987F3F982	FASTA	739	82,169
	10 20 30 40 50 60 MTDTTSSTPF VEGRAEQRLN GAIARFNRWP SAPRTVLVVA SCVLGAMLLL GIISAPLDLY 70 80 90 100 110 120 SQCLFAAVCF LAVLVLRKIP GRLAILALVV LSLVASLRYM FWRLTSTLGF ETWVDMFFGY 130 140 150 160 170 180 GLVAAEFYAL IVLIFGYVQT AWPLRRTPVW LKTEPEEWPT VDVFIPTYNE ALSIVKLTIF 190 200 210 220 230 240 AAQAMDWPKD KLRVHVLDDG RRDDFREFCR KVGVNYIRRD NNFHAKAGNL NEALKVTDGE 250 260 270 280 290 300 YIALFDADHV PTRSFLQVSL GWFLKDPKLA MLQTPHFFFS PDPFEKNLDT FRAVPNEGEL 310 320 330 340 350 360 FYGLVQDGND LWNATFFCGS CAVIRREPLL EIGGVAVETV TEDAHTALKL NRLGYNTAYL 370 380 390 400 410 420 AIPQAAGLAT ESLSRHINQR IRWARGMAQI FRTDNPLLGK GLKWGQRICY ANAMLHFFYG 430 440 450 460 470 480 LPRLVFLTAP LAYLIFGAEI FHASALMIVA YVLPHLVHSS LTNSRIQGRF RHSFWNEVYE 490 500 510 520 530 540 TVLAWYILPP VLVALVNPKA GGFNVTDKGG IIDKQFFDWK LARPYLVLLA VNLIGLGFGI 550 560 570 580 590 600 HQLIWGDAST AVTVAINLTW TLYNLIITSA AVAVASEARQ VRSEPRVSAK LPVSIICADG 610 620 630 640 650 660 RVLDGTTQDF SQNGFGLMLS DGHSITQGER VQLVLSRNGQ DSLFDARVVF SKGAQIGAQF 670 680 690 700 710 720 EALSLRQQSE LVRLTFSRAD TWAASWGAGQ PDTPLAALRE VGSIGIGGLF TLGRATLHEL 730 RLALSRTPTK PLDTLMDKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Adaptive divergence in experimental populations of Pseudomonas fluorescens. I. Genetic and phenotypic bases of wrinkly spreader fitness." Spiers A.J., Kahn S.G., Bohannon J., Travisano M., Rainey P.B. Genetics 161:33-46(2002) [PubMed: 12019221] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genomic and genetic analyses of diversity and plant interactions of Pseudomonas fluorescens." Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R., Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M., Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J., Harris S., Challis G.L., Yaxley A.M., Harris D. , Seeger K., Murphy L., Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K., Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M., Parkhill J., Levy S.B., Rainey P.B., Thomson N.R. Genome Biol. 10:R51.1-R51.16(2009) [PubMed: 19432983] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SBW25.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY074776 Genomic DNA. Translation: AAL71842.1. AM181176 Genomic DNA. Translation: CAY46578.1.
RefSeq	YP_002869982.1. NC_012660.1.
3D structure databases
ProteinModelPortal	P58931.
ModBase	Search...
Protein-protein interaction databases
STRING	P58931.
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7816405.
GenomeReviews	Gene locus PFLU_0301 in contig AM181176_GR.
KEGG	pfs:PFLU0301.
PATRIC	19894806. VBIPseFlu98510_0534.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CYANAML.
ProtClustDB	CLSK2485084.
Enzyme and pathway databases
BioCyc	PFLU216595:PFLU0301-MONOMER.
Family and domain databases
InterPro	IPR003919. Cell_synth_A. IPR005150. Cellulose_synth. IPR001173. Glyco_trans_2. IPR009875. PilZ_domain. [Graphical view]
KO	K00694.
Pfam	PF03552. Cellulose_synt. 1 hit. PF00535. Glycos_transf_2. 1 hit. PF07238. PilZ. 1 hit. [Graphical view]
PRINTS	PR01439. CELLSNTHASEA.
TIGRFAMs	TIGR03030. CelA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BCSA_PSEFS

Accession

Primary (citable) accession number: P58931
Secondary accession number(s): C3K5V4, Q8RSZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	July 7, 2009
Last modified:	January 25, 2012
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program