P58928 (CEX_CONCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Conotoxin CcTx Alternative name(s): Excitotoxin CcTx
Organism	Conus consors (Singed cone)
Taxonomic identifier	101297 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Mollusca › Gastropoda › Caenogastropoda › Hypsogastropoda › Neogastropoda › Conoidea › Conidae › Conus

Protein attributes

Sequence length	30 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May specifically activate neuronal voltage-gated sodium channels (Nav) at the resting membrane potential. Causes a marked contraction and extension of the caudal and dorsal fins in fish and noticeable spontaneous contractions of isolated frog neuromuscular preparations.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom duct.
Domain	The cysteine framework is IV (CC-C-C-C-C).
Post-translational modification	Contains 3 disulfide bonds. They are not added, since framework IV presents two different connectivities (I-V, II-III, IV-VI and I-III, II-V, IV-VI).
Miscellaneous	Found in both injectable (milked) (IV) and dissected venom (DV) (Ref.2).
Mass spectrometry	Molecular mass is 4156 Da from positions 1 - 30. Determined by ESI. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Ion channel impairing toxin Neurotoxin Presynaptic neurotoxin Sodium channel inhibitor Toxin
PTM	Disulfide bond Glycoprotein Hydroxylation
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Cellular_component	other organism presynaptic membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	sodium channel inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Peptide

1 – 30

30

Conotoxin CcTx

PRO_0000044514

Amino acid modifications

Modified residue

2

1

4-hydroxyproline

Modified residue

17

1

4-hydroxyproline

Modified residue

22

1

4-hydroxyproline

Glycosylation

7

1

O-linked (HexNAc...) Ref.2

Secondary structure

1

.

.

.

30

Helix Strand Turn

Details...

Helix

23 – 26

4

Sequences

	Sequence		Length	Mass (Da)
	P58928 [UniParc]. Last modified March 23, 2010. Version 2. Checksum: AD8E9CC5ABB0E065	FASTA	30	3,184
	10 20 30 APWLVPSQIT TCCGYNPGTM CPSCMCTNTC

References

[1]	"A new conotoxin isolated from Conus consors venom acting selectively on axons and motor nerve terminals through a Na+-dependent mechanism." Le Gall F., Favreau P., Benoit E., Mattei C., Bouet F., Menou J.-L., Menez A., Letourneux Y., Molgo J. Eur. J. Neurosci. 11:3134-3142(1999) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, MASS SPECTROMETRY. Tissue: Venom.
[2]	"Comparative proteomic study of the venom of the piscivorous cone snail Conus consors." Biass D., Dutertre S., Gerbault A., Menou J.L., Offord R., Favreau P., Stocklin R. J. Proteomics 72:210-218(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO 7, GLYCOSYLATION AT SER-7.
[3]	"Large-scale discovery of conopeptides and conoproteins in the injectable venom of a fish-hunting cone snail using a combined proteomic and transcriptomic approach." Violette A., Biass D., Dutertre S., Koua D., Piquemal D., Pierrat F., Stocklin R., Favreau P. J. Proteomics 75:5215-5225(2012) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY OF GLYCOSYLATED AND NON-GLYCOSYLATED FORMS. Tissue: Venom.

Cross-references

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4B1Q	NMR	-	P	1-30	[»]

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Organism-specific databases

ConoServer

1510. CcTx.

Family and domain databases

ProtoNet

Search...

Entry information

Entry name

CEX_CONCN

Accession

Primary (citable) accession number: P58928

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 26, 2002
Last sequence update:	March 23, 2010
Last modified:	May 1, 2013
This is version 41 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Animal Toxin Annotation Program