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P58913

- CO16A_CONPU

UniProt

P58913 - CO16A_CONPU

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Protein

Delta-conotoxin PVIA

Gene
N/A
Organism
Conus purpurascens (Purple cone)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Delta-conotoxins bind to site 6 of voltage-gated sodium channels (Nav) and inhibit the inactivation process. In mice, injection of this toxin causes hyperactivity, rapid running, limb extension, and death. In fish, the peptide elicites spurts of rapid swimming, with twisted motions, quivering fins and the lockjaw extended mouth syndrome. Rigid paralysis and death are observed at higher doses. In mollusks, this peptide is inactive. Injection of this peptide together with the kappa-conotoxin PVIIA causes the sudden tetanus of prey (STOP) syndrome, which is a single, lethal "fin-pop" in envenomed fish.

GO - Molecular functioni

  1. sodium channel inhibitor activity Source: InterPro

GO - Biological processi

  1. pathogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-conotoxin PVIA
Alternative name(s):
Lockjaw peptide
OrganismiConus purpurascens (Purple cone)
Taxonomic identifieri41690 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaHypsogastropodaNeogastropodaConoideaConidaeConus

Organism-specific databases

ConoServeri1563. PVIA precursor.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi59 – 591T → A: No loss of activity. 1 Publication
Mutagenesisi60 – 601F → A: Loss of biological activity. However, coinjection of this mutant protects against the wild-type conotoxin, suggesting that the mutant binds competitively to the wild-type conotoxin ligand site. Such a protective effect does not occur for all physiological targets. 1 Publication
Mutagenesisi63 – 631I → A: Loss of activity. 1 Publication
Mutagenesisi64 – 641K → A: No loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 Reviewed predictionAdd
BLAST
Propeptidei23 – 4927 By similarityPRO_0000034898Add
BLAST
Peptidei52 – 8029Delta-conotoxin PVIA2 PublicationsPRO_0000034899Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi54 ↔ 69 By similarity
Modified residuei57 – 5714-hydroxyproline1 Publication
Disulfide bondi61 ↔ 73 By similarity
Modified residuei65 – 6514-hydroxyproline1 Publication
Disulfide bondi68 ↔ 78 By similarity
Modified residuei80 – 801Glycine amide; in form delta-conotoxin PVIA

Post-translational modificationi

The difference between delta-conotoxin PVIA and [deamido]-delta-conotoxin PVIA lies in the state of amidation of Gly-80.

Keywords - PTMi

Amidation, Cleavage on pair of basic residues, Disulfide bond, Hydroxylation

Expressioni

Tissue specificityi

Expressed by the venom duct.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin By similarity.
The cysteine framework is VI/VII (C-C-CC-C-C).

Sequence similaritiesi

Belongs to the conotoxin O1 superfamily.

Keywords - Domaini

Knottin, Signal

Family and domain databases

InterProiIPR004214. Conotoxin.
IPR012322. Conotoxin_d-typ_CS.
[Graphical view]
PfamiPF02950. Conotoxin. 1 hit.
[Graphical view]
PROSITEiPS60005. DELTA_CONOTOXIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P58913-1 [UniParc]FASTAAdd to Basket

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MKLTCVMIVA VLFLTAWTFV TADDSKNGLE NHFWKARDEM KNREASKLDK   50
KEACYAPGTF CGIKPGLCCS EFCLPGVCFG G 81
Length:81
Mass (Da):8,945
Last modified:July 26, 2002 - v1
Checksum:iACCFE9E1C65ABFEC
GO

Mass spectrometryi

Molecular mass is 2997.3 Da from positions 52 - 80. 1 Publication

Sequence databases

PIRiA58651.

Cross-referencesi

Sequence databases

PIRi A58651.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

ConoServeri 1563. PVIA precursor.

Family and domain databases

InterProi IPR004214. Conotoxin.
IPR012322. Conotoxin_d-typ_CS.
[Graphical view ]
Pfami PF02950. Conotoxin. 1 hit.
[Graphical view ]
PROSITEi PS60005. DELTA_CONOTOXIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Purification, characterization, synthesis, and cloning of the lockjaw peptide from Conus purpurascens venom."
    Shon K.-J., Grilley M.M., Marsh M., Yoshikami D., Hall A.R., Kurz B., Gray W.R., Imperial J.S., Hillyard D.R., Olivera B.M.
    Biochemistry 34:4913-4918(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 52-80, SYNTHESIS OF 52-80, HYDROXYLATION AT PRO-57 AND PRO-65, MASS SPECTROMETRY.
    Tissue: Venom and Venom duct.
  2. "Strategy for rapid immobilization of prey by a fish-hunting marine snail."
    Terlau H., Shon K.-J., Grilley M.M., Stocker M., Stuehmer W., Olivera B.M.
    Nature 381:148-151(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 52-80, SYNTHESIS OF 52-80.
  3. Cited for: SYNTHESIS OF 52-80, MUTAGENESIS OF THR-59; PHE-60; ILE-63 AND LYS-64.

Entry informationi

Entry nameiCO16A_CONPU
AccessioniPrimary (citable) accession number: P58913
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: July 26, 2002
Last modified: October 16, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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