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Protein

Kurtoxin

Gene
N/A
Organism
Parabuthus transvaalicus (South African fattail scorpion)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Alpha toxins bind voltage-independently at site-3 of sodium channels and inhibit the inactivation of the activated channels, thereby blocking neuronal transmission. This toxin acts on Nav1.2/SCN2A. Also binds to Cav3.1/CACNA1G and Cav3.2/CACNA1H T-type calcium channels with high affinity and inhibits the channels by modifying voltage-dependent gating. Another study (PubMed:11896142) shows that it also targets neuronal high-threshold calcium channels, including P-type, N-type, and L-type calcium channels (Cav), and others that still are unidentified pharmacologically.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Calcium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated calcium channel impairing toxin, Voltage-gated sodium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Kurtoxin
Short name:
Ktx
OrganismiParabuthus transvaalicus (South African fattail scorpion)
Taxonomic identifieri170972 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesButhidaButhoideaButhidaeParabuthus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6363KurtoxinPRO_0000066792Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi12 ↔ 611 Publication
Disulfide bondi16 ↔ 371 Publication
Disulfide bondi23 ↔ 441 Publication
Disulfide bondi27 ↔ 461 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

1
63
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 43Combined sources
Beta strandi9 – 113Combined sources
Turni12 – 154Combined sources
Beta strandi18 – 203Combined sources
Helixi22 – 298Combined sources
Beta strandi33 – 375Combined sources
Turni39 – 413Combined sources
Beta strandi44 – 496Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T1TNMR-A1-63[»]
ProteinModelPortaliP58910.
SMRiP58910. Positions 1-62.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP58910.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
IPR018218. Scorpion_toxinL.
IPR002061. Scorpion_toxinL/defensin.
[Graphical view]
PfamiPF00537. Toxin_3. 1 hit.
[Graphical view]
PRINTSiPR00285. SCORPNTOXIN.
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.

Sequencei

Sequence statusi: Complete.

P58910-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
KIDGYPVDYW NCKRICWYNN KYCNDLCKGL KADSGYCWGW TLSCYCQGLP
60
DNARIKRSGR CRA
Length:63
Mass (Da):7,394
Last modified:July 26, 2002 - v1
Checksum:i870B1E47F2DF991C
GO

Mass spectrometryi

Molecular mass is 7386.1 Da from positions 1 - 63. Determined by ESI. 1 Publication

Cross-referencesi

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T1TNMR-A1-63[»]
ProteinModelPortaliP58910.
SMRiP58910. Positions 1-62.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP58910.

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR003614. Scorpion_toxin-like.
IPR018218. Scorpion_toxinL.
IPR002061. Scorpion_toxinL/defensin.
[Graphical view]
PfamiPF00537. Toxin_3. 1 hit.
[Graphical view]
PRINTSiPR00285. SCORPNTOXIN.
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Inhibition of T-type voltage-gated calcium channels by a new scorpion toxin."
    Chuang R.S.-I., Jaffe H., Cribbs L., Perez-Reyes E., Swartz K.J.
    Nat. Neurosci. 1:668-674(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, MASS SPECTROMETRY, FUNCTION.
    Tissue: Venom.
  2. "Kurtoxin, a gating modifier of neuronal high- and low-threshold Ca channels."
    Sidach S.S., Mintz I.M.
    J. Neurosci. 22:2023-2034(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION ON CALCIUM CHANNELS.
  3. "Solution structure of kurtoxin."
    Lee C.W., Min H.J., Cho E.M., Kohno T., Eu Y.J., Kim J.I.
    Submitted (JUN-2005) to the PDB data bank
    Cited for: STRUCTURE BY NMR, DISULFIDE BONDS.

Entry informationi

Entry nameiKURT_PARTR
AccessioniPrimary (citable) accession number: P58910
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 26, 2002
Last sequence update: July 26, 2002
Last modified: October 14, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

This toxin does not affect Cav1.2/CACNA1C, Cav2.1/CACNA1A, Cav2.2/CACNA1B, and Cav2.3/CACNA1E calcium channels.1 Publication

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.