Reviewed, UniProtKB/Swiss-Prot P58893 (CARA_CORGL)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	Cgl1610, cg1814

Organism

Corynebacterium glutamicum (Brevibacterium flavum) [Complete proteome] [HAMAP]

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity. HAMAP MF_01209
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112271
Regions
	Domain	195 – 390	196	Glutamine amidotransferase type-1
	Region	1 – 194	194	CPSase HAMAP MF_01209
Sites
	Active site	273	1	Nucleophile By similarity
	Active site	363	1	By similarity
	Active site	365	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P58893-1 [UniParc]. Last modified July 11, 2002. Version 1. Checksum: 96329ACE03A27C95	FASTA	393	42,134
	10 20 30 40 50 60 MSKDTTTYQG VTEIGSVPAY LVLADGRTFT GFGFGAIGTT LGEAVFTTAM TGYQETMTDP 70 80 90 100 110 120 SYHRQIVVAT APQIGNTGWN DEDNESRDGK IWVAGLVIRD LAARVSNWRA TTSLQQEMAG 130 140 150 160 170 180 QGIVGIGGID TRALVRHLRN EGSIAAGIFS GADAQRPVEE LVEIVKNQPA MTGANLSVEV 190 200 210 220 230 240 SADETYVIEA EGEERHTVVA YDLGIKQNTP RRFSARGVRT VIVPAETPFE DIKQYNPSGV 250 260 270 280 290 300 FISNGPGDPA AADVMVDIVR EVLEADIPFF GICFGNQILG RAFGMETYKL KFGHRGINVP 310 320 330 340 350 360 VKNHITGKID ITAQNHGFAL KGEAGQEFET DFGTAIVTHT CLNDGVVEGI ALKSGRAYSV 370 380 390 QYHPEAAAGP NDASPLFDQF VELMDADAQK KGA

References

[1]

"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

[2]

"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000036 Genomic DNA. Translation: BAB99003.1. BX927152 Genomic DNA. Translation: CAF21619.1.
RefSeq	NP_600824.1. YP_225895.1.
3D structure databases
SMR	P58893. Positions 18-387.
ModBase	Search...
Genome annotation databases
GeneID	1019578. 3343549.
GenomeReviews	Gene locus Cgl1610 in contig BA000036_GR.
KEGG	cgb:cg1814. cgl:NCgl1548.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG286341.
OMA	EADIPFF.
Enzyme and pathway databases
BioCyc	CGLU196627:CG1814-MONOMER.
BRENDA	6.3.5.5. 812.
Family and domain databases
HAMAP	MF_01209_B. CPSase_S_chain_B. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase_dom. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_CORGL

Accession

Primary (citable) accession number: P58893

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	July 11, 2002
Last modified:	February 9, 2010
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)