Reviewed, UniProtKB/Swiss-Prot P58839 (G3P_METKA)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase
      Short name=GAPDH
    EC=1.2.1.59
Alternative name(s):
    NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase

Gene names

Name:	gap
Synonyms:	gapA
Ordered Locus Names:	MK0618

Organism

Methanopyrus kandleri [Complete proteome] [HAMAP]

Taxonomic identifier

2320 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanopyri › Methanopyrales › Methanopyraceae › Methanopyrus

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(P)(+) = 3-phospho-D-glyceroyl phosphate + NAD(P)H.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	Glyceraldehyde-3-phosphate dehydrogenase		PRO_0000145724
Regions
	Nucleotide binding	13 – 14	2	NAD By similarity
	Region	147 – 149	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	202 – 203	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	148	1	Nucleophile By similarity
	Binding site	118	1	NAD; via amide nitrogen By similarity
	Binding site	176	1	NAD By similarity
	Binding site	309	1	NAD; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P58839-1 [UniParc]. Last modified May 27, 2002. Version 1. Checksum: F9BCB72702E22E02	FASTA	350	38,367
	10 20 30 40 50 60 MADVSVLING YGTIGKRVAD AVDAQRDMEV LGVVKTSPDY LARLAVEEYG YPLFVPEDRV 70 80 90 100 110 120 ERFEDAGIET EGTVEDVVLN AEDYGLDVVV DCTPEGIGAR NKETLYEKAG VKAIFQGGEE 130 140 150 160 170 180 AEVAEVSFVA QCNYEEALGA DYVRCVSCNT TALCRTLGTL KEEFELGRVY VTIVRRAADP 190 200 210 220 230 240 HQVKKGPINA IAPNPVTVPS HHGPDVKTVM PDIDITTAAV KVPTTLMHMH VVRVELKEEV 250 260 270 280 290 300 TSDDVIDAFE EARRIWVVPH GEGLGSTAEL IELGRDLGRK RYDLYEILVW EESINVEDGV 310 320 330 340 350 LYYMQAVHQE ADVVPENVDA IRAMTELEED PEASMDATDS ALGVLNSPPL

References

[1]

"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens." Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A. Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002) [PubMed: 11930014] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AV19 / DSM 6324 / JCM 9639 / NBRC 100938.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE010355 Genomic DNA. Translation: AAM01833.1.
RefSeq	NP_613903.1.
3D structure databases
HSSP	HSSP built from PDB template 1CF2 based on UniProtKB P10618.
ModBase	Search...
Genome annotation databases
GeneID	1476719.
GenomeReviews	Gene locus MK0618 in contig AE009439_GR.
KEGG	mka:MK0618.
NMPDR	fig|190192.1.peg.616.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P58839.
Enzyme and pathway databases
BioCyc	MKAN190192:MK0618-MON.
Family and domain databases
HAMAP	MF_00559. [Tree]
InterPro	IPR000173. GlycerAld_3-P_DHase. IPR006436. Glyceraldehyde-3-P_DHase_2_arc. [Graphical view]
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
ProDom	PD007761. GAPDH_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01546. GAPDH-II_archae. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P_METKA

Accession

Primary (citable) accession number: P58839

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	May 27, 2002
Last modified:	November 25, 2008
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)