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Protein

Glyceraldehyde-3-phosphate dehydrogenase 2

Gene

gapB

Organism
Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 1 (gapA), Glyceraldehyde-3-phosphate dehydrogenase 2 (gapB)
  2. Phosphoglycerate kinase 2 (pgk2), Phosphoglycerate kinase 1 (pgk1)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (gpmI2), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 (gpmI1), 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei111NAD; via amide nitrogenBy similarity1
Active sitei141NucleophileBy similarity1
Binding sitei169NADBy similarity1
Binding sitei171Glyceraldehyde 3-phosphateBy similarity1
Binding sitei300NAD; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 14NADBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandNAD, NADP

Enzyme and pathway databases

BioCyciMACE188937:G1FZT-3557-MONOMER
UniPathwayiUPA00109; UER00184

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase 2 (EC:1.2.1.59)
Short name:
GAPDH 2
Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase 2
Gene namesi
Name:gapB
Ordered Locus Names:MA_3345
OrganismiMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifieri188937 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanosarcinalesMethanosarcinaceaeMethanosarcina
Proteomesi
  • UP000002487 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001457191 – 335Glyceraldehyde-3-phosphate dehydrogenase 2Add BLAST335

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi188937.MA3345

Structurei

3D structure databases

ProteinModelPortaliP58838
SMRiP58838
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni140 – 142Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni195 – 196Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiarCOG00493 Archaea
COG0057 LUCA
InParanoidiP58838
KOiK00150
OMAiNAIVPNP
OrthoDBiPOG093Z05Z6
PhylomeDBiP58838

Family and domain databases

HAMAPiMF_00559 G3P_dehdrog_arch, 1 hit
InterProiView protein in InterPro
IPR000846 DapB_N
IPR020831 GlycerAld/Erythrose_P_DH
IPR020830 GlycerAld_3-P_DH_AS
IPR020829 GlycerAld_3-P_DH_cat
IPR020828 GlycerAld_3-P_DH_NAD(P)-bd
IPR006436 Glyceraldehyde-3-P_DH_2_arc
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR10836 PTHR10836, 1 hit
PTHR10836:SF76 PTHR10836:SF76, 1 hit
PfamiView protein in Pfam
PF01113 DapB_N, 1 hit
PF02800 Gp_dh_C, 1 hit
PIRSFiPIRSF000149 GAP_DH, 1 hit
SMARTiView protein in SMART
SM00846 Gp_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01546 GAPDH-II_archae, 1 hit
PROSITEiView protein in PROSITE
PS00071 GAPDH, 1 hit

Sequencei

Sequence statusi: Complete.

P58838-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKAKIAVNG YGTIGKRVAD AAQAQDDMEI IGISKTKPNY EAAVAHQLGY
60 70 80 90 100
DIYAPAANLE AFEKAGMPAA GSIEEMVEKA DVIVDCTPGG IGKSNKALYE
110 120 130 140 150
KAGVKAIWQG GEDHELAGYS FNAVANYEGA LGLDFVRVVS CNTTGLCRVI
160 170 180 190 200
YPIDKAFGTK KVRVTLARRA TDPNDIKKGP INAIVPDPIK LPSHHGPDVK
210 220 230 240 250
TVIPHIDITS AAMKIPTTLM HVHTVNMELK NECTAEDVRQ VLGSQSRVRF
260 270 280 290 300
VGQGITSTAE IMEVARDIKR PRNDMWENCV WPDSITMHEG ELYFFQAIHQ
310 320 330
ESIVVPENVD AIRAMMELES DGAKSILKTN KAIGL
Length:335
Mass (Da):36,359
Last modified:May 27, 2002 - v1
Checksum:i33CDD4095B9816A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE010299 Genomic DNA Translation: AAM06714.1
RefSeqiWP_011023275.1, NC_003552.1

Genome annotation databases

EnsemblBacteriaiAAM06714; AAM06714; MA_3345
GeneIDi1475238
KEGGimac:MA_3345

Similar proteinsi

Entry informationi

Entry nameiG3P2_METAC
AccessioniPrimary (citable) accession number: P58838
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: May 27, 2002
Last modified: February 28, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health