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P58838 (G3P2_METAC) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glyceraldehyde-3-phosphate dehydrogenase 2
Short name=GAPDH 2
EC=1.2.1.59
Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase 2
Gene names
Name:gapB
Ordered Locus Names:MA_3345
OrganismMethanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A)
Taxonomic identifier188937 [NCBI]
Taxonomic lineageArchaea › Euryarchaeota › Methanomicrobia › Methanosarcinales › Methanosarcinaceae › Methanosarcina

Protein attributes

Sequence length335 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H. HAMAP MF_00559

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. HAMAP MF_00559

Subunit structure

Homotetramer By similarity. HAMAP MF_00559

Subcellular location

Cytoplasm By similarity HAMAP MF_00559.

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandNAD
NADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processglycolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionNAD binding

Inferred from electronic annotation. Source: InterPro

NADP binding

Inferred from electronic annotation. Source: InterPro

glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 335335Glyceraldehyde-3-phosphate dehydrogenase 2 HAMAP MF_00559
PRO_0000145719

Regions

Nucleotide binding13 – 142NAD By similarity
Region140 – 1423Glyceraldehyde 3-phosphate binding By similarity
Region195 – 1962Glyceraldehyde 3-phosphate binding By similarity

Sites

Active site1411Nucleophile By similarity
Binding site1111NAD; via amide nitrogen By similarity
Binding site1691NAD By similarity
Binding site1711Glyceraldehyde 3-phosphate By similarity
Binding site3001NAD; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
P58838 [UniParc].

Last modified May 27, 2002. Version 1.
Checksum: 33CDD4095B9816A2

FASTA33536,359
        10         20         30         40         50         60 
MAKAKIAVNG YGTIGKRVAD AAQAQDDMEI IGISKTKPNY EAAVAHQLGY DIYAPAANLE 

        70         80         90        100        110        120 
AFEKAGMPAA GSIEEMVEKA DVIVDCTPGG IGKSNKALYE KAGVKAIWQG GEDHELAGYS 

       130        140        150        160        170        180 
FNAVANYEGA LGLDFVRVVS CNTTGLCRVI YPIDKAFGTK KVRVTLARRA TDPNDIKKGP 

       190        200        210        220        230        240 
INAIVPDPIK LPSHHGPDVK TVIPHIDITS AAMKIPTTLM HVHTVNMELK NECTAEDVRQ 

       250        260        270        280        290        300 
VLGSQSRVRF VGQGITSTAE IMEVARDIKR PRNDMWENCV WPDSITMHEG ELYFFQAIHQ 

       310        320        330 
ESIVVPENVD AIRAMMELES DGAKSILKTN KAIGL

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE010299 Genomic DNA. Translation: AAM06714.1.
RefSeqNP_618234.1. NC_003552.1.

3D structure databases

ProteinModelPortalP58838.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1475238.
GenomeReviewsGene locus MA_3345 in contig AE010299_GR.
KEGGmac:MA3345.
NMPDRfig|188937.1.peg.3260.

Phylogenomic databases

HOGENOMHBG392099.
OMAVPSHHGP.
ProtClustDBPRK04207.

Enzyme and pathway databases

BioCycMACE188937:MA3345-MONOMER.

Family and domain databases

HAMAPMF_00559. G3P_dehdrog_arch.
[Tree]
InterProIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006436. Glyceraldehyde-3-P_DH_2_arc.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK00150.
PfamPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000149. GAP_DH. 1 hit.
SMARTSM00846. Gp_dh_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01546. GAPDH-II_archae. 1 hit.
PROSITEPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG3P2_METAC
AccessionPrimary (citable) accession number: P58838
Entry history
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: May 27, 2002
Last modified: November 16, 2011
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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