P58824 (ASNA_FUSNN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--ammonia ligase
EC=6.3.1.1

Alternative name(s):
Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	FN0776

Organism

Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131)

Taxonomic identifier

190304 [NCBI]

Taxonomic lineage

Bacteria › Fusobacteria › Fusobacterales › Fusobacteriaceae › Fusobacterium

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	Aspartate--ammonia ligase HAMAP MF_00555		PRO_0000195876

Sequences

	Sequence		Length	Mass (Da)
	P58824 [UniParc]. Last modified May 27, 2002. Version 1. Checksum: DA002052DD46761E	FASTA	327	37,974
	10 20 30 40 50 60 MAYISSLDIL ETEIAIKKVK DFFESHLSKE LDLLRVSAPL FVIPESGLND NLNGTERPVS 70 80 90 100 110 120 FDTKSGERVE IVHSLAKWKR MALYRYNIEN DKGIYTDMNA IRRDEDTDFI HSYYVDQWDW 130 140 150 160 170 180 EKIISKEDRN EEYLKDVVRK IYSVFKKTEE YITTEYPKLT KKLPEEITFI TAQELENKYP 190 200 210 220 230 240 NLTPKNREHA AAKEYGAIFL MKIGGKLSSG EKHDGRAPDY DDWDLNGDII FNYPLLGIGL 250 260 270 280 290 300 ELSSMGIRVD EKSLDEQLKI ANCEDRRSLP YHQMILNKVL PYTIGGGIGQ SRICMFFLDK 310 320 LHIGEVQASI WSQEVHEICR QMNIKLL

References

[1]

"Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum strain ATCC 25586." Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A., Bhattacharyya A., Bartman A., Gardner W., Grechkin G., Zhu L., Vasieva O., Chu L., Kogan Y., Chaga O., Goltsman E., Bernal A., Larsen N. , D'Souza M., Walunas T., Pusch G., Haselkorn R., Fonstein M., Kyrpides N.C., Overbeek R. J. Bacteriol. 184:2005-2018(2002) [PubMed: 11889109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE009951 Genomic DNA. Translation: AAL94972.1.
RefSeq	NP_603673.1. NC_003454.1.
3D structure databases
ProteinModelPortal	P58824.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	991591.
GenomeReviews	Gene locus FN0776 in contig AE009951_GR.
KEGG	fnu:FN0776.
NMPDR	fig|190304.1.peg.1352.
PATRIC	21951313. VBIFusNuc122357_1339.
Phylogenomic databases
HOGENOM	HBG288146.
OMA	LNDNLNG.
PhylomeDB	P58824.
ProtClustDB	PRK05425.
Enzyme and pathway databases
BioCyc	FNUC190304:FN0776-MONOMER.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004618. AsnA. [Graphical view]
KO	K01914.
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. AsnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_FUSNN

Accession

Primary (citable) accession number: P58824

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	May 27, 2002
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program