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P58818

- PHR_METTM

UniProt

P58818 - PHR_METTM

Protein

Deoxyribodipyrimidine photo-lyase

Gene

phr

Organism
Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 2 (30 Nov 2010)
      Previous versions | rss
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    Functioni

    Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation.

    Catalytic activityi

    Cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA).

    Cofactori

    Binds 1 FAD. The FAD is in its reduced state.
    Binds 1 8-hydroxy-5-deazaflavin (F420) non-covalently per subunit. The F420 is in its oxidized state.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei239 – 2391DNABy similarity

    GO - Molecular functioni

    1. deoxyribodipyrimidine photo-lyase activity Source: UniProtKB-EC
    2. DNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA repair Source: UniProtKB-KW
    2. protein-chromophore linkage Source: UniProtKB-KW

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    Chromophore, DNA-binding, FAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciMMAR79929:GH5J-1301-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Deoxyribodipyrimidine photo-lyase (EC:4.1.99.3)
    Alternative name(s):
    DNA photolyase
    Photoreactivating enzyme
    Gene namesi
    Name:phr
    Ordered Locus Names:MTBMA_c12980
    OrganismiMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum)
    Taxonomic identifieri79929 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter
    ProteomesiUP000000345: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 444444Deoxyribodipyrimidine photo-lyasePRO_0000085118Add
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini20 – 148129Photolyase/cryptochrome alpha/betaAdd
    BLAST

    Sequence similaritiesi

    Belongs to the DNA photolyase class-2 family.Curated

    Phylogenomic databases

    HOGENOMiHOG000016456.
    KOiK01669.
    OMAiKTGKIHN.

    Family and domain databases

    Gene3Di3.40.50.620. 1 hit.
    InterProiIPR008148. DNA_photolyase_2.
    IPR006050. DNA_photolyase_N.
    IPR005101. Photolyase_FAD-bd/Cryptochr_C.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10211. PTHR10211. 1 hit.
    PfamiPF00875. DNA_photolyase. 1 hit.
    PF03441. FAD_binding_7. 1 hit.
    [Graphical view]
    SUPFAMiSSF48173. SSF48173. 1 hit.
    SSF52425. SSF52425. 1 hit.
    TIGRFAMsiTIGR00591. phr2. 1 hit.
    PROSITEiPS01083. DNA_PHOTOLYASES_2_1. 1 hit.
    PS01084. DNA_PHOTOLYASES_2_2. 1 hit.
    PS51645. PHR_CRY_ALPHA_BETA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P58818-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIHDERIRSL NTEKPARDGK YVIYWMQASV RAHWNHALEY AIETANSLHK    50
    PLIVIFGLTD EFPNANSRHY RFLIEGLRDV GDALMKRGAR LVVENERPPS 100
    AVMRYSDEAS AVVVDRGYLD IQKEWVDELA ESLHVPLMQV ESNVIVPVET 150
    ASPKEEYSAG TFRPKITREL ERFMVPLKER RLAVDSLDLD PGPDLNDVTG 200
    KFRASEELEP SIFSGGASEA IRLFDEFLSE KLACFEKYRN DPVKNCLSNM 250
    SPYLHFGHIS PLYLAMKASK TGECPEFLEE LIVRRELSMN FVHYSDNYSS 300
    IRCLPEWAQK TLMEHARDPK EYEYTLREFE EARTHDPYWN AAQKEMVITG 350
    KMHGYMRMYW GKKILEWTDH PERAYDIAIY LNDKYEIDGR DPNGFTGVAW 400
    CFGKHDRAWA EREIFGKVRY MNDRGLERKF RIGEYVRRVQ ELEE 444
    Length:444
    Mass (Da):51,888
    Last modified:November 30, 2010 - v2
    Checksum:i442143277F21894D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001710 Genomic DNA. Translation: ADL58886.1.
    RefSeqiYP_003850199.1. NC_014408.1.

    Genome annotation databases

    EnsemblBacteriaiADL58886; ADL58886; MTBMA_c12980.
    GeneIDi9705006.
    KEGGimmg:MTBMA_c12980.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001710 Genomic DNA. Translation: ADL58886.1 .
    RefSeqi YP_003850199.1. NC_014408.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADL58886 ; ADL58886 ; MTBMA_c12980 .
    GeneIDi 9705006.
    KEGGi mmg:MTBMA_c12980.

    Phylogenomic databases

    HOGENOMi HOG000016456.
    KOi K01669.
    OMAi KTGKIHN.

    Enzyme and pathway databases

    BioCyci MMAR79929:GH5J-1301-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.620. 1 hit.
    InterProi IPR008148. DNA_photolyase_2.
    IPR006050. DNA_photolyase_N.
    IPR005101. Photolyase_FAD-bd/Cryptochr_C.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10211. PTHR10211. 1 hit.
    Pfami PF00875. DNA_photolyase. 1 hit.
    PF03441. FAD_binding_7. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48173. SSF48173. 1 hit.
    SSF52425. SSF52425. 1 hit.
    TIGRFAMsi TIGR00591. phr2. 1 hit.
    PROSITEi PS01083. DNA_PHOTOLYASES_2_1. 1 hit.
    PS01084. DNA_PHOTOLYASES_2_2. 1 hit.
    PS51645. PHR_CRY_ALPHA_BETA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism."
      Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K.
      J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
    2. "Purification and properties of Methanobacterium thermoautotrophicum DNA photolyase."
      Kiener A., Husain I., Sancar A., Walsh C.
      J. Biol. Chem. 264:13880-13887(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-28.
      Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

    Entry informationi

    Entry nameiPHR_METTM
    AccessioniPrimary (citable) accession number: P58818
    Secondary accession number(s): D9PXD8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2002
    Last sequence update: November 30, 2010
    Last modified: October 1, 2014
    This is version 47 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3