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P58818 (PHR_METTM) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Deoxyribodipyrimidine photo-lyase

EC=4.1.99.3
Alternative name(s):
DNA photolyase
Photoreactivating enzyme
Gene names
Name:phr
Ordered Locus Names:MTBMA_c12980
OrganismMethanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum) [Complete proteome] [HAMAP]
Taxonomic identifier79929 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanobacteriaceaeMethanothermobacter

Protein attributes

Sequence length444 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation.

Catalytic activity

Cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA).

Cofactor

Binds 1 FAD. The FAD is in its reduced state.

Binds 1 8-hydroxy-5-deazaflavin (F420) non-covalently per subunit. The F420 is in its oxidized state.

Sequence similarities

Belongs to the DNA photolyase class-2 family.

Contains 1 photolyase/cryptochrome alpha/beta domain.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
   LigandChromophore
DNA-binding
FAD
Flavoprotein
   Molecular functionLyase
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological_processDNA repair

Inferred from electronic annotation. Source: UniProtKB-KW

protein-chromophore linkage

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

deoxyribodipyrimidine photo-lyase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 444444Deoxyribodipyrimidine photo-lyase
PRO_0000085118

Regions

Domain20 – 148129Photolyase/cryptochrome alpha/beta

Sites

Binding site2391DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
P58818 [UniParc].

Last modified November 30, 2010. Version 2.
Checksum: 442143277F21894D

FASTA44451,888
        10         20         30         40         50         60 
MIHDERIRSL NTEKPARDGK YVIYWMQASV RAHWNHALEY AIETANSLHK PLIVIFGLTD 

        70         80         90        100        110        120 
EFPNANSRHY RFLIEGLRDV GDALMKRGAR LVVENERPPS AVMRYSDEAS AVVVDRGYLD 

       130        140        150        160        170        180 
IQKEWVDELA ESLHVPLMQV ESNVIVPVET ASPKEEYSAG TFRPKITREL ERFMVPLKER 

       190        200        210        220        230        240 
RLAVDSLDLD PGPDLNDVTG KFRASEELEP SIFSGGASEA IRLFDEFLSE KLACFEKYRN 

       250        260        270        280        290        300 
DPVKNCLSNM SPYLHFGHIS PLYLAMKASK TGECPEFLEE LIVRRELSMN FVHYSDNYSS 

       310        320        330        340        350        360 
IRCLPEWAQK TLMEHARDPK EYEYTLREFE EARTHDPYWN AAQKEMVITG KMHGYMRMYW 

       370        380        390        400        410        420 
GKKILEWTDH PERAYDIAIY LNDKYEIDGR DPNGFTGVAW CFGKHDRAWA EREIFGKVRY 

       430        440 
MNDRGLERKF RIGEYVRRVQ ELEE 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism."
Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K.
J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
[2]"Purification and properties of Methanobacterium thermoautotrophicum DNA photolyase."
Kiener A., Husain I., Sancar A., Walsh C.
J. Biol. Chem. 264:13880-13887(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-28.
Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001710 Genomic DNA. Translation: ADL58886.1.
RefSeqYP_003850199.1. NC_014408.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADL58886; ADL58886; MTBMA_c12980.
GeneID9705006.
KEGGmmg:MTBMA_c12980.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000016456.
KOK01669.
OMATAHAYRR.

Enzyme and pathway databases

BioCycMMAR79929:GH5J-1301-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
InterProIPR008148. DNA_photolyase_2.
IPR006050. DNA_photolyase_N.
IPR005101. Photolyase_FAD-bd/Cryptochr_C.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10211. PTHR10211. 1 hit.
PfamPF00875. DNA_photolyase. 1 hit.
PF03441. FAD_binding_7. 1 hit.
[Graphical view]
SUPFAMSSF48173. SSF48173. 1 hit.
SSF52425. SSF52425. 1 hit.
TIGRFAMsTIGR00591. phr2. 1 hit.
PROSITEPS01083. DNA_PHOTOLYASES_2_1. 1 hit.
PS01084. DNA_PHOTOLYASES_2_2. 1 hit.
PS51645. PHR_CRY_ALPHA_BETA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePHR_METTM
AccessionPrimary (citable) accession number: P58818
Secondary accession number(s): D9PXD8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: November 30, 2010
Last modified: October 16, 2013
This is version 45 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families