P58818 (PHR_METTM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyribodipyrimidine photo-lyase
EC=4.1.99.3

Alternative name(s):
DNA photolyase
Photoreactivating enzyme

Gene names

Name:	phr
Ordered Locus Names:	MTBMA_c12980

Organism

Methanothermobacter marburgensis (strain DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg) (Methanobacterium thermoautotrophicum) [Complete proteome] [HAMAP]

Taxonomic identifier

79929 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter › Methanothermobacter marburgensis

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ultraviolet radiation.
Catalytic activity	Cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA).
Cofactor	Binds 1 FAD. The FAD is in its reduced state. Binds 1 8-hydroxy-5-deazaflavin (F420) non-covalently per subunit. The F420 is in its oxidized state.
Sequence similarities	Belongs to the DNA photolyase class-2 family. Contains 1 photolyase/cryptochrome alpha/beta domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Ligand	Chromophore DNA-binding FAD Flavoprotein
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW deoxyribodipyrimidine photo-lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Deoxyribodipyrimidine photo-lyase		PRO_0000085118
Regions
	Domain	20 – 148	129	Photolyase/cryptochrome alpha/beta
Sites
	Binding site	239	1	DNA By similarity

Sequences

	Sequence		Length	Mass (Da)
	P58818 [UniParc]. Last modified November 30, 2010. Version 2. Checksum: 442143277F21894D	FASTA	444	51,888
	10 20 30 40 50 60 MIHDERIRSL NTEKPARDGK YVIYWMQASV RAHWNHALEY AIETANSLHK PLIVIFGLTD 70 80 90 100 110 120 EFPNANSRHY RFLIEGLRDV GDALMKRGAR LVVENERPPS AVMRYSDEAS AVVVDRGYLD 130 140 150 160 170 180 IQKEWVDELA ESLHVPLMQV ESNVIVPVET ASPKEEYSAG TFRPKITREL ERFMVPLKER 190 200 210 220 230 240 RLAVDSLDLD PGPDLNDVTG KFRASEELEP SIFSGGASEA IRLFDEFLSE KLACFEKYRN 250 260 270 280 290 300 DPVKNCLSNM SPYLHFGHIS PLYLAMKASK TGECPEFLEE LIVRRELSMN FVHYSDNYSS 310 320 330 340 350 360 IRCLPEWAQK TLMEHARDPK EYEYTLREFE EARTHDPYWN AAQKEMVITG KMHGYMRMYW 370 380 390 400 410 420 GKKILEWTDH PERAYDIAIY LNDKYEIDGR DPNGFTGVAW CFGKHDRAWA EREIFGKVRY 430 440 MNDRGLERKF RIGEYVRRVQ ELEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Methanothermobacter marburgensis, a methanoarchaeon model organism." Liesegang H., Kaster A.K., Wiezer A., Goenrich M., Wollherr A., Seedorf H., Gottschalk G., Thauer R.K. J. Bacteriol. 192:5850-5851(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.
[2]	"Purification and properties of Methanobacterium thermoautotrophicum DNA photolyase." Kiener A., Husain I., Sancar A., Walsh C. J. Biol. Chem. 264:13880-13887(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-28. Strain: DSM 2133 / 14651 / NBRC 100331 / OCM 82 / Marburg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001710 Genomic DNA. Translation: ADL58886.1.
RefSeq	YP_003850199.1. NC_014408.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ADL58886; ADL58886; MTBMA_c12980.
GeneID	9705006.
KEGG	mmg:MTBMA_c12980.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000016456.
KO	K01669.
OMA	TAHAYRR.
Enzyme and pathway databases
BioCyc	MMAR79929:GH5J-1305-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 1 hit.
InterPro	IPR008148. DNA_photolyase_2. IPR006050. DNA_photolyase_N. IPR005101. Photolyase_FAD-bd/Cryptochr_C. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR10211. PTHR10211. 1 hit.
Pfam	PF00875. DNA_photolyase. 1 hit. PF03441. FAD_binding_7. 1 hit. [Graphical view]
SUPFAM	SSF52425. DNA_photolyase_N. 1 hit. SSF48173. Photolyase_FAD-bd/Cryptochr_C. 1 hit.
TIGRFAMs	TIGR00591. phr2. 1 hit.
PROSITE	PS01083. DNA_PHOTOLYASES_2_1. 1 hit. PS01084. DNA_PHOTOLYASES_2_2. 1 hit. PS51645. PHR_CRY_ALPHA_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PHR_METTM

Accession

Primary (citable) accession number: P58818
Secondary accession number(s): D9PXD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2002
Last sequence update:	November 30, 2010
Last modified:	May 1, 2013
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program