Reviewed, UniProtKB/Swiss-Prot P58816 (MCRZ_METTM)

Names and origin

Protein names

Recommended name:
    Methyl-coenzyme M reductase II subunit gamma
      Short name=MCR II gamma
    EC=2.8.4.1

Gene names

Name:

mrtG

Organism

Methanobacterium thermoautotrophicum (strain Marburg / DSM 2133)

Taxonomic identifier

79929 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanobacteriaceae › Methanothermobacter

Protein attributes

Sequence length	15 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and an heterodisulfide.
Catalytic activity	2-(methylthio)ethanesulfonate (methyl-CoM) + N-(7-mercaptoheptanoyl)threonine 3-O-phosphate (coenzyme B) = CoM-S-S-CoB + methane.
Cofactor	Binds 2 coenzyme F430 noncovalently per hexamer. Coenzyme F430 is a yellow nickel porphinoid.
Pathway	One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.
Subunit structure	Hexamer of two alpha, two beta, and two gamma chains.
Developmental stage	There are two MCR complexes in this bacteria. MCR II is expressed in the early growth phase. Late growth cells contains mostly MCR I.

Ontologies

Keywords
Biological process	Methanogenesis
Molecular function	Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme-B sulfoethylthiotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – ›15	›14	Methyl-coenzyme M reductase II subunit gamma		PRO_0000147481
Experimental info
	Non-terminal residue	15	1

Sequences

	Sequence		Length	Mass (Da)
	P58816-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 97E9439C4223E748	FASTA	15	1,688
	10 MSYKAQYTPG ETQIA

References

[1]	"Two genetically distinct methyl-coenzyme M reductases in Methanobacterium thermoautotrophicum strain Marburg and delta H." Rospert S., Linder D., Ellermann J., Thauer R.K. Eur. J. Biochem. 194:871-877(1990) [PubMed: 2269306] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-15.

Cross-references

Sequence databases
PIR	E69017.
3D structure databases
ModBase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

MCRZ_METTM

Accession

Primary (citable) accession number: P58816

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2002
Last sequence update:	January 23, 2007
Last modified:	September 22, 2009
This is version 30 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)