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Protein

Rho-conotoxin TIA

Gene
N/A
Organism
Conus tulipa (Fish-hunting cone snail) (Tulip cone)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Allosteric inhibitor of alpha-1B adrenergic receptors (ADRA1B). Binds to an allosteric modulatory site on transmembrane helix 6 and 7 at the base of extracellular loop 3 of ADRA1B (PubMed:23184947). Also weekly inhibits alpha-1A (ADRA1A) and alpha-1D (ADRA1D) adrenergic receptors in a competive manner (PubMed:15194691). Potently inhibits contractions of vas deferens, spleen and aorta in response to noradrenaline (PubMed:15680270).5 Publications

Miscellaneous

Has no effect on alpha-2 adrenergic receptors (ADRA2) (PubMed:11528421, PubMed:12824165).2 Publications

GO - Molecular functioni

Keywordsi

Molecular functionG-protein coupled receptor impairing toxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Rho-conotoxin TIA1 Publication
Short name:
Rho-TIA1 Publication
OrganismiConus tulipa (Fish-hunting cone snail) (Tulip cone)
Taxonomic identifieri6495 [NCBI]
Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaGastropodaCaenogastropodaHypsogastropodaNeogastropodaConoideaConidaeConusGastridium

Organism-specific databases

ConoServeri1634 TIA

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi40N → A: Little loss in inhibition. 1 Publication1
Mutagenesisi41W → A: Important loss in inhibition. 1 Publication1
Mutagenesisi42R → A: Most important loss in inhibition. 1 Publication1
Mutagenesisi45L → A: Little loss in inhibition. 1 Publication1
Mutagenesisi46I → A: Important loss in inhibition, and change from non-competitive to competitive antagonist of alpha-1B receptor subtype. 2 Publications1
Mutagenesisi50R → A: Little loss in inhibition. 1 Publication1
Mutagenesisi56F → A or N: Selectivity increase for alpha-1B subtype. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 16Sequence analysisAdd BLAST16
PropeptideiPRO_000040420417 – 381 PublicationAdd BLAST22
PeptideiPRO_000004451039 – 57Rho-conotoxin TIA1 PublicationAdd BLAST19

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi43 ↔ 492 PublicationsImported
Disulfide bondi44 ↔ 572 PublicationsImported
Modified residuei57Cysteine amide1 Publication1

Keywords - PTMi

Amidation, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom duct.1 Publication

Structurei

Secondary structure

158
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi42 – 45Combined sources4
Helixi47 – 50Combined sources4
Helixi54 – 56Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1IENNMR-A39-57[»]
2LR9NMR-A39-57[»]
SMRiP58811
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP58811

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni40 – 42Interaction with ADRA1B3

Domaini

The cysteine framework is I (CC-C-C). Alpha4/7 pattern.Curated

Sequence similaritiesi

Belongs to the conotoxin A superfamily.Curated

Keywords - Domaini

Signal

Family and domain databases

InterProiView protein in InterPro
IPR009958 Conotoxin_a-typ
PfamiView protein in Pfam
PF07365 Toxin_8, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P58811-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFTVFLLVVL ATTGVSFTLD RASDGGNAVA KKSDVTARFN WRCCLIPACR

RNHKKFCG
Length:58
Mass (Da):6,395
Last modified:February 8, 2011 - v2
Checksum:iEAA3234432D865A6
GO

Sequence cautioni

The sequence ADN79119 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Mass spectrometryi

Molecular mass is 2390.15 Da from positions 1 - 19. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
GQ981400 mRNA Translation: ADN79119.1 Different initiation.

Similar proteinsi

Entry informationi

Entry nameiCA1A_CONTU
AccessioniPrimary (citable) accession number: P58811
Secondary accession number(s): E2DEK8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: February 8, 2011
Last modified: April 25, 2018
This is version 62 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

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