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Protein

TBC1 domain family member 10A

Gene

Tbc1d10a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Acts as GTPase-activating protein for RAB27A.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei156 – 1561Arginine fingerBy similarity
Sitei197 – 1971Glutamine fingerBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation, Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
TBC1 domain family member 10A
Alternative name(s):
EBP50-PDX interactor of 64 kDa
Short name:
EPI64 protein
Gene namesi
Name:Tbc1d10a
Synonyms:Epi64, Tbc1d10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2144164. Tbc1d10a.

Subcellular locationi

  • Cell projectionmicrovillus By similarity

  • Note: Localizes to the microvilli-rich region of the syncytiotrophoblast.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 500500TBC1 domain family member 10APRO_0000208036Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei39 – 391PhosphoserineCombined sources
Modified residuei40 – 401PhosphoserineCombined sources
Modified residuei45 – 451PhosphoserineCombined sources
Modified residuei407 – 4071PhosphoserineBy similarity
Modified residuei477 – 4771PhosphothreonineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP58802.
MaxQBiP58802.
PaxDbiP58802.
PRIDEiP58802.

PTM databases

iPTMnetiP58802.
PhosphoSiteiP58802.

Expressioni

Tissue specificityi

Expressed in most tissues, except for skeletal muscle.1 Publication

Gene expression databases

BgeeiP58802.
CleanExiMM_TBC1D10A.
ExpressionAtlasiP58802. baseline and differential.
GenevisibleiP58802. MM.

Interactioni

Subunit structurei

Binds to the first PDZ domain of SLC9A3R1 and SLC9A3R2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000136453.

Structurei

3D structure databases

ProteinModelPortaliP58802.
SMRiP58802. Positions 104-362.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini111 – 299189Rab-GAP TBCPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni497 – 5004Binding to the PDZ domain of EBP50By similarity

Domaini

The arginine and glutamine fingers are critical for the GTPase-activating mechanism, they pull out Rab's 'switch 2' glutamine and insert in Rab's active site.By similarity

Sequence similaritiesi

Contains 1 Rab-GAP TBC domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2221. Eukaryota.
ENOG410XPSR. LUCA.
GeneTreeiENSGT00760000119202.
HOVERGENiHBG070028.
InParanoidiP58802.
KOiK19944.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P58802-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKSSRENGP REPAAGGSLS GTRESLAQGP DAATADELSS LGSDSEANGF
60 70 80 90 100
AERRIDKFGF IVGSQGAEGA LEEVPLEVLR QRESKWLDML NNWDKWMAKK
110 120 130 140 150
HKKIRLRCQK GIPPSLRGRA WQYLSGGKVK LQQNPGKFDE LDMSPGDPKW
160 170 180 190 200
LDVIERDLHR QFPFHEMFVS RGGHGQQDLF RVLKAYTLYR PEEGYCQAQA
210 220 230 240 250
PIAAVLLMHM PAEQAFWCLV QVCEKYLPGY YSEKLEAIQL DGEILFSLLQ
260 270 280 290 300
KVSPVAHKHL SRQKIDPLLY MTEWFMCAFA RTLPWSSVLR VWDMFFCEGV
310 320 330 340 350
KIIFRVGLVL LKHALGSPEK LKACQGQYET IEQLRSLSPK IMQEAFLVQE
360 370 380 390 400
VIELPVTERQ IEREHLIQLR RWQETRGELE CRSLPRMHGA KAILDAEPGP
410 420 430 440 450
RPALQPSPSI RLPPDAALLS SKAKPHKQAQ KEQKRTKTSA QLDKSPGLSQ
460 470 480 490 500
ATVVTAAGDA CPPQGVSPKD PVPQDPTPQN LACHHSQESL TSQESEDTYL
Length:500
Mass (Da):56,202
Last modified:May 2, 2002 - v1
Checksum:i15954DB7A4E3A9B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC018300 mRNA. Translation: AAH18300.1.
CCDSiCCDS56756.1.
RefSeqiNP_598784.1. NM_134023.1.
UniGeneiMm.28140.

Genome annotation databases

EnsembliENSMUST00000180088; ENSMUSP00000136453; ENSMUSG00000034412.
GeneIDi103724.
KEGGimmu:103724.
UCSCiuc007huq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC018300 mRNA. Translation: AAH18300.1.
CCDSiCCDS56756.1.
RefSeqiNP_598784.1. NM_134023.1.
UniGeneiMm.28140.

3D structure databases

ProteinModelPortaliP58802.
SMRiP58802. Positions 104-362.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000136453.

PTM databases

iPTMnetiP58802.
PhosphoSiteiP58802.

Proteomic databases

EPDiP58802.
MaxQBiP58802.
PaxDbiP58802.
PRIDEiP58802.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000180088; ENSMUSP00000136453; ENSMUSG00000034412.
GeneIDi103724.
KEGGimmu:103724.
UCSCiuc007huq.1. mouse.

Organism-specific databases

CTDi83874.
MGIiMGI:2144164. Tbc1d10a.

Phylogenomic databases

eggNOGiKOG2221. Eukaryota.
ENOG410XPSR. LUCA.
GeneTreeiENSGT00760000119202.
HOVERGENiHBG070028.
InParanoidiP58802.
KOiK19944.

Miscellaneous databases

ChiTaRSiTbc1d10a. mouse.
NextBioi356077.
PROiP58802.
SOURCEiSearch...

Gene expression databases

BgeeiP58802.
CleanExiMM_TBC1D10A.
ExpressionAtlasiP58802. baseline and differential.
GenevisibleiP58802. MM.

Family and domain databases

InterProiIPR000195. Rab-GTPase-TBC_dom.
[Graphical view]
PfamiPF00566. RabGAP-TBC. 1 hit.
[Graphical view]
SMARTiSM00164. TBC. 1 hit.
[Graphical view]
SUPFAMiSSF47923. SSF47923. 2 hits.
PROSITEiPS50086. TBC_RABGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Salivary gland.
  2. "Identification of EPI64, a TBC/rabGAP domain-containing microvillar protein that binds to the first PDZ domain of EBP50 and E3KARP."
    Reczek D., Bretscher A.
    J. Cell Biol. 153:191-206(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39; SER-40; SER-45 AND THR-477, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiTB10A_MOUSE
AccessioniPrimary (citable) accession number: P58802
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: May 2, 2002
Last modified: May 11, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.