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Protein

Tropomyosin beta chain

Gene

Tpm2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. The non-muscle isoform may have a role in agonist-mediated receptor internalization.1 Publication

GO - Molecular functioni

  • structural constituent of cytoskeleton Source: MGI

GO - Biological processi

  • muscle contraction Source: MGI
  • regulation of ATPase activity Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Muscle protein

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiR-MMU-390522. Striated Muscle Contraction.
R-MMU-445355. Smooth Muscle Contraction.

Names & Taxonomyi

Protein namesi
Recommended name:
Tropomyosin beta chain
Alternative name(s):
Beta-tropomyosin
Tropomyosin-2
Gene namesi
Name:Tpm2
Synonyms:Tpm-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:98810. Tpm2.

Subcellular locationi

GO - Cellular componenti

  • muscle thin filament tropomyosin Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi61S → A: Abolishes ADRB2 internalization. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002056281 – 284Tropomyosin beta chainAdd BLAST284

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei53PhosphothreonineBy similarity1
Modified residuei61Phosphoserine; by PIK3CG1 Publication1
Cross-linki77Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei79PhosphothreonineBy similarity1
Modified residuei87PhosphoserineBy similarity1
Modified residuei108PhosphothreonineBy similarity1
Modified residuei158PhosphoserineBy similarity1
Modified residuei206PhosphoserineBy similarity1
Modified residuei215PhosphoserineBy similarity1
Modified residuei252PhosphothreonineBy similarity1
Modified residuei261PhosphotyrosineBy similarity1
Modified residuei271PhosphoserineBy similarity1
Modified residuei282PhosphothreonineBy similarity1
Modified residuei283PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on Ser-61 by PIK3CG. Phosphorylation on Ser-61 is required for ADRB2 internalization.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP58774.
PaxDbiP58774.
PeptideAtlasiP58774.
PRIDEiP58774.

2D gel databases

SWISS-2DPAGEP58774.

PTM databases

iPTMnetiP58774.
PhosphoSitePlusiP58774.

Expressioni

Gene expression databases

BgeeiENSMUSG00000028464.
CleanExiMM_TPM2.
ExpressionAtlasiP58774. baseline and differential.
GenevisibleiP58774. MM.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta chain.

Protein-protein interaction databases

BioGridi204292. 4 interactors.
DIPiDIP-628N.
IntActiP58774. 2 interactors.
STRINGi10090.ENSMUSP00000103546.

Structurei

3D structure databases

ProteinModelPortaliP58774.
SMRiP58774.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1 – 284By similarityAdd BLAST284

Domaini

The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.

Sequence similaritiesi

Belongs to the tropomyosin family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1003. Eukaryota.
ENOG410XR5K. LUCA.
GeneTreeiENSGT00550000074494.
HOGENOMiHOG000231521.
HOVERGENiHBG107404.
InParanoidiP58774.
KOiK10374.
OMAiTEPTHEC.
OrthoDBiEOG091G0UO7.
PhylomeDBiP58774.

Family and domain databases

InterProiIPR000533. Tropomyosin.
[Graphical view]
PfamiPF00261. Tropomyosin. 1 hit.
[Graphical view]
PRINTSiPR00194. TROPOMYOSIN.
PROSITEiPS00326. TROPOMYOSIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P58774-1) [UniParc]FASTAAdd to basket
Also known as: Skeletal muscle, TB1-3

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDAIKKKMQM LKLDKENAID RAEQAEADKK QAEDRCKQLE EEQQALQKKL
60 70 80 90 100
KGTEDEVEKY SESVKDAQEK LEQAEKKATD AEADVASLNR RIQLVEEELD
110 120 130 140 150
RAQERLATAL QKLEEAEKAA DESERGMKVI ENRAMKDEEK MELQEMQLKE
160 170 180 190 200
AKHIAEDSDR KYEEVARKLV ILEGELERSE ERAEVAESKC GDLEEELKIV
210 220 230 240 250
TNNLKSLEAQ ADKYSTKEDK YEEEIKLLEE KLKEAETRAE FAERSVAKLE
260 270 280
KTIDDLEDEV YAQKMKYKAI SEELDNALND ITSL
Length:284
Mass (Da):32,837
Last modified:July 21, 1986 - v1
Checksum:iF95D2BF6250F40AE
GO
Isoform 2 (identifier: P58774-2) [UniParc]FASTAAdd to basket
Also known as: non-muscle, Fibroblast, TM-1

The sequence of this isoform differs from the canonical sequence as follows:
     189-213: KCGDLEEELKIVTNNLKSLEAQADK → RARQLEEELRTMDQALKSLIASEEE
     258-284: DEVYAQKMKYKAISEELDNALNDITSL → ETLASAKEENVEIHQTLDQTLLELNNL

Show »
Length:284
Mass (Da):32,958
Checksum:iA35EBB080ADC5DDF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti23 – 24EQ → DE in CAA41271 (PubMed:1733968).Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006597189 – 213KCGDL…AQADK → RARQLEEELRTMDQALKSLI ASEEE in isoform 2. 1 PublicationAdd BLAST25
Alternative sequenceiVSP_006598258 – 284DEVYA…DITSL → ETLASAKEENVEIHQTLDQT LLELNNL in isoform 2. 1 PublicationAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12650 mRNA. Translation: CAA31181.1.
M81086 mRNA. Translation: AAA40484.1.
X58381 mRNA. Translation: CAA41271.1.
CCDSiCCDS18100.1. [P58774-1]
CCDS71374.1. [P58774-2]
PIRiS03838.
S23256.
RefSeqiNP_033442.2. NM_009416.4. [P58774-1]
UniGeneiMm.646.

Genome annotation databases

EnsembliENSMUST00000107913; ENSMUSP00000103546; ENSMUSG00000028464. [P58774-1]
ENSMUST00000107914; ENSMUSP00000103547; ENSMUSG00000028464. [P58774-2]
GeneIDi22004.
KEGGimmu:22004.
UCSCiuc008sqd.2. mouse. [P58774-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12650 mRNA. Translation: CAA31181.1.
M81086 mRNA. Translation: AAA40484.1.
X58381 mRNA. Translation: CAA41271.1.
CCDSiCCDS18100.1. [P58774-1]
CCDS71374.1. [P58774-2]
PIRiS03838.
S23256.
RefSeqiNP_033442.2. NM_009416.4. [P58774-1]
UniGeneiMm.646.

3D structure databases

ProteinModelPortaliP58774.
SMRiP58774.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204292. 4 interactors.
DIPiDIP-628N.
IntActiP58774. 2 interactors.
STRINGi10090.ENSMUSP00000103546.

PTM databases

iPTMnetiP58774.
PhosphoSitePlusiP58774.

2D gel databases

SWISS-2DPAGEP58774.

Proteomic databases

MaxQBiP58774.
PaxDbiP58774.
PeptideAtlasiP58774.
PRIDEiP58774.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000107913; ENSMUSP00000103546; ENSMUSG00000028464. [P58774-1]
ENSMUST00000107914; ENSMUSP00000103547; ENSMUSG00000028464. [P58774-2]
GeneIDi22004.
KEGGimmu:22004.
UCSCiuc008sqd.2. mouse. [P58774-1]

Organism-specific databases

CTDi7169.
MGIiMGI:98810. Tpm2.

Phylogenomic databases

eggNOGiKOG1003. Eukaryota.
ENOG410XR5K. LUCA.
GeneTreeiENSGT00550000074494.
HOGENOMiHOG000231521.
HOVERGENiHBG107404.
InParanoidiP58774.
KOiK10374.
OMAiTEPTHEC.
OrthoDBiEOG091G0UO7.
PhylomeDBiP58774.

Enzyme and pathway databases

ReactomeiR-MMU-390522. Striated Muscle Contraction.
R-MMU-445355. Smooth Muscle Contraction.

Miscellaneous databases

PROiP58774.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028464.
CleanExiMM_TPM2.
ExpressionAtlasiP58774. baseline and differential.
GenevisibleiP58774. MM.

Family and domain databases

InterProiIPR000533. Tropomyosin.
[Graphical view]
PfamiPF00261. Tropomyosin. 1 hit.
[Graphical view]
PRINTSiPR00194. TROPOMYOSIN.
PROSITEiPS00326. TROPOMYOSIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTPM2_MOUSE
AccessioniPrimary (citable) accession number: P58774
Secondary accession number(s): P02560, P46901
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.