P58771 (TPM1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tropomyosin alpha-1 chain

Alternative name(s):
Alpha-tropomyosin
Tropomyosin-1

Gene names

Name:	Tpm1
Synonyms:	Tpm-1, Tpma

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
Subunit structure	Heterodimer of an alpha and a beta chain By similarity. Interacts with HRG (via the HRR domain); the interaction contributes to the antiangiogenic properties of the histidine/proline-rich region (HRR) of HRG By similarity.
Subcellular location	Cytoplasm › cytoskeleton.
Induction	Induced in stimulated quiescent cells. Ref.3
Domain	The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.
Post-translational modification	Phosphorylated at Ser-283 by DAPK1 in response to oxidative stress and this phosphorylation enhances stress fiber formation in endothelial cells By similarity.
Miscellaneous	The sequences of cardiac and skeletal muscles are identical.
Sequence similarities	Belongs to the tropomyosin family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Ligand	Actin-binding
Molecular function	Muscle protein
PTM	Acetylation Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cardiac muscle contraction Inferred from mutant phenotype PubMed 17556658. Source: BHF-UCL cellular response to reactive oxygen species Inferred from electronic annotation. Source: Compara in utero embryonic development Inferred from mutant phenotype PubMed 9440710. Source: MGI positive regulation of heart rate by epinephrine Inferred from mutant phenotype PubMed 17556658. Source: BHF-UCL sarcomere organization Inferred from electronic annotation. Source: Compara ventricular cardiac muscle tissue morphogenesis Inferred from mutant phenotype PubMed 17556658. Source: BHF-UCL
Cellular_component	bleb Inferred from electronic annotation. Source: Compara muscle thin filament tropomyosin Traceable author statement PubMed 8618961. Source: MGI ruffle membrane Inferred from electronic annotation. Source: Compara stress fiber Inferred from electronic annotation. Source: Compara
Molecular_function	structural constituent of cytoskeleton Traceable author statement PubMed 8618961. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: P58771-1)

Also known as: Skeletal muscle;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P58771-2)

Also known as: Fibroblast;

The sequence of this isoform differs from the canonical sequence as follows:
     258-284: DELYAQKLKYKAISEELDHALNDMTSI → EKVAHAKEENLSMHQMLDQTLLELNNM

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Tropomyosin alpha-1 chain		PRO_0000205621
Regions
	Coiled coil	1 – 284	284	By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	283	1	Phosphoserine; alternate Ref.5 Ref.6
	Modified residue	283	1	Phosphoserine; by DAPK1; alternate By similarity
	Cross-link	77		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.4
Natural variations
	Alternative sequence	258 – 284	27	DELYA…DMTSI → EKVAHAKEENLSMHQMLDQT LLELNNM in isoform 2.		VSP_006580

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Skeletal muscle) [UniParc]. Last modified July 21, 1986. Version 1. Checksum: E25609F597A72F4D	FASTA	284	32,681
	10 20 30 40 50 60 MDAIKKKMQM LKLDKENALD RAEQAEADKK AAEDRSKQLE DELVSLQKKL KGTEDELDKY 70 80 90 100 110 120 SEALKDAQEK LELAEKKATD AEADVASLNR RIQLVEEELD RAQERLATAL QKLEEAEKAA 130 140 150 160 170 180 DESERGMKVI ESRAQKDEEK MEIQEIQLKE AKHIAEDADR KYEEVARKLV IIESDLERAE 190 200 210 220 230 240 ERAELSEGKC AELEEELKTV TNNLKSLEAQ AEKYSQKEDK YEEEIKVLSD KLKEAETRAE 250 260 270 280 FAERSVTKLE KSIDDLEDEL YAQKLKYKAI SEELDHALND MTSI
	Isoform 2 (Fibroblast) [UniParc]. Checksum: CD755ABFAEA04540	FASTA	284	32,709
	10 20 30 40 50 60 MDAIKKKMQM LKLDKENALD RAEQAEADKK AAEDRSKQLE DELVSLQKKL KGTEDELDKY 70 80 90 100 110 120 SEALKDAQEK LELAEKKATD AEADVASLNR RIQLVEEELD RAQERLATAL QKLEEAEKAA 130 140 150 160 170 180 DESERGMKVI ESRAQKDEEK MEIQEIQLKE AKHIAEDADR KYEEVARKLV IIESDLERAE 190 200 210 220 230 240 ERAELSEGKC AELEEELKTV TNNLKSLEAQ AEKYSQKEDK YEEEIKVLSD KLKEAETRAE 250 260 270 280 FAERSVTKLE KSIDDLEEKV AHAKEENLSM HQMLDQTLLE LNNM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Subtractive cDNA cloning as a tool to analyse secondary effects of a muscle disease. Characterization of affected genes in the myotonic ADR mouse." Schleef M., Zuehlke C., Schoeffl F., Jockusch H. Neuromuscul. Disord. 4:205-217(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: A2G. Tissue: Fast-twitch skeletal muscle.
[2]	"Isolation and characterization of a cDNA that encodes mouse fibroblast tropomyosin isoform 2." Takenaga K., Nakamura Y., Tokunaga K., Kageyama H., Sakiyama S. Mol. Cell. Biol. 8:5561-5565(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]	"Coordinate induction of fibronectin, fibronectin receptor, tropomyosin, and actin genes in serum-stimulated fibroblasts." Ryseck R.P., MacDonald-Bravo H., Zerial M., Bravo R. Exp. Cell Res. 180:537-545(1989) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[4]	"A proteomics approach to identify the ubiquitinated proteins in mouse heart." Jeon H.B., Choi E.S., Yoon J.H., Hwang J.H., Chang J.W., Lee E.K., Choi H.W., Park Z.-Y., Yoo Y.J. Biochem. Biophys. Res. Commun. 357:731-736(2007) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-77, MASS SPECTROMETRY. Tissue: Heart.
[5]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-283, MASS SPECTROMETRY. Tissue: Liver.
[6]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-283, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X64831 mRNA. Translation: CAA46043.1. M22479 mRNA. Translation: AAA40483.1.
IPI	IPI00123316. IPI00227835.
PIR	A60597. A31380.
RefSeq	NP_001157720.1. NM_001164248.1. NP_077745.2. NM_024427.4.
UniGene	Mm.121878.
3D structure databases
ProteinModelPortal	P58771.
SMR	P58771. Positions 8-284.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-300N.
IntAct	P58771. 6 interactions.
PTM databases
PhosphoSite	P58771.
2D gel databases
SWISS-2DPAGE	P58771.
Proteomic databases
PaxDb	P58771.
PRIDE	P58771.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000113685; ENSMUSP00000109315; ENSMUSG00000032366. ENSMUST00000113707; ENSMUSP00000109337; ENSMUSG00000032366.
GeneID	22003.
KEGG	mmu:22003.
Organism-specific databases
CTD	7168.
MGI	MGI:98809. Tpm1.
Phylogenomic databases
eggNOG	NOG304012.
HOGENOM	HOG000231521.
HOVERGEN	HBG107404.
KO	K10373.
OrthoDB	EOG4TXBSM.
Gene expression databases
ArrayExpress	P58771.
Bgee	P58771.
CleanEx	MM_TPM1.
Genevestigator	P58771.
GermOnline	ENSMUSG00000032366. Mus musculus.
Family and domain databases
InterPro	IPR000533. Tropomyosin. [Graphical view]
Pfam	PF00261. Tropomyosin. 1 hit. [Graphical view]
PRINTS	PR00194. TROPOMYOSIN.
PROSITE	PS00326. TROPOMYOSIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	TPM1. mouse.
NextBio	301732.
SOURCE	Search...

Entry information

Entry name

TPM1_MOUSE

Accession

Primary (citable) accession number: P58771
Secondary accession number(s): P02558 , P19354, P46902, P99034

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	April 3, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program