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Protein

Serine/threonine-protein kinase pim-3

Gene

Pim3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Proto-oncogene with serine/threonine kinase activity that can prevent apoptosis and promote cell survival and protein translation. May contribute to tumorigenesis through: the delivery of survival signaling through phosphorylation of BAD which induces release of the anti-apoptotic protein Bcl-X(L), the regulation of cell cycle progression and protein synthesis and by regulation of MYC transcriptional activity. Additionally to this role on tumorigenesis, can also negatively regulate insulin secretion by inhibiting the activation of MAPK1/3 (ERK1/2), through SOCS6. Involved also in the control of energy metabolism and regulation of AMPK activity in modulating MYC and PPARGC1A protein levels and cell growth.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69ATPPROSITE-ProRule annotation1
Active sitei170Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi46 – 54ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis, Cell cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase pim-3 (EC:2.7.11.1)
Gene namesi
Name:Pim3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1355297. Pim3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mice are viable and fertile, with a profound reduction in body size at birth and throughout postnatal life due to a reduction in the number of cells rather than cell size. Deficient mice have also an increased glucose tolerance.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi38K → R: Enhances insulin secretion after glucose stimulation. 1 Publication1

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000865341 – 326Serine/threonine-protein kinase pim-3Add BLAST326

Post-translational modificationi

Ubiquitinated, leading to proteasomal degradation.By similarity
Phosphorylated. Interaction with PPP2CA promotes dephosphorylation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP58750.
PRIDEiP58750.

PTM databases

iPTMnetiP58750.
PhosphoSitePlusiP58750.

Expressioni

Tissue specificityi

Detected in pancreas but exclusively in beta-cells.1 Publication

Inductioni

By glucose in pancreatic-beta-cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000035828.
CleanExiMM_PIM3.
ExpressionAtlasiP58750. baseline and differential.
GenevisibleiP58750. MM.

Interactioni

Subunit structurei

Interacts with BAD (By similarity). Interacts with PPP2CA; this interaction promotes dephosphorylation of PIM3, ubiquitination and proteasomal degradation (By similarity). Interacts with SOCS6.By similarity1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044603.

Structurei

3D structure databases

ProteinModelPortaliP58750.
SMRiP58750.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini40 – 293Protein kinasePROSITE-ProRule annotationAdd BLAST254

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00760000119045.
HOGENOMiHOG000231357.
HOVERGENiHBG106681.
InParanoidiP58750.
KOiK08807.
OMAiQICDHPW.
OrthoDBiEOG091G0IMW.
PhylomeDBiP58750.
TreeFamiTF320810.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR017348. PIM1/2/3.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF037993. STPK_Pim-1. 1 hit.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P58750-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLSKFGSLA HLCGPGGVDH LPVKILQPAK ADKESFEKVY QVGAVLGSGG
60 70 80 90 100
FGTVYAGSRI ADGLPVAVKH VVKERVTEWG SLGGVAVPLE VVLLRKVGAA
110 120 130 140 150
GGARGVIRLL DWFERPDGFL LVLERPEPAQ DLFDFITERG ALDEPLARRF
160 170 180 190 200
FAQVLAAVRH CHNCGVVHRD IKDENLLVDL RSGELKLIDF GSGAVLKDTV
210 220 230 240 250
YTDFDGTRVY SPPEWIRYHR YHGRSATVWS LGVLLYDMVC GDIPFEQDEE
260 270 280 290 300
ILRGRLFFRR RVSPECQQLI EWCLSLRPSE RPSLDQIAAH PWMLGTEGSV
310 320
PENCDLRLCA LDTDDGASTT SSSESL
Length:326
Mass (Da):35,970
Last modified:March 27, 2002 - v1
Checksum:iDD68CBF46354851E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC017621 mRNA. Translation: AAH17621.1.
BC026639 mRNA. Translation: AAH26639.1.
CCDSiCCDS27734.1.
RefSeqiNP_663453.1. NM_145478.2.
UniGeneiMm.400129.
Mm.474725.

Genome annotation databases

EnsembliENSMUST00000042818; ENSMUSP00000044603; ENSMUSG00000035828.
GeneIDi223775.
KEGGimmu:223775.
UCSCiuc007xeq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC017621 mRNA. Translation: AAH17621.1.
BC026639 mRNA. Translation: AAH26639.1.
CCDSiCCDS27734.1.
RefSeqiNP_663453.1. NM_145478.2.
UniGeneiMm.400129.
Mm.474725.

3D structure databases

ProteinModelPortaliP58750.
SMRiP58750.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044603.

PTM databases

iPTMnetiP58750.
PhosphoSitePlusiP58750.

Proteomic databases

PaxDbiP58750.
PRIDEiP58750.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042818; ENSMUSP00000044603; ENSMUSG00000035828.
GeneIDi223775.
KEGGimmu:223775.
UCSCiuc007xeq.1. mouse.

Organism-specific databases

CTDi415116.
MGIiMGI:1355297. Pim3.

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00760000119045.
HOGENOMiHOG000231357.
HOVERGENiHBG106681.
InParanoidiP58750.
KOiK08807.
OMAiQICDHPW.
OrthoDBiEOG091G0IMW.
PhylomeDBiP58750.
TreeFamiTF320810.

Miscellaneous databases

ChiTaRSiPim3. mouse.
PROiP58750.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035828.
CleanExiMM_PIM3.
ExpressionAtlasiP58750. baseline and differential.
GenevisibleiP58750. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR017348. PIM1/2/3.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF037993. STPK_Pim-1. 1 hit.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPIM3_MOUSE
AccessioniPrimary (citable) accession number: P58750
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 27, 2002
Last modified: November 2, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.