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P58744 (DKGA_SALTI) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Putative 2,5-diketo-D-gluconic acid reductase A
Short name=2,5-DKG reductase A
Short name=2,5-DKGR A
Short name=25DKGR-A
EC=1.1.1.274
Alternative name(s):
AKR5C
Gene names
Name:dkgA
Ordered Locus Names:STY3338, t3085
OrganismSalmonella typhi [Complete proteome] [HAMAP]
Taxonomic identifier90370 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length275 AA.
Sequence statusComplete.
Protein existenceUncertain

General annotation (Comments)

Function

Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) By similarity.

Catalytic activity

2-dehydro-D-gluconate + NADP+ = 2,5-didehydro-D-gluconate + NADPH.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm By similarity.

Miscellaneous

2-keto-L-gulonic acid is a key intermediate in the production of L-ascorbic acid (vitamin C).

Sequence similarities

Belongs to the aldo/keto reductase family.

Caution

Could be the product of a pseudogene.

Sequence caution

The sequence AE014613 differs from that shown. Reason: Erroneous termination at position 175. Translated as Trp.

The sequence AL627277 differs from that shown. Reason: Erroneous termination at position 175. Translated as Trp.

Ontologies

Keywords
   Biological processAscorbate biosynthesis
   Cellular componentCytoplasm
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processL-ascorbic acid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular function2,5-didehydrogluconate reductase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 275275Putative 2,5-diketo-D-gluconic acid reductase A
PRO_0000124601

Regions

Nucleotide binding187 – 24155NADP By similarity

Sites

Active site511Proton donor By similarity
Binding site1071Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P58744 [UniParc].

Last modified December 12, 2006. Version 2.
Checksum: 8620FEB8901540AE

FASTA27530,931
        10         20         30         40         50         60 
MANPTIIRLQ DGNVMPQLGL GVWKASNEEV IAAIHKALEV GYRSIDTATA YQNEEGVGKA 

        70         80         90        100        110        120 
LKAASVAREE LFITTKLWND DQKRPREALQ ESLKKLQLDY LDLYLMHWPV PAIDHYVDAW 

       130        140        150        160        170        180 
KGMIALQKEG LVKSIGVCNF QIHHLQRLID ETGVPPVINQ IELHPLMQQR QLHAWNATHK 

       190        200        210        220        230        240 
IQTESWSPLA QGGEGVFDQK VIRELADKYG KTPAQIVIRW HLDCGLVVIP KSVTPSRIAE 

       250        260        270 
NFAVWDFRLD KDELGEIAKL DQGKRLGPDP DQSGG

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AL627277 Genomic DNA. No translation available.
AE014613 Genomic DNA. No translation available.

3D structure databases

ProteinModelPortalP58744.
SMRP58744. Positions 1-275.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GenomeReviewsGene locus STY3338 in contig AE014613_GR.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG605727.
OMAHDGNLMP.

Family and domain databases

InterProIPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
Gene3DG3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHERPTHR11732. Aldo/ket_red. 1 hit.
PfamPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFPIRSF000097. AKR. 1 hit.
PRINTSPR00069. ALDKETRDTASE.
SUPFAMSSF51430. Aldo/ket_red. 1 hit.
PROSITEPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDKGA_SALTI
AccessionPrimary (citable) accession number: P58744
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: December 12, 2006
Last modified: September 21, 2011
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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