Reviewed, UniProtKB/Swiss-Prot P58744 (DKGA_SALTI)

Names and origin

Protein names

Recommended name:
    Putative 2,5-diketo-D-gluconic acid reductase A
      Short name=2,5-DKG reductase A
      Short name=2,5-DKGR A
      Short name=25DKGR-A
    EC=1.1.1.274
Alternative name(s):
    AKR5C

Gene names

Name:	dkgA
Ordered Locus Names:	STY3338, t3085

Organism

Salmonella typhi [Complete proteome] [HAMAP]

Taxonomic identifier

90370 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella

Protein attributes

Sequence length	275 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Uncertain.

General annotation (Comments)

Function	Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) By similarity.
Catalytic activity	2-dehydro-D-gluconate + NADP+ = 2,5-didehydro-D-gluconate + NADPH.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	2-keto-L-gulonic acid is a key intermediate in the production of L-ascorbic acid (vitamin C).
Sequence similarities	Belongs to the aldo/keto reductase family.
Caution	Could be the product of a pseudogene.
Sequence caution	The sequence AE014613 differs from that shown. Reason: Erroneous termination at position 175. Translated as Trp. The sequence AL627277 differs from that shown. Reason: Erroneous termination at position 175. Translated as Trp.

Ontologies

Keywords
Biological process	Ascorbate biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-ascorbic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2,5-didehydrogluconate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 275	275	Putative 2,5-diketo-D-gluconic acid reductase A		PRO_0000124601
Regions
	Nucleotide binding	187 – 241	55	NADP By similarity
Sites
	Active site	51	1	Proton donor By similarity
	Binding site	107	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P58744-1 [UniParc]. Last modified December 12, 2006. Version 2. Checksum: 8620FEB8901540AE	FASTA	275	30,931
	10 20 30 40 50 60 MANPTIIRLQ DGNVMPQLGL GVWKASNEEV IAAIHKALEV GYRSIDTATA YQNEEGVGKA 70 80 90 100 110 120 LKAASVAREE LFITTKLWND DQKRPREALQ ESLKKLQLDY LDLYLMHWPV PAIDHYVDAW 130 140 150 160 170 180 KGMIALQKEG LVKSIGVCNF QIHHLQRLID ETGVPPVINQ IELHPLMQQR QLHAWNATHK 190 200 210 220 230 240 IQTESWSPLA QGGEGVFDQK VIRELADKYG KTPAQIVIRW HLDCGLVVIP KSVTPSRIAE 250 260 270 NFAVWDFRLD KDELGEIAKL DQGKRLGPDP DQSGG

References

[1]

"Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18." Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J., Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M., Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A., Davis P. , Davies R.M., Dowd L., White N., Farrar J., Feltwell T., Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A., Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:848-852(2001) [PubMed: 11677608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CT18.

[2]

"Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and CT18." Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V., Kodoyianni V., Schwartz D.C., Blattner F.R. J. Bacteriol. 185:2330-2337(2003) [PubMed: 12644504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700931 / Ty2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL627277 Genomic DNA. No translation available. AE014613 Genomic DNA. No translation available.
3D structure databases
HSSP	HSSP built from PDB template 1HW6 based on UniProtKB P06632.
SMR	P58744. Positions 1-275.
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus STY3338 in contig AE014613_GR. Gene locus STY3338 in contig AL513382_GR.
KEGG	stt:t3085. sty:STY3338.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P58744.
Enzyme and pathway databases
BRENDA	1.1.1.274. 3716.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. [Graphical view]
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DKGA_SALTI

Accession

Primary (citable) accession number: P58744

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	December 12, 2006
Last modified:	June 16, 2009
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)