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P58743 (S26A5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Prestin
Alternative name(s):
Solute carrier family 26 member 5
Gene names
Name:SLC26A5
Synonyms:PRES
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length744 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Motor protein that converts auditory stimuli to length changes in outer hair cells and mediates sound amplification in the mammalian hearing organ. Prestin is a bidirectional voltage-to-force converter, it can operate at microsecond rates. It uses cytoplasmic anions as extrinsic voltage sensors, probably chloride and bicarbonate. After binding to a site with millimolar affinity, these anions are translocated across the membrane in response to changes in the transmembrane voltage. They move towards the extracellular surface following hyperpolarization, and towards the cytoplasmic side in response to depolarization. As a consequence, this translocation triggers conformational changes in the protein that ultimately alter its surface area in the plane of the plasma membrane. The area decreases when the anion is near the cytoplasmic face of the membrane (short state), and increases when the ion has crossed the membrane to the outer surface (long state). So, it acts as an incomplete transporter. It swings anions across the membrane, but does not allow these anions to dissociate and escape to the extracellular space. Salicylate, an inhibitor of outer hair cell motility, acts as competitive antagonist at the prestin anion-binding site By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Note: Lateral wall of outer hair cells By similarity.

Involvement in disease

Deafness, autosomal recessive, 61 (DFNB61) [MIM:613865]: A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.
Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.1

Sequence similarities

Belongs to the SLC26A/SulP transporter (TC 2.A.53) family. [View classification]

Contains 1 STAS domain.

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P58743-1)

Also known as: SLC26A5a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P58743-2)

Also known as: SLC26A5b;

The sequence of this isoform differs from the canonical sequence as follows:
     682-685: QVVN → FIQR
     686-744: Missing.
Isoform 3 (identifier: P58743-3)

Also known as: SLC26A5c;

The sequence of this isoform differs from the canonical sequence as follows:
     506-516: PSYKVLGKLPE → FHTEMTRRWRP
     517-744: Missing.
Isoform 4 (identifier: P58743-4)

Also known as: SLC26A5d;

The sequence of this isoform differs from the canonical sequence as follows:
     325-335: LLPPANPDTSL → FHTEMTRRWRP
     336-744: Missing.
Isoform 5 (identifier: P58743-5)

The sequence of this isoform differs from the canonical sequence as follows:
     438-469: Missing.
Note: No experimental confirmation available.
Isoform 6 (identifier: P58743-6)

The sequence of this isoform differs from the canonical sequence as follows:
     438-469: Missing.
     595-595: A → ATQ

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 744744Prestin
PRO_0000080167

Regions

Topological domain1 – 7979Cytoplasmic Potential
Transmembrane80 – 10021Helical; Name=1; Potential
Topological domain101 – 1022Extracellular Potential
Transmembrane103 – 12321Helical; Name=2; Potential
Topological domain124 – 1318Cytoplasmic Potential
Transmembrane132 – 15221Helical; Name=3; Potential
Topological domain153 – 18331Extracellular Potential
Transmembrane184 – 20421Helical; Name=4; Potential
Topological domain205 – 2117Cytoplasmic Potential
Transmembrane212 – 23221Helical; Name=5; Potential
Topological domain233 – 25321Extracellular Potential
Transmembrane254 – 27421Helical; Name=6; Potential
Topological domain275 – 28612Cytoplasmic Potential
Transmembrane287 – 30721Helical; Name=7; Potential
Topological domain308 – 33427Extracellular Potential
Transmembrane335 – 35521Helical; Name=8; Potential
Topological domain356 – 37419Cytoplasmic Potential
Transmembrane375 – 39521Helical; Name=9; Potential
Topological domain396 – 41116Extracellular Potential
Transmembrane412 – 43221Helical; Name=10; Potential
Topological domain433 – 4419Cytoplasmic Potential
Transmembrane442 – 46221Helical; Name=11; Potential
Topological domain463 – 47917Extracellular Potential
Transmembrane480 – 50021Helical; Name=12; Potential
Topological domain501 – 744244Cytoplasmic Potential
Domain525 – 713189STAS

Amino acid modifications

Glycosylation1631N-linked (GlcNAc...) Potential
Glycosylation1661N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence325 – 33511LLPPANPDTSL → FHTEMTRRWRP in isoform 4.
VSP_010190
Alternative sequence336 – 744409Missing in isoform 4.
VSP_010191
Alternative sequence438 – 46932Missing in isoform 5 and isoform 6.
VSP_043153
Alternative sequence506 – 51611PSYKVLGKLPE → FHTEMTRRWRP in isoform 3.
VSP_010192
Alternative sequence517 – 744228Missing in isoform 3.
VSP_010193
Alternative sequence5951A → ATQ in isoform 6.
VSP_047640
Alternative sequence682 – 6854QVVN → FIQR in isoform 2.
VSP_010194
Alternative sequence686 – 74459Missing in isoform 2.
VSP_010195

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (SLC26A5a) [UniParc].

Last modified March 27, 2002. Version 1.
Checksum: 9E64BE6DB2DC065E

FASTA74481,264
        10         20         30         40         50         60 
MDHAEENEIL AATQRYYVER PIFSHPVLQE RLHTKDKVPD SIADKLKQAF TCTPKKIRNI 

        70         80         90        100        110        120 
IYMFLPITKW LPAYKFKEYV LGDLVSGIST GVLQLPQGLA FAMLAAVPPI FGLYSSFYPV 

       130        140        150        160        170        180 
IMYCFLGTSR HISIGPFAVI SLMIGGVAVR LVPDDIVIPG GVNATNGTEA RDALRVKVAM 

       190        200        210        220        230        240 
SVTLLSGIIQ FCLGVCRFGF VAIYLTEPLV RGFTTAAAVH VFTSMLKYLF GVKTKRYSGI 

       250        260        270        280        290        300 
FSVVYSTVAV LQNVKNLNVC SLGVGLMVFG LLLGGKEFNE RFKEKLPAPI PLEFFAVVMG 

       310        320        330        340        350        360 
TGISAGFNLK ESYNVDVVGT LPLGLLPPAN PDTSLFHLVY VDAIAIAIVG FSVTISMAKT 

       370        380        390        400        410        420 
LANKHGYQVD GNQELIALGL CNSIGSLFQT FSISCSLSRS LVQEGTGGKT QLAGCLASLM 

       430        440        450        460        470        480 
ILLVILATGF LFESLPQAVL SAIVIVNLKG MFMQFSDLPF FWRTSKIELT IWLTTFVSSL 

       490        500        510        520        530        540 
FLGLDYGLIT AVIIALLTVI YRTQSPSYKV LGKLPETDVY IDIDAYEEVK EIPGIKIFQI 

       550        560        570        580        590        600 
NAPIYYANSD LYSNALKRKT GVNPAVIMGA RRKAMRKYAK EVGNANMANA TVVKADAEVD 

       610        620        630        640        650        660 
GEDATKPEEE DGEVKYPPIV IKSTFPEEMQ RFMPPGDNVH TVILDFTQVN FIDSVGVKTL 

       670        680        690        700        710        720 
AGIVKEYGDV GIYVYLAGCS AQVVNDLTRN RFFENPALWE LLFHSIHDAV LGSQLREALA 

       730        740 
EQEASAPPSQ EDLEPNATPA TPEA 

« Hide

Isoform 2 (SLC26A5b) [UniParc].

Checksum: EF5682F6B068BEAE
Show »

FASTA68574,844
Isoform 3 (SLC26A5c) [UniParc].

Checksum: B12E4F7437574ED6
Show »

FASTA51656,458
Isoform 4 (SLC26A5d) [UniParc].

Checksum: 49BD1BF137784071
Show »

FASTA33536,949
Isoform 5 [UniParc].

Checksum: DFC2F97FD6F18EC1
Show »

FASTA71277,579
Isoform 6 [UniParc].

Checksum: 0D1EB01E3E0578A4
Show »

FASTA71477,809

References

« Hide 'large scale' references
[1]"Prestin, a cochlear motor protein, is defective in non-syndromic hearing loss."
Liu X.Z., Ouyang X.M., Xia X.J., Zheng J., Pandya A., Li F., Du L.L., Welch K.O., Petit C., Smith R.J.H., Webb B.T., Yan D., Arnos K.S., Corey D., Dallos P., Nance W.E., Chen Z.-Y.
Hum. Mol. Genet. 12:1155-1162(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4), INVOLVEMENT IN DFNB61.
Tissue: Organ of Corti.
[2]"Sequence of an alternatively spliced isoform of prestin (SLC26A5e)."
Mount D.B.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 6).
[3]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
+Additional computationally mapped references.

Web resources

Protein Spotlight

Pump up the volume - Issue 22 of May 2002

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF523354 mRNA. Translation: AAP31417.1.
AY256823 mRNA. Translation: AAP31532.1.
AY256824 mRNA. Translation: AAP31533.1.
AY256825 mRNA. Translation: AAP31534.1.
AY289134 mRNA. Translation: AAP43686.1.
AC004668 Genomic DNA. No translation available.
AC005064 Genomic DNA. No translation available.
AC093701 Genomic DNA. No translation available.
BC100833 mRNA. Translation: AAI00834.1.
RefSeqNP_001161434.1. NM_001167962.1.
NP_945350.1. NM_198999.2.
NP_996766.1. NM_206883.2.
NP_996767.1. NM_206884.2.
NP_996768.1. NM_206885.2.
UniGeneHs.585146.

3D structure databases

ProteinModelPortalP58743.
SMRP58743. Positions 505-718.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000304783.

Protein family/group databases

TCDB2.A.53.2.19. the sulfate permease (sulp) family.

PTM databases

PhosphoSiteP58743.

Polymorphism databases

DMDM20139418.

Proteomic databases

PaxDbP58743.
PRIDEP58743.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000306312; ENSP00000304783; ENSG00000170615. [P58743-1]
ENST00000339444; ENSP00000342396; ENSG00000170615. [P58743-2]
ENST00000356767; ENSP00000349210; ENSG00000170615. [P58743-4]
ENST00000393723; ENSP00000377324; ENSG00000170615. [P58743-6]
ENST00000393730; ENSP00000377331; ENSG00000170615. [P58743-5]
ENST00000393735; ENSP00000377336; ENSG00000170615. [P58743-3]
ENST00000432958; ENSP00000389733; ENSG00000170615. [P58743-5]
GeneID375611.
KEGGhsa:375611.
UCSCuc003vbt.2. human. [P58743-2]
uc003vbu.2. human. [P58743-3]
uc003vbv.2. human. [P58743-4]
uc003vbx.3. human. [P58743-5]
uc003vbz.3. human. [P58743-1]

Organism-specific databases

CTD375611.
GeneCardsGC07M102993.
HGNCHGNC:9359. SLC26A5.
MIM604943. gene.
613865. phenotype.
neXtProtNX_P58743.
Orphanet90636. Autosomal recessive nonsyndromic sensorineural deafness type DFNB.
PharmGKBPA33731.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG0659.
HOGENOMHOG000006546.
HOVERGENHBG000639.
KOK14703.
OrthoDBEOG76T9QT.
PhylomeDBP58743.
TreeFamTF313784.

Gene expression databases

BgeeP58743.
CleanExHS_SLC26A5.
GenevestigatorP58743.

Family and domain databases

Gene3D3.30.750.24. 2 hits.
InterProIPR018045. S04_transporter_CS.
IPR002645. STAS_dom.
IPR001902. SulP_transpt.
IPR011547. Sulph_transpt.
[Graphical view]
PfamPF01740. STAS. 1 hit.
PF00916. Sulfate_transp. 1 hit.
[Graphical view]
SUPFAMSSF52091. SSF52091. 2 hits.
TIGRFAMsTIGR00815. sulP. 1 hit.
PROSITEPS01130. SLC26A. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiPrestin.
GenomeRNAi375611.
NextBio100545.
PROP58743.
SOURCESearch...

Entry information

Entry nameS26A5_HUMAN
AccessionPrimary (citable) accession number: P58743
Secondary accession number(s): Q496J2 expand/collapse secondary AC list , Q7Z7F3, Q86UF8, Q86UF9, Q86UG0
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 27, 2002
Last modified: April 16, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM