P58739 (DADA_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-amino acid dehydrogenase small subunit
EC=1.4.99.1

Gene names

Name:	dadA
Ordered Locus Names:	Atu3293
ORF Names:	AGR_L_3050

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970)

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity. HAMAP MF_01202
Catalytic activity	A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. HAMAP MF_01202
Cofactor	FAD By similarity. HAMAP MF_01202
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1. HAMAP MF_01202
Subunit structure	Heterodimer of a small and a large subunit By similarity.
Sequence similarities	Belongs to the DadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	D-amino acid dehydrogenase small subunit HAMAP MF_01202		PRO_0000166124
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	P58739 [UniParc]. Last modified March 5, 2002. Version 1. Checksum: 6DD2ACAF503BC216	FASTA	418	45,179
	10 20 30 40 50 60 MNVTILGAGV VGVTSAWYLA KAGHKVTVID RQPAAALETS FANAGEVSPG YSSPWAAPGI 70 80 90 100 110 120 PMKAMKWLFM KHAPLIIRPT ADPAAWRWMS QMLRNCTSAR YAINKSRMVR VAEYSRDCLM 130 140 150 160 170 180 ALREETGIDY DQRMQGTLEV FRTQKQFDAI GKDVDVLTAG GVPFEILDRD GCAAIEPGLA 190 200 210 220 230 240 PAKEKIVGGL RLPGDETGDC FMFTTELARM AEEAGVTFLY DTGIMRPIVE GGRIKAVETT 250 260 270 280 290 300 RGLIEADIFV AALGSYSPQF VRQLGLTLPV YPVKGYSITV PIVKEERAPV STVMDETFKV 310 320 330 340 350 360 AITRLGSRIR AGGMAEIAGF SKDLPAARQE TLAHSVEDLF GGAGDQSQAK FWCGLRPMTP 370 380 390 400 410 DGTPVIGATR YSNLYLNTGH GTLGWTMSCG SARVLADLIS GNKPEIDTHD LAISRYAA

References

[1]

"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

[2]

"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007870 Genomic DNA. Translation: AAK90097.1.
PIR	AG2961. G98321.
RefSeq	NP_357312.1. NC_003063.2.
3D structure databases
ProteinModelPortal	P58739.
ModBase	Search...
Protein-protein interaction databases
STRING	P58739.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1135167.
GenomeReviews	Gene locus Atu3293 in contig AE007870_GR.
KEGG	atu:Atu3293.
PATRIC	20816044. VBIAgrTum91616_3134.
Phylogenomic databases
eggNOG	COG0665.
HOGENOM	HBG729204.
OMA	VDRQPAV.
PhylomeDB	P58739.
ProtClustDB	PRK00711.
Enzyme and pathway databases
BioCyc	ATUM176299-1:ATU3293-MONOMER.
Family and domain databases
HAMAP	MF_01202. DadA. [Tree]
InterPro	IPR023080. D-aa_DH_ssu_DadA. IPR006076. FAD-dep_OxRdtase. [Graphical view]
KO	K00285.
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DADA_AGRT5

Accession

Primary (citable) accession number: P58739

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	March 5, 2002
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program