P58737 (ALR2_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase, catabolic
EC=5.1.1.1

Gene names

Name:	dadB
Ordered Locus Names:	Atu3292
ORF Names:	AGR_L_3051

Organism

Agrobacterium tumefaciens (strain C58 / ATCC 33970)

Taxonomic identifier

176299 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Isomerizes L-alanine to D-alanine which is then oxidized to pyruvate by DadA By similarity. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201
Induction	By alanine. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 388	388	Alanine racemase, catabolic HAMAP MF_01201		PRO_0000114493
Sites
	Active site	46	1	Proton acceptor; specific for D-alanine By similarity
	Active site	267	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	46	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P58737 [UniParc]. Last modified March 5, 2002. Version 1. Checksum: 5F6A9FCB73CBED1B	FASTA	388	40,616
	10 20 30 40 50 60 MDMQISRQQA AGGASGHLTI DLGALRDNYL TLAGMAPASQ TAAVVKADAY GLGADVVSQT 70 80 90 100 110 120 LFEAGCRNFF VAHIDEALAL RLRLSAKARI FVLNGLQPGN ETSCAAMAIT PVLNSLEQIA 130 140 150 160 170 180 QWSAHARELG KTLTAAVQID TGMCRLGLSP EELEILSAKQ QLLDGIEIAF VMSHLACADE 190 200 210 220 230 240 PDHVSNAAQL AVMRKAATAF PETPVCFSNS GGIFLGNDYH NALLRPGIAL YGGAPSAAGP 250 260 270 280 290 300 NPMKPVVRLD VAVIQTRTVP AGSLVGYGGS FTASVPTRLA TIAAGYADGL PRSLSNRGAA 310 320 330 340 350 360 WYNGVRLPIA GRVSMDSIIL DISALPEGTL TQGSLVQMIG PDQTLEDIAE DAGTIAYEIL 370 380 TGLGRRYRRS YIQPGMSPAT ASTSVNHK

References

[1]	"The genome of the natural genetic engineer Agrobacterium tumefaciens C58." Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P., Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L., Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr., Chapman P., Clendenning J. , Deatherage G., Gillet W., Grant C., Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A., Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D., Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M., Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M., Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V., Nester E.W. Science 294:2317-2323(2001) [PubMed: 11743193] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.
[2]	"Genome sequence of the plant pathogen and biotechnology agent Agrobacterium tumefaciens C58." Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M., Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L., Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F., Wollam C. , Allinger M., Doughty D., Scott C., Lappas C., Markelz B., Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G., Cielo C., Slater S. Science 294:2323-2328(2001) [PubMed: 11743194] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C58 / ATCC 33970.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE007870 Genomic DNA. Translation: AAK90098.1.
PIR	AF2961. H98321.
RefSeq	NP_357313.1. NC_003063.2.
3D structure databases
ProteinModelPortal	P58737.
ModBase	Search...
Protein-protein interaction databases
STRING	P58737.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1135166.
GenomeReviews	Gene locus Atu3292 in contig AE007870_GR.
KEGG	atu:Atu3292.
PATRIC	20816042. VBIAgrTum91616_3133.
Phylogenomic databases
eggNOG	COG0787.
HOGENOM	HBG712172.
OMA	GHKLPIV.
PhylomeDB	P58737.
Enzyme and pathway databases
BioCyc	ATUM176299-1:ATU3292-MONOMER.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
KO	K01775.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR2_AGRT5

Accession

Primary (citable) accession number: P58737

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	March 5, 2002
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program