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P58718

- PDXA2_SALTY

UniProt

P58718 - PDXA2_SALTY

Protein

4-hydroxythreonine-4-phosphate dehydrogenase 2

Gene

pdxA2

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (31 Jan 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).By similarity

    Catalytic activityi

    4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.

    Cofactori

    Binds 1 divalent metal cation per subunit. Can use ions such as zinc, magnesium or cobalt By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei139 – 1391SubstrateBy similarity
    Binding sitei140 – 1401SubstrateBy similarity
    Metal bindingi169 – 1691Divalent metal cation; shared with dimeric partnerBy similarity
    Metal bindingi213 – 2131Divalent metal cation; shared with dimeric partnerBy similarity
    Metal bindingi268 – 2681Divalent metal cation; shared with dimeric partnerBy similarity
    Binding sitei276 – 2761SubstrateBy similarity
    Binding sitei285 – 2851SubstrateBy similarity
    Binding sitei294 – 2941SubstrateBy similarity

    GO - Molecular functioni

    1. 4-hydroxythreonine-4-phosphate dehydrogenase activity Source: UniProtKB-HAMAP
    2. cobalt ion binding Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP
    4. NAD binding Source: InterPro
    5. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. pyridoxal phosphate biosynthetic process Source: UniProtKB-HAMAP
    2. pyridoxine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Pyridoxine biosynthesis

    Keywords - Ligandi

    Cobalt, Magnesium, Metal-binding, NAD, NADP, Zinc

    Enzyme and pathway databases

    BioCyciRETL1328306-WGS:GSTH-6864-MONOMER.
    SENT99287:GCTI-162-MONOMER.
    UniPathwayiUPA00244; UER00312.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxythreonine-4-phosphate dehydrogenase 2 (EC:1.1.1.262)
    Alternative name(s):
    4-(phosphohydroxy)-L-threonine dehydrogenase 2
    Gene namesi
    Name:pdxA2
    Ordered Locus Names:STM0163
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000001014: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3273274-hydroxythreonine-4-phosphate dehydrogenase 2PRO_0000188828Add
    BLAST

    Proteomic databases

    PaxDbiP58718.
    PRIDEiP58718.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi99287.STM0163.

    Structurei

    Secondary structure

    1
    327
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi6 – 94
    Turni13 – 164
    Helixi17 – 259
    Turni28 – 325
    Beta strandi34 – 396
    Helixi41 – 499
    Beta strandi57 – 637
    Helixi64 – 663
    Beta strandi73 – 786
    Helixi84 – 863
    Helixi94 – 11219
    Beta strandi117 – 1215
    Helixi126 – 1316
    Helixi139 – 1468
    Beta strandi153 – 1575
    Beta strandi162 – 1665
    Helixi172 – 1787
    Helixi181 – 19717
    Beta strandi204 – 2085
    Helixi212 – 2143
    Helixi222 – 2265
    Helixi228 – 2369
    Turni237 – 2393
    Beta strandi241 – 2466
    Helixi248 – 2569
    Beta strandi261 – 2677
    Helixi268 – 27710
    Beta strandi283 – 2886
    Beta strandi290 – 2989
    Turni303 – 3097
    Helixi314 – 32613

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2HI1X-ray2.30A/B1-327[»]
    ProteinModelPortaliP58718.
    SMRiP58718. Positions 2-327.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP58718.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PdxA family.Curated

    Phylogenomic databases

    eggNOGiCOG1995.
    HOGENOMiHOG000221593.
    KOiK00097.
    OMAiISIKLAM.
    OrthoDBiEOG6GN6ZC.
    PhylomeDBiP58718.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    HAMAPiMF_00536. PdxA.
    InterProiIPR024084. IsoPropMal-DH-like_dom.
    IPR005255. PdxA.
    [Graphical view]
    PfamiPF04166. PdxA. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00557. pdxA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P58718-1 [UniParc]FASTAAdd to Basket

    « Hide

    METKTVAITM GDPAGIGPEI IVKALSEDGL NGAPLVVIGC LATLKRLQAK    50
    GITPNVELRA IERVAEARFA PGIIHVIDEP LAQPEALEAG KVQAQAGDLA 100
    YRCVKRATEL ALRGDVQAIA TAPLNKEALH LAGHNYPGHT ELLATLTHSR 150
    DYAMVLYTDK LKVIHVSTHI ALRKFLDTLS TARVETVIGI ADTFLKRVGY 200
    VKPRIAVAGV NPHAGENGLF GDEETRILTP AITDARAKGM DVYGPCPPDT 250
    VFLQAYEGQY DMVVAMYHDQ GHIPLKLLGF YDGVNITAGL PFIRTSADHG 300
    TAFDIAWTGK AKSESMAVSI KLAMQLA 327
    Length:327
    Mass (Da):35,065
    Last modified:January 31, 2002 - v1
    Checksum:i03BB6725F1896440
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006468 Genomic DNA. Translation: AAL19127.1.
    RefSeqiNP_459168.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL19127; AAL19127; STM0163.
    GeneIDi1251681.
    KEGGistm:STM0163.
    PATRICi32378607. VBISalEnt20916_0173.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006468 Genomic DNA. Translation: AAL19127.1 .
    RefSeqi NP_459168.1. NC_003197.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2HI1 X-ray 2.30 A/B 1-327 [» ]
    ProteinModelPortali P58718.
    SMRi P58718. Positions 2-327.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 99287.STM0163.

    Proteomic databases

    PaxDbi P58718.
    PRIDEi P58718.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL19127 ; AAL19127 ; STM0163 .
    GeneIDi 1251681.
    KEGGi stm:STM0163.
    PATRICi 32378607. VBISalEnt20916_0173.

    Phylogenomic databases

    eggNOGi COG1995.
    HOGENOMi HOG000221593.
    KOi K00097.
    OMAi ISIKLAM.
    OrthoDBi EOG6GN6ZC.
    PhylomeDBi P58718.

    Enzyme and pathway databases

    UniPathwayi UPA00244 ; UER00312 .
    BioCyci RETL1328306-WGS:GSTH-6864-MONOMER.
    SENT99287:GCTI-162-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P58718.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    HAMAPi MF_00536. PdxA.
    InterProi IPR024084. IsoPropMal-DH-like_dom.
    IPR005255. PdxA.
    [Graphical view ]
    Pfami PF04166. PdxA. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00557. pdxA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LT2 / SGSC1412 / ATCC 700720.
    2. "The structure of a putative 4-hydroxythreonine-4-phosphate dehydrogenase from Salmonella typhimurium."
      Midwest center for structural genomics (MCSG)
      Submitted (AUG-2006) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS), SUBUNIT.

    Entry informationi

    Entry nameiPDXA2_SALTY
    AccessioniPrimary (citable) accession number: P58718
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 31, 2002
    Last sequence update: January 31, 2002
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The active site is located at the dimer interface.By similarity

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3