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Protein

D-threonate 4-phosphate dehydrogenase

Gene

pdxA2

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the NAD-dependent oxidation and subsequent decarboxylation of D-threonate 4-phosphate to produce dihydroxyacetone phosphate (DHAP) (PubMed:27402745). Can also use 4-hydroxy-L-threonine 4-phosphate as substrate (PubMed:27294475, PubMed:27402745).2 Publications

Catalytic activityi

D-threonate 4-phosphate + NAD+ = dihydroxyacetone phosphate + CO2 + NADH.1 Publication

Cofactori

a divalent metal cationBy similarityNote: Binds 1 divalent metal cation per subunit.By similarity

Kineticsi

kcat is 8.9 sec(-1) with D-threonate 4-phosphate as substrate. kcat is 0.37 sec(-1) with 4-hydroxy-L-threonine 4-phosphate as substrate (PubMed:27402745). kcat is 0.06 sec(-1) with 4-hydroxy-L-threonine 4-phosphate as substrate (PubMed:27294475).2 Publications
  1. KM=0.054 mM for D-threonate 4-phosphate1 Publication
  2. KM=0.19 mM for 4-hydroxy-L-threonine 4-phosphate1 Publication
  3. KM=7.24 mM for 4-hydroxy-L-threonine 4-phosphate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei139SubstrateBy similarity1
    Binding sitei140SubstrateBy similarity1
    Metal bindingi169Divalent metal cation; shared with dimeric partnerBy similarity1
    Metal bindingi213Divalent metal cation; shared with dimeric partnerBy similarity1
    Metal bindingi268Divalent metal cation; shared with dimeric partnerBy similarity1
    Binding sitei276SubstrateBy similarity1
    Binding sitei285SubstrateBy similarity1
    Binding sitei294SubstrateBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionOxidoreductase
    Biological processCarbohydrate metabolism
    LigandMetal-binding, NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    D-threonate 4-phosphate dehydrogenase1 Publication (EC:1.1.1.-1 Publication)
    Alternative name(s):
    4-(phosphohydroxy)-L-threonine dehydrogenase1 Publication
    Short name:
    4PHT dehydrogenase1 Publication
    Gene namesi
    Name:pdxA22 Publications
    Ordered Locus Names:STM0163
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
    Proteomesi
    • UP000001014 Componenti: Chromosome

    Pathology & Biotechi

    Disruption phenotypei

    Deletion mutant is unable to use D-threonate as a carbon source.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001888281 – 327D-threonate 4-phosphate dehydrogenaseAdd BLAST327

    Proteomic databases

    PaxDbiP58718.
    PRIDEiP58718.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi99287.STM0163.

    Structurei

    Secondary structure

    1327
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi6 – 9Combined sources4
    Turni13 – 16Combined sources4
    Helixi17 – 25Combined sources9
    Turni28 – 32Combined sources5
    Beta strandi34 – 39Combined sources6
    Helixi41 – 49Combined sources9
    Beta strandi57 – 63Combined sources7
    Helixi64 – 66Combined sources3
    Beta strandi73 – 78Combined sources6
    Helixi84 – 86Combined sources3
    Helixi94 – 112Combined sources19
    Beta strandi117 – 121Combined sources5
    Helixi126 – 131Combined sources6
    Helixi139 – 146Combined sources8
    Beta strandi153 – 157Combined sources5
    Beta strandi162 – 166Combined sources5
    Helixi172 – 178Combined sources7
    Helixi181 – 197Combined sources17
    Beta strandi204 – 208Combined sources5
    Helixi212 – 214Combined sources3
    Helixi222 – 226Combined sources5
    Helixi228 – 236Combined sources9
    Turni237 – 239Combined sources3
    Beta strandi241 – 246Combined sources6
    Helixi248 – 256Combined sources9
    Beta strandi261 – 267Combined sources7
    Helixi268 – 277Combined sources10
    Beta strandi283 – 288Combined sources6
    Beta strandi290 – 298Combined sources9
    Turni303 – 309Combined sources7
    Helixi314 – 326Combined sources13

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2HI1X-ray2.30A/B1-327[»]
    ProteinModelPortaliP58718.
    SMRiP58718.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP58718.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PdxA family. PdxA2 subfamily.Curated

    Phylogenomic databases

    eggNOGiENOG4105CEZ. Bacteria.
    COG1995. LUCA.
    HOGENOMiHOG000221593.
    KOiK00097.
    OMAiGHLYPGH.
    PhylomeDBiP58718.

    Family and domain databases

    InterProiView protein in InterPro
    IPR005255. PdxA.
    PANTHERiPTHR30004. PTHR30004. 1 hit.
    PfamiView protein in Pfam
    PF04166. PdxA. 1 hit.
    TIGRFAMsiTIGR00557. pdxA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P58718-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    METKTVAITM GDPAGIGPEI IVKALSEDGL NGAPLVVIGC LATLKRLQAK
    60 70 80 90 100
    GITPNVELRA IERVAEARFA PGIIHVIDEP LAQPEALEAG KVQAQAGDLA
    110 120 130 140 150
    YRCVKRATEL ALRGDVQAIA TAPLNKEALH LAGHNYPGHT ELLATLTHSR
    160 170 180 190 200
    DYAMVLYTDK LKVIHVSTHI ALRKFLDTLS TARVETVIGI ADTFLKRVGY
    210 220 230 240 250
    VKPRIAVAGV NPHAGENGLF GDEETRILTP AITDARAKGM DVYGPCPPDT
    260 270 280 290 300
    VFLQAYEGQY DMVVAMYHDQ GHIPLKLLGF YDGVNITAGL PFIRTSADHG
    310 320
    TAFDIAWTGK AKSESMAVSI KLAMQLA
    Length:327
    Mass (Da):35,065
    Last modified:January 31, 2002 - v1
    Checksum:i03BB6725F1896440
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE006468 Genomic DNA. Translation: AAL19127.1.
    RefSeqiNP_459168.1. NC_003197.2.
    WP_000448745.1. NC_003197.2.

    Genome annotation databases

    EnsemblBacteriaiAAL19127; AAL19127; STM0163.
    GeneIDi1251681.
    KEGGistm:STM0163.
    PATRICifig|99287.12.peg.173.

    Similar proteinsi

    Entry informationi

    Entry nameiPDXA2_SALTY
    AccessioniPrimary (citable) accession number: P58718
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 31, 2002
    Last sequence update: January 31, 2002
    Last modified: August 30, 2017
    This is version 102 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families