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P58717

- PDXA1_SALTY

UniProt

P58717 - PDXA1_SALTY

Protein

4-hydroxythreonine-4-phosphate dehydrogenase 1

Gene

pdxA1

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (31 Jan 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP).By similarity

    Catalytic activityi

    4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH.

    Cofactori

    Binds 1 divalent metal cation per subunit. Can probably use ions such as zinc, magnesium or cobalt.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei136 – 1361SubstrateBy similarity
    Binding sitei137 – 1371SubstrateBy similarity
    Metal bindingi166 – 1661Divalent metal cation; shared with dimeric partner
    Metal bindingi211 – 2111Divalent metal cation; shared with dimeric partner
    Metal bindingi266 – 2661Divalent metal cation; shared with dimeric partner
    Binding sitei274 – 2741SubstrateBy similarity
    Binding sitei283 – 2831SubstrateBy similarity
    Binding sitei292 – 2921SubstrateBy similarity

    GO - Molecular functioni

    1. 4-hydroxythreonine-4-phosphate dehydrogenase activity Source: UniProtKB-HAMAP
    2. cobalt ion binding Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP
    4. NAD binding Source: InterPro
    5. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. pyridoxal phosphate biosynthetic process Source: UniProtKB-HAMAP
    2. pyridoxine biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Pyridoxine biosynthesis

    Keywords - Ligandi

    Cobalt, Magnesium, Metal-binding, NAD, NADP, Zinc

    Enzyme and pathway databases

    BioCyciSENT99287:GCTI-90-MONOMER.
    UniPathwayiUPA00244; UER00312.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    4-hydroxythreonine-4-phosphate dehydrogenase 1 (EC:1.1.1.262)
    Alternative name(s):
    4-(phosphohydroxy)-L-threonine dehydrogenase 1
    Gene namesi
    Name:pdxA1
    Synonyms:pdxA
    Ordered Locus Names:STM0091
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
    ProteomesiUP000001014: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3293294-hydroxythreonine-4-phosphate dehydrogenase 1PRO_0000188826Add
    BLAST

    Proteomic databases

    PaxDbiP58717.
    PRIDEiP58717.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi99287.STM0091.

    Structurei

    Secondary structure

    1
    329
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi6 – 105
    Helixi18 – 258
    Beta strandi31 – 388
    Helixi40 – 4910
    Beta strandi55 – 584
    Beta strandi72 – 776
    Helixi91 – 933
    Helixi94 – 10916
    Beta strandi114 – 1185
    Helixi123 – 1275
    Turni128 – 1303
    Helixi136 – 1438
    Beta strandi150 – 1545
    Beta strandi159 – 1646
    Helixi169 – 1713
    Helixi172 – 1754
    Helixi178 – 19417
    Beta strandi202 – 2065
    Helixi210 – 2167
    Helixi221 – 2244
    Helixi226 – 2349
    Turni235 – 2373
    Beta strandi239 – 2446
    Helixi246 – 2494
    Helixi252 – 2554
    Beta strandi259 – 2657
    Helixi266 – 27712
    Beta strandi282 – 2909
    Beta strandi292 – 2943
    Turni301 – 3066
    Helixi312 – 32716

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1R8KX-ray2.10A/B1-329[»]
    ProteinModelPortaliP58717.
    SMRiP58717. Positions 3-329.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP58717.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PdxA family.Curated

    Phylogenomic databases

    eggNOGiCOG1995.
    HOGENOMiHOG000221592.
    KOiK00097.
    OMAiDTLFQDK.
    OrthoDBiEOG6GN6ZC.
    PhylomeDBiP58717.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    HAMAPiMF_00536. PdxA.
    InterProiIPR024084. IsoPropMal-DH-like_dom.
    IPR005255. PdxA.
    [Graphical view]
    PfamiPF04166. PdxA. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00557. pdxA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P58717-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSAQRVVIT PGEPAGSGPD LVVQLAQRAW PIELVVCADG ALLTERAAML    50
    GLPLSLLPYS PDVPAAPQPA GTLTLLPVSL RAPAISGQLT VENGPYVVET 100
    LARACDGCLN GEFAALITGP VHKGVINDAG ISFTGHTEFF EERSQAKKVV 150
    MMLATEELRV ALATTHLPLR AIADAITPAL LHEVIAILHH DLRTKFGIAE 200
    PRILVCGLNP HAGEGGHMGT EEIDTIIPVL DELRAQGMKL NGPLPADTLF 250
    QPKYLDNADA VLAMYHDQGL PVLKYQGFGR GVNITLGLPF IRTSVDHGTA 300
    LELAGRGKAD VGSFITALNL AIKMIVNTQ 329
    Length:329
    Mass (Da):34,779
    Last modified:January 31, 2002 - v1
    Checksum:i054D9F4E0F34E43B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006468 Genomic DNA. Translation: AAL19055.1.
    RefSeqiNP_459096.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL19055; AAL19055; STM0091.
    GeneIDi1251609.
    KEGGistm:STM0091.
    PATRICi32378449. VBISalEnt20916_0094.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE006468 Genomic DNA. Translation: AAL19055.1 .
    RefSeqi NP_459096.1. NC_003197.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1R8K X-ray 2.10 A/B 1-329 [» ]
    ProteinModelPortali P58717.
    SMRi P58717. Positions 3-329.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 99287.STM0091.

    Proteomic databases

    PaxDbi P58717.
    PRIDEi P58717.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAL19055 ; AAL19055 ; STM0091 .
    GeneIDi 1251609.
    KEGGi stm:STM0091.
    PATRICi 32378449. VBISalEnt20916_0094.

    Phylogenomic databases

    eggNOGi COG1995.
    HOGENOMi HOG000221592.
    KOi K00097.
    OMAi DTLFQDK.
    OrthoDBi EOG6GN6ZC.
    PhylomeDBi P58717.

    Enzyme and pathway databases

    UniPathwayi UPA00244 ; UER00312 .
    BioCyci SENT99287:GCTI-90-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P58717.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    HAMAPi MF_00536. PdxA.
    InterProi IPR024084. IsoPropMal-DH-like_dom.
    IPR005255. PdxA.
    [Graphical view ]
    Pfami PF04166. PdxA. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00557. pdxA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: LT2 / SGSC1412 / ATCC 700720.
    2. "Crystal structure of NAD-dependent dehydrogenase/carboxylase of Salmonella typhimurium."
      Midwest center for structural genomics (MCSG)
      Submitted (JAN-2005) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) IN COMPLEX WITH COBALT IONS, COFACTOR, SUBUNIT.

    Entry informationi

    Entry nameiPDXA1_SALTY
    AccessioniPrimary (citable) accession number: P58717
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 31, 2002
    Last sequence update: January 31, 2002
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The active site is located at the dimer interface.By similarity

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3