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P58714 (PDXA_RALSO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-hydroxythreonine-4-phosphate dehydrogenase

EC=1.1.1.262
Alternative name(s):
4-(phosphohydroxy)-L-threonine dehydrogenase
Gene names
Name:pdxA
Ordered Locus Names:RSc0517
ORF Names:RS04975
OrganismRalstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum) [Complete proteome] [HAMAP]
Taxonomic identifier267608 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeRalstonia

Protein attributes

Sequence length338 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the NAD(P)-dependent oxidation of 4-(phosphohydroxy)-L-threonine (HTP) into 2-amino-3-oxo-4-(phosphohydroxy)butyric acid which spontaneously decarboxylates to form 3-amino-2-oxopropyl phosphate (AHAP) By similarity. HAMAP-Rule MF_00536

Catalytic activity

4-phosphonooxy-L-threonine + NAD+ = 3-amino-2-oxopropyl phosphate + CO2 + NADH. HAMAP-Rule MF_00536

Cofactor

Binds 1 divalent metal cation per subunit. Can use ions such as zinc, magnesium or cobalt By similarity.

Pathway

Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 4/5. HAMAP-Rule MF_00536

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00536

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00536.

Miscellaneous

The active site is located at the dimer interface By similarity.

Sequence similarities

Belongs to the PdxA family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3383384-hydroxythreonine-4-phosphate dehydrogenase HAMAP-Rule MF_00536
PRO_0000188819

Sites

Metal binding1731Divalent metal cation; shared with dimeric partner By similarity
Metal binding2181Divalent metal cation; shared with dimeric partner By similarity
Metal binding2731Divalent metal cation; shared with dimeric partner By similarity
Binding site1361Substrate By similarity
Binding site1371Substrate By similarity
Binding site2811Substrate By similarity
Binding site2901Substrate By similarity
Binding site2991Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P58714 [UniParc].

Last modified January 31, 2002. Version 1.
Checksum: CEC913DD30D77B3E

FASTA33835,592
        10         20         30         40         50         60 
MLNIAITTGE PAGIGPDITV TALLRLLRQP APRHADVRWH VIGDAALLQA RADAIGLGDA 

        70         80         90        100        110        120 
WRDAAAALTV VARPLGAPVR TGVLDAANGR YVLDLLDAAI DGCLPDAAGM ARYDAMVTAP 

       130        140        150        160        170        180 
VQKSTINDAG VPFTGHTEYL AERSRTPRVV MMLAGPQPAH GNAMLRVALA TTHLPLREVP 

       190        200        210        220        230        240 
DAITPAVLDE TLDIVQRDLR ARFGMAAPRI LVTGLNPHAG EAGHLGREEI EVIEPAVVRA 

       250        260        270        280        290        300 
RARGIDARGP YPADTLFQPR LLADADCVLA MYHDQGLAPL KYGTFGHGVN ITLGLPFVRT 

       310        320        330 
SVDHGTALDL AGTGRAEAGS LLEAIDTAVE MARHAAHS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL646052 Genomic DNA. Translation: CAD14045.1.
RefSeqNP_518638.1. NC_003295.1.

3D structure databases

ProteinModelPortalP58714.
SMRP58714. Positions 2-334.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING267608.RSc0517.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD14045; CAD14045; RSc0517.
GeneID1219321.
KEGGrso:RSc0517.
PATRIC20259611. VBIRalSol70888_0539.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1995.
HOGENOMHOG000221592.
KOK00097.
OMAEQNSAYV.
OrthoDBEOG6GN6ZC.

Enzyme and pathway databases

BioCycRSOL267608:GCVU-519-MONOMER.
UniPathwayUPA00244; UER00312.

Family and domain databases

Gene3D3.40.718.10. 1 hit.
HAMAPMF_00536. PdxA.
InterProIPR024084. IsoPropMal-DH-like_dom.
IPR005255. PdxA.
[Graphical view]
PfamPF04166. PdxA. 1 hit.
[Graphical view]
TIGRFAMsTIGR00557. pdxA. 1 hit.
ProtoNetSearch...

Entry information

Entry namePDXA_RALSO
AccessionPrimary (citable) accession number: P58714
Entry history
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: January 31, 2002
Last modified: May 14, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways