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Protein

L-gulonolactone oxidase

Gene

Gulo

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Oxidizes L-gulono-1,4-lactone to hydrogen peroxide and L-xylo-hexulonolactone which spontaneously isomerizes to L-ascorbate.1 Publication

Catalytic activityi

L-gulono-1,4-lactone + O2 = L-ascorbate + H2O2.1 Publication

Cofactori

FAD1 Publication

Pathwayi: L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway

This protein is involved in step 4 of the subpathway that synthesizes L-ascorbate from UDP-alpha-D-glucuronate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. no protein annotated in this organism
  3. Regucalcin (Rgn)
  4. L-gulonolactone oxidase (Gulo)
This subpathway is part of the pathway L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-ascorbate from UDP-alpha-D-glucuronate, the pathway L-ascorbate biosynthesis via UDP-alpha-D-glucuronate pathway and in Cofactor biosynthesis.

GO - Molecular functioni

  • D-arabinono-1,4-lactone oxidase activity Source: InterPro
  • flavin adenine dinucleotide binding Source: UniProtKB
  • L-gulonolactone oxidase activity Source: UniProtKB

GO - Biological processi

  • L-ascorbic acid biosynthetic process Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase
Biological processAscorbate biosynthesis
LigandFAD, Flavoprotein

Enzyme and pathway databases

BRENDAi1.1.3.8. 3474.
UniPathwayiUPA00991; UER00939.

Names & Taxonomyi

Protein namesi
Recommended name:
L-gulonolactone oxidase (EC:1.1.3.8)
Short name:
LGO
Alternative name(s):
L-gulono-gamma-lactone oxidase
Short name:
GLO
Gene namesi
Name:Gulo
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1353434. Gulo.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei251 – 273HelicalSequence analysisAdd BLAST23

GO - Cellular componenti

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001281592 – 440L-gulonolactone oxidaseAdd BLAST439

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei54Pros-8alpha-FAD histidineBy similarity1

Proteomic databases

MaxQBiP58710.
PaxDbiP58710.
PeptideAtlasiP58710.
PRIDEiP58710.

PTM databases

iPTMnetiP58710.
PhosphoSitePlusiP58710.

Expressioni

Tissue specificityi

Highly expressed in liver.1 Publication

Gene expression databases

BgeeiENSMUSG00000034450.
CleanExiMM_GULO.
GenevisibleiP58710. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000060912.

Structurei

3D structure databases

ProteinModelPortaliP58710.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini17 – 187FAD-binding PCMH-typePROSITE-ProRule annotationAdd BLAST171

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4730. Eukaryota.
COG0277. LUCA.
GeneTreeiENSGT00510000049722.
HOGENOMiHOG000252847.
HOVERGENiHBG005834.
InParanoidiP58710.
KOiK00103.
OMAiFRFLWFP.
OrthoDBiEOG091G0FC4.
TreeFamiTF328994.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.40.462.10. 1 hit.
InterProiView protein in InterPro
IPR007173. ALO.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR010031. FAD_lactone_oxidase.
IPR006094. Oxid_FAD_bind_N.
IPR006093. Oxy_OxRdtase_FAD_BS.
IPR030654. Sugar_lactone_oxidase.
IPR016170. V_Alc_oxidase/Cytok_DH_C_dom.
PANTHERiPTHR43762. PTHR43762. 1 hit.
PfamiView protein in Pfam
PF04030. ALO. 1 hit.
PF01565. FAD_binding_4. 1 hit.
PIRSFiPIRSF000136. LGO_GLO. 1 hit.
SUPFAMiSSF56176. SSF56176. 1 hit.
TIGRFAMsiTIGR01678. FAD_lactone_ox. 1 hit.
PROSITEiView protein in PROSITE
PS51387. FAD_PCMH. 1 hit.
PS00862. OX2_COVAL_FAD. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P58710-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVHGYKGVQF QNWAKTYGCS PEMYYQPTSV GEVREVLALA RQQNKKVKVV
60 70 80 90 100
GGGHSPSDIA CTDGFMIHMG KMNRVLQVDK EKKQVTVEAG ILLTDLHPQL
110 120 130 140 150
DKHGLALSNL GAVSDVTVGG VIGSGTHNTG IKHGILATQV VALTLMKADG
160 170 180 190 200
TVLECSESSN ADVFQAARVH LGCLGVILTV TLQCVPQFHL LETSFPSTLK
210 220 230 240 250
EVLDNLDSHL KKSEYFRFLW FPHSENVSII YQDHTNKEPS SASNWFWDYA
260 270 280 290 300
IGFYLLEFLL WTSTYLPRLV GWINRFFFWL LFNCKKESSN LSHKIFSYEC
310 320 330 340 350
RFKQHVQDWA IPREKTKEAL LELKAMLEAH PKVVAHYPVE VRFTRGDDIL
360 370 380 390 400
LSPCFQRDSC YMNIIMYRPY GKDVPRLDYW LAYETIMKKF GGRPHWAKAH
410 420 430 440
NCTRKDFEKM YPAFHKFCDI REKLDPTGMF LNSYLEKVFY
Length:440
Mass (Da):50,478
Last modified:July 27, 2011 - v3
Checksum:iD109B480E08E773B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti160N → K in AAH19856 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY453064 mRNA. Translation: AAR15891.1.
AK077740 mRNA. Translation: BAC36988.1.
AK167460 mRNA. Translation: BAE39545.1.
BC019856 mRNA. Translation: AAH19856.1.
BC028828 mRNA. Translation: AAH28828.1.
CCDSiCCDS36958.1.
RefSeqiNP_848862.1. NM_178747.3.
XP_006519129.1. XM_006519066.1.
UniGeneiMm.26207.

Genome annotation databases

EnsembliENSMUST00000059970; ENSMUSP00000060912; ENSMUSG00000034450.
GeneIDi268756.
KEGGimmu:268756.
UCSCiuc007ujt.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiGGLO_MOUSE
AccessioniPrimary (citable) accession number: P58710
Secondary accession number(s): Q8K152
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: July 27, 2011
Last modified: June 7, 2017
This is version 138 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families