Reviewed, UniProtKB/Swiss-Prot P58710 (GGLO_MOUSE)

Names and origin

Protein names

Recommended name:
    L-gulonolactone oxidase
      Short name=LGO
    EC=1.1.3.8
Alternative name(s):
    L-gulono-gamma-lactone oxidase
      Short name=GLO

Gene names

Name:

Gulo

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Oxidizes L-gulono-1,4-lactone to hydrogen peroxide and L-xylo-hexulonolactone which spontaneously isomerizes to L-ascorbate By similarity.
Catalytic activity	L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2. L-xylo-hex-2-ulono-1,4-lactone = L-ascorbate.
Cofactor	FAD By similarity.
Pathway	Cofactor biosynthesis; L-ascorbic acid biosynthesis.
Subcellular location	Microsome membrane; Single-pass membrane protein By similarity. Endoplasmic reticulum membrane; Single-pass membrane protein By similarity.
Sequence similarities	Belongs to the oxygen-dependent FAD-linked oxidoreductase family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Ascorbate biosynthesis
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	L-ascorbic acid biosynthetic process Inferred from mutant phenotype. Source: MGI oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	D-arabinono-1,4-lactone oxidase activity Inferred from electronic annotation. Source: InterPro FAD binding Inferred from electronic annotation. Source: InterPro L-gulonolactone oxidase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 440	439	L-gulonolactone oxidase		PRO_0000128159
Regions
	Transmembrane	251 – 273	23	Potential
	Domain	17 – 187	171	FAD-binding PCMH-type
Amino acid modifications
	Modified residue	54	1	Tele-8alpha-FAD histidine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P58710-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: D04DB0C1E2D9524B	FASTA	440	50,492
	10 20 30 40 50 60 MVHGYKGVQF QNWAKTYGCS PEMYYQPTSV GEVREVLALA RQQNKKVKVV GGGHSPSDIA 70 80 90 100 110 120 CTDGFMIHMG KMNRVLQVDK EKKQVTVEAG ILLTDLHPQL DKHGLALSNL GAVSDVTVGG 130 140 150 160 170 180 VIGSGTHNTG IKHGILATQV VALTLMKADG TVLECSESSK ADVFQAARVH LGCLGVILTV 190 200 210 220 230 240 TLQCVPQFHL LETSFPSTLK EVLDNLDSHL KKSEYFRFLW FPHSENVSII YQDHTNKEPS 250 260 270 280 290 300 SASNWFWDYA IGFYLLEFLL WTSTYLPRLV GWINRFFFWL LFNCKKESSN LSHKIFSYEC 310 320 330 340 350 360 RFKQHVQDWA IPREKTKEAL LELKAMLEAH PKVVAHYPVE VRFTRGDDIL LSPCFQRDSC 370 380 390 400 410 420 YMNIIMYRPY GKDVPRLDYW LAYETIMKKF GGRPHWAKAH NCTRKDFEKM YPAFHKFCDI 430 440 REKLDPTGMF LNSYLEKVFY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[2]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 318-324, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC019856 mRNA. Translation: AAH19856.1.
IPI	IPI00554830.
UniGene	Mm.26207
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000034450. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:1353434. Gulo.
Phylogenomic databases
HOGENOM	P58710.
HOVERGEN	P58710.
Enzyme and pathway databases
BRENDA	1.1.3.8. 244.
Gene expression databases
ArrayExpress	P58710.
Bgee	P58710.
CleanEx	MM_GULO.
GermOnline	ENSMUSG00000034450. Mus musculus.
Family and domain databases
InterPro	IPR007173. ALO. IPR016166. FAD-bd_2. IPR016168. FAD-linked_Oxase_FAD-bd_sub2. IPR010031. FAD_lactone_oxidase. IPR006094. Oxid_FAD_bind_N. IPR006093. Oxy_OxRdtase_FAD_BS. [Graphical view]
Gene3D	G3DSA:3.30.465.20. FAD-linked_oxidase_FAD-bd_sub2. 1 hit.
Pfam	PF04030. ALO. 1 hit. PF01565. FAD_binding_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR01678. FAD_lactone_ox. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. PS00862. OX2_COVAL_FAD. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

GGLO_MOUSE

Accession

Primary (citable) accession number: P58710

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)