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P58693 (SYN_CLOPE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Asparagine--tRNA ligase
EC=6.1.1.22
Alternative name(s):
Asparaginyl-tRNA synthetase
Short name=AsnRS
Gene names
Name:asnS
Ordered Locus Names:CPE2500
OrganismClostridium perfringens [Complete proteome] [HAMAP]
Taxonomic identifier1502 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length465 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534

Subunit structure

Homodimer By similarity. HAMAP MF_00534

Subcellular location

Cytoplasm HAMAP MF_00534.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processasparaginyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

asparagine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 465465Asparagine--tRNA ligase HAMAP MF_00534
PRO_0000176402

Sequences

Sequence LengthMass (Da)Tools
P58693 [UniParc].

Last modified January 31, 2002. Version 1.
Checksum: C4BAD69916E9A0DC

FASTA46553,168
        10         20         30         40         50         60 
MKDVVLVKSL YRNTSEYSDK KVKISGWIRT LRASNAFGFI EVNDGSFFKN VQVVFDSAKI 

        70         80         90        100        110        120 
SNYKEISKLP ISSSISVIGT LVETPDSKQP FEIQAEEIIV EGMSDSDYPL QKKRHTFEYL 

       130        140        150        160        170        180 
RTIAHLRPRS NAFSATFRVR SVAAYAIHKF FQDQGFVYTH TPILTGSDCE GAGEMFRVTT 

       190        200        210        220        230        240 
LDMMAPPIAE EGGIDFSQDF FGKETNLTVS GQLNAECFAL AFRNIYTFGP TFRAENSNTV 

       250        260        270        280        290        300 
KHAAEFWMIE PEMAFADLID DMEVAENMLK YVIKYVMDEC PEEIAFFNQF VDKGLLERLN 

       310        320        330        340        350        360 
HVVNSEFGKV TYTEAVKLLQ ESGKEFEYPV EWGIDLQTEH ERYLTEQIFK KPVFVTDYPK 

       370        380        390        400        410        420 
DIKAFYMRLN EDGKTVAAMD CLVPGIGEII GGSQREERLD VLKARMAELN LNEEDYWWYL 

       430        440        450        460 
ELRKYGETVH SGFGLGFERL IMYITGMANI RDVIPFPRTT GTAEF

« Hide

References

[1]"Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater."
Shimizu T., Ohtani K., Hirakawa H., Ohshima K., Yamashita A., Shiba T., Ogasawara N., Hattori M., Kuhara S., Hayashi H.
Proc. Natl. Acad. Sci. U.S.A. 99:996-1001(2002) [PubMed: 11792842] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 13 / Type A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000016 Genomic DNA. Translation: BAB82206.1.
RefSeqNP_563416.1. NC_003366.1.

3D structure databases

ProteinModelPortalP58693.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID990878.
GenomeReviewsGene locus CPE2500 in contig BA000016_GR.
KEGGcpe:CPE2500.
NMPDRfig|195102.1.peg.2563.
PATRIC19499089. VBICloPer59675_2569.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG745843.
OMAAIHRFFH.
PhylomeDBP58693.
ProtClustDBPRK03932.

Enzyme and pathway databases

BioCycCPER195102:CPE2500-MONOMER.

Family and domain databases

HAMAPMF_00534. Asn_tRNA_synth.
[Tree]
InterProIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004522. Asn-tRNA-synth_IIb.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR004365. NA-bd_OB_tRNA-helicase.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KOK01893.
PANTHERPTHR22594. aa-tRNA-synt_II. 1 hit.
PTHR22594:SF6. PTHR22594:SF6. 1 hit.
PfamPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti. 1 hit.
[Graphical view]
PRINTSPR01042. TRNASYNTHASP.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMsTIGR00457. AsnS. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYN_CLOPE
AccessionPrimary (citable) accession number: P58693
Entry history
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: January 31, 2002
Last modified: January 25, 2012
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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