P58692 (SYN_NOSS1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Asparagine--tRNA ligase
EC=6.1.1.22

Alternative name(s):
Asparaginyl-tRNA synthetase
Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	alr3658

Organism

Nostoc sp. (strain PCC 7120 / UTEX 2576) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Asparagine--tRNA ligase HAMAP MF_00534		PRO_0000176384

Sequences

	Sequence		Length	Mass (Da)
	P58692 [UniParc]. Last modified January 31, 2002. Version 1. Checksum: B8634A6A5F4A2334	FASTA	463	52,733
	10 20 30 40 50 60 MVNRRIAEIL RSGQPDESLV VQGWVRTKRE LKGFAFIEVN DGSSLGNLQV VINQDLPDYA 70 80 90 100 110 120 VIVKQLNTGA SVEVNGVLVA SQGKGQRIEL KAEAVKVYGE ADPETYPLQK KRHSFEFLRT 130 140 150 160 170 180 IGHLRSRTNS FGAVFRVRNA CSAAIHQFFQ ERGFLWVHTP IITASDCEGA GELFSVTSLD 190 200 210 220 230 240 LKQIPRTENQ GIDYSQDFFA KPTYLTVSGQ LEAEVMAMAF SNVYTFGPTF RAENSNTSRH 250 260 270 280 290 300 LAEFWMVEPE MAFCDLEGDM DLAEAFLKHI FNHVLEKCPE DMEFFNQRID NTVLATAENI 310 320 330 340 350 360 INNQFERLTY TDAIKLLEKA DVKFEYPVSW GLDLQSEHER YLAEQLFKKP VIVTDYPAQI 370 380 390 400 410 420 KAFYMRLSDD EKTVRAMDVL APKIGEIIGG SQREERLDVL ERRVLAQGMQ PEDLWWYLDL 430 440 450 460 RRYGTVPHAG FGLGFERLVQ FITGMGNIRD VIPFPRTPQN AEF

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: PCC 7120 / UTEX 2576.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000019 Genomic DNA. Translation: BAB75357.1.
PIR	AC2263.
RefSeq	NP_487698.1. NC_003272.1.
3D structure databases
ProteinModelPortal	P58692.
ModBase	Search...
Protein-protein interaction databases
STRING	P58692.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1107256.
GenomeReviews	Gene locus alr3658 in contig BA000019_GR.
KEGG	ana:alr3658.
NMPDR	fig|103690.1.peg.3965.
PATRIC	22777772. VBINosSp37423_4340.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0017.
HOGENOM	HBG745843.
OMA	AIHRFFH.
PhylomeDB	P58692.
ProtClustDB	PRK03932.
Enzyme and pathway databases
BioCyc	NSP103690:ALR3658-MONOMER.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01893.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF6. PTHR22594:SF6. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00457. AsnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_NOSS1

Accession

Primary (citable) accession number: P58692

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	January 31, 2002
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program