Reviewed, UniProtKB/Swiss-Prot P58692 (SYN_ANASP)

Names and origin

Protein names

Recommended name:
    Asparaginyl-tRNA synthetase
    EC=6.1.1.22
Alternative name(s):
    Asparagine--tRNA ligase
      Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	alr3658

Organism

Anabaena sp. (strain PCC 7120) [Complete proteome] [HAMAP]

Taxonomic identifier

103690 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Nostocales › Nostocaceae › Nostoc

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00534
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP asparagine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 463	463	Asparaginyl-tRNA synthetase HAMAP MF_00534		PRO_0000176384

Sequences

	Sequence		Length	Mass (Da)
	P58692-1 [UniParc]. Last modified January 31, 2002. Version 1. Checksum: B8634A6A5F4A2334	FASTA	463	52,733
	10 20 30 40 50 60 MVNRRIAEIL RSGQPDESLV VQGWVRTKRE LKGFAFIEVN DGSSLGNLQV VINQDLPDYA 70 80 90 100 110 120 VIVKQLNTGA SVEVNGVLVA SQGKGQRIEL KAEAVKVYGE ADPETYPLQK KRHSFEFLRT 130 140 150 160 170 180 IGHLRSRTNS FGAVFRVRNA CSAAIHQFFQ ERGFLWVHTP IITASDCEGA GELFSVTSLD 190 200 210 220 230 240 LKQIPRTENQ GIDYSQDFFA KPTYLTVSGQ LEAEVMAMAF SNVYTFGPTF RAENSNTSRH 250 260 270 280 290 300 LAEFWMVEPE MAFCDLEGDM DLAEAFLKHI FNHVLEKCPE DMEFFNQRID NTVLATAENI 310 320 330 340 350 360 INNQFERLTY TDAIKLLEKA DVKFEYPVSW GLDLQSEHER YLAEQLFKKP VIVTDYPAQI 370 380 390 400 410 420 KAFYMRLSDD EKTVRAMDVL APKIGEIIGG SQREERLDVL ERRVLAQGMQ PEDLWWYLDL 430 440 450 460 RRYGTVPHAG FGLGFERLVQ FITGMGNIRD VIPFPRTPQN AEF

References

[1]

"Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120." Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M. , Takazawa M., Yamada M., Yasuda M., Tabata S. DNA Res. 8:205-213(2001) [PubMed: 11759840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000019 Genomic DNA. Translation: BAB75357.1.
PIR	AC2263.
RefSeq	NP_487698.1.
3D structure databases
HSSP	HSSP built from PDB template 1BBW based on UniProtKB P13030.
ModBase	Search...
Protein-protein interaction databases
STRING	P58692.
Genome annotation databases
GeneID	1107256.
GenomeReviews	Gene locus alr3658 in contig BA000019_GR.
KEGG	ana:alr3658.
NMPDR	fig|103690.1.peg.3965.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P58692.
OMA	LQKKRHS.
Enzyme and pathway databases
BioCyc	NSP103690:ALR3658-MON.
Family and domain databases
HAMAP	MF_00534. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00457. asnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_ANASP

Accession

Primary (citable) accession number: P58692

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 31, 2002
Last sequence update:	January 31, 2002
Last modified:	November 3, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)