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Protein

Class B acid phosphatase

Gene

aphA

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Dephosphorylates several organic phosphate monoesters such as 3'-UMP, 5'-UMP and pNPP (PubMed:6256351). Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds such as the 2'-, 3-, or 5'-hydroxyls of nucleosides and nucleotides (PubMed:3049613). Also displays significant phosphomutase activity since it is able to catalyze the transfer of the phosphate group of 3'-AMP from the 3'-position both to the 2'- and 5'-positions. One of the physiological functions of the phosphohydrolytic activity of the enzyme is believed to be the scavenging of organic phosphate esters that otherwise cannot pass the cytoplasmic membrane (PubMed:6256351).2 Publications

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.2 Publications

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit.By similarity

Enzyme regulationi

Nucleosides, and particularly 2'-deoxyribonucleosides, are potent inhibitors of the phosphatase activity. The phosphatase activity is also inhibited by inorganic phosphate and EDTA in vitro.2 Publications

Kineticsi

  1. KM=0.30 mM for 5'-UMP (at 37 degrees Celsius and pH 6.5)1 Publication

    pH dependencei

    Optimum pH is 5.0-5.5 and 6.5 for the phosphatase activity with 3'-UMP and 5'-UMP as substrate, respectively.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei69NucleophileBy similarity1
    Metal bindingi69MagnesiumBy similarity1
    Active sitei71Proton donorBy similarity1
    Metal bindingi71Magnesium; via carbonyl oxygenBy similarity1
    Binding sitei177SubstrateBy similarity1
    Metal bindingi192MagnesiumBy similarity1

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    SABIO-RKP58683.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Class B acid phosphatase (EC:3.1.3.22 Publications)
    Short name:
    CBAP
    Alternative name(s):
    Non-specific acid phosphatase II
    Gene namesi
    Name:aphA
    Ordered Locus Names:STM4249
    OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
    Taxonomic identifieri99287 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeSalmonella
    Proteomesi
    • UP000001014 Componenti: Chromosome

    Subcellular locationi

    • Periplasm 1 Publication

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Periplasm

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this gene show a severely decreased growth rate on 5'-UMP as pyrimidine source.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 23Sequence analysisAdd BLAST23
    ChainiPRO_000002400924 – 237Class B acid phosphataseAdd BLAST214

    Proteomic databases

    PaxDbiP58683.
    PRIDEiP58683.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Protein-protein interaction databases

    STRINGi99287.STM4249.

    Structurei

    3D structure databases

    ProteinModelPortaliP58683.
    SMRiP58683.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP58683.

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni137 – 138Substrate bindingBy similarity2

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiENOG4105F0S. Bacteria.
    COG3700. LUCA.
    HOGENOMiHOG000270623.
    KOiK03788.
    OMAiPEFWEKM.
    PhylomeDBiP58683.

    Family and domain databases

    Gene3Di3.40.50.1000. 1 hit.
    InterProiIPR005519. Acid_phosphat_B-like.
    IPR023214. HAD-like_dom.
    IPR010025. HAD-SF_ppase_IIIB_AphA.
    [Graphical view]
    PfamiPF03767. Acid_phosphat_B. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01672. AphA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P58683-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKKITLALSA VCLLFTLNHS ANALVSSPST LNPGTNVAKL AEQAPVHWVS
    60 70 80 90 100
    VAQIENSLTG RPPMAVGFDI DDTVLFSSPG FWRGKKTYSP DSDDYLKNPA
    110 120 130 140 150
    FWEKMNNGWD EFSIPKEVAR QLIDMHVRRG DSIYFVTGRS QTKTETVSKT
    160 170 180 190 200
    LADNFHIPAA NMNPVIFAGD KPEQNTKVQW LQEKNMRIFY GDSDNDITAA
    210 220 230
    RDCGIRGIRI LRAANSTYKP LPQAGAFGEE VIVNSEY
    Length:237
    Mass (Da):26,315
    Last modified:January 31, 2002 - v1
    Checksum:i386E11DC84C16269
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE006468 Genomic DNA. Translation: AAL23073.1.
    RefSeqiNP_463114.1. NC_003197.1.
    WP_000724435.1. NC_003197.1.

    Genome annotation databases

    EnsemblBacteriaiAAL23073; AAL23073; STM4249.
    GeneIDi1255775.
    KEGGistm:STM4249.
    PATRICi32387403. VBISalEnt20916_4469.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE006468 Genomic DNA. Translation: AAL23073.1.
    RefSeqiNP_463114.1. NC_003197.1.
    WP_000724435.1. NC_003197.1.

    3D structure databases

    ProteinModelPortaliP58683.
    SMRiP58683.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi99287.STM4249.

    Proteomic databases

    PaxDbiP58683.
    PRIDEiP58683.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAL23073; AAL23073; STM4249.
    GeneIDi1255775.
    KEGGistm:STM4249.
    PATRICi32387403. VBISalEnt20916_4469.

    Phylogenomic databases

    eggNOGiENOG4105F0S. Bacteria.
    COG3700. LUCA.
    HOGENOMiHOG000270623.
    KOiK03788.
    OMAiPEFWEKM.
    PhylomeDBiP58683.

    Enzyme and pathway databases

    SABIO-RKP58683.

    Miscellaneous databases

    EvolutionaryTraceiP58683.

    Family and domain databases

    Gene3Di3.40.50.1000. 1 hit.
    InterProiIPR005519. Acid_phosphat_B-like.
    IPR023214. HAD-like_dom.
    IPR010025. HAD-SF_ppase_IIIB_AphA.
    [Graphical view]
    PfamiPF03767. Acid_phosphat_B. 1 hit.
    [Graphical view]
    PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
    SUPFAMiSSF56784. SSF56784. 1 hit.
    TIGRFAMsiTIGR01672. AphA. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiAPHA_SALTY
    AccessioniPrimary (citable) accession number: P58683
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 31, 2002
    Last sequence update: January 31, 2002
    Last modified: November 2, 2016
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.