SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P58683

- APHA_SALTY

UniProt

P58683 - APHA_SALTY

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Class B acid phosphatase

Gene
aphA, STM4249
Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Dephosphorylates several organic phosphate monoesters such as 3'-UMP, 5'-UMP and pNPP. Also has a phosphotransferase activity catalyzing the transfer of low-energy phosphate groups from organic phosphate monoesters to free hydroxyl groups of various organic compounds such as the 2'-, 3-, or 5'-hydroxyls of nucleosides and nucleotides. Also displays significant phosphomutase activity since it is able to catalyze the transfer of the phosphate group of 3'-AMP from the 3'-position both to the 2'- and 5'-positions. One of the physiological functions of the phosphohydrolytic activity of the enzyme is believed to be the scavenging of organic phosphate esters that otherwise cannot pass the cytoplasmic membrane.2 Publications

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.2 Publications

Cofactori

Binds 1 magnesium ion per subunit By similarity.

Enzyme regulationi

Nucleosides, and particularly 2'-deoxyribonucleosides, are potent inhibitors of the phosphatase activity. The phosphatase activity is also inhibited by inorganic phosphate and EDTA in vitro.2 Publications

Kineticsi

  1. KM=0.30 mM for 5'-UMP (at 37 degrees Celsius and pH 6.5)1 Publication

pH dependencei

Optimum pH is 5.0-5.5 and 6.5 for the phosphatase activity with 3'-UMP and 5'-UMP as substrate, respectively.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei69 – 691Nucleophile By similarity
Metal bindingi69 – 691Magnesium By similarity
Active sitei71 – 711Proton donor By similarity
Metal bindingi71 – 711Magnesium; via carbonyl oxygen By similarity
Binding sitei177 – 1771Substrate By similarity
Metal bindingi192 – 1921Magnesium By similarity

GO - Molecular functioni

  1. acid phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSENT99287:GCTI-4280-MONOMER.
SABIO-RKP58683.

Names & Taxonomyi

Protein namesi
Recommended name:
Class B acid phosphatase (EC:3.1.3.2)
Short name:
CBAP
Alternative name(s):
Non-specific acid phosphatase II
Gene namesi
Name:aphA
Ordered Locus Names:STM4249
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
ProteomesiUP000001014: Chromosome

Subcellular locationi

Periplasm 1 Publication

GO - Cellular componenti

  1. outer membrane-bounded periplasmic space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene show a severely decreased growth rate on 5'-UMP as pyrimidine source.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323 Reviewed predictionAdd
BLAST
Chaini24 – 237214Class B acid phosphatasePRO_0000024009Add
BLAST

Proteomic databases

PaxDbiP58683.
PRIDEiP58683.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

STRINGi99287.STM4249.

Structurei

3D structure databases

ProteinModelPortaliP58683.
SMRiP58683. Positions 27-237.

Miscellaneous databases

EvolutionaryTraceiP58683.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni137 – 1382Substrate binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3700.
HOGENOMiHOG000270623.
KOiK03788.
OMAiPEFWEKM.
OrthoDBiEOG6HTNVZ.
PhylomeDBiP58683.

Family and domain databases

Gene3Di3.40.50.1000. 1 hit.
InterProiIPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view]
PfamiPF03767. Acid_phosphat_B. 1 hit.
[Graphical view]
PIRSFiPIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01672. AphA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P58683-1 [UniParc]FASTAAdd to Basket

« Hide

MKKITLALSA VCLLFTLNHS ANALVSSPST LNPGTNVAKL AEQAPVHWVS    50
VAQIENSLTG RPPMAVGFDI DDTVLFSSPG FWRGKKTYSP DSDDYLKNPA 100
FWEKMNNGWD EFSIPKEVAR QLIDMHVRRG DSIYFVTGRS QTKTETVSKT 150
LADNFHIPAA NMNPVIFAGD KPEQNTKVQW LQEKNMRIFY GDSDNDITAA 200
RDCGIRGIRI LRAANSTYKP LPQAGAFGEE VIVNSEY 237
Length:237
Mass (Da):26,315
Last modified:January 31, 2002 - v1
Checksum:i386E11DC84C16269
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006468 Genomic DNA. Translation: AAL23073.1.
RefSeqiNP_463114.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL23073; AAL23073; STM4249.
GeneIDi1255775.
KEGGistm:STM4249.
PATRICi32387403. VBISalEnt20916_4469.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE006468 Genomic DNA. Translation: AAL23073.1 .
RefSeqi NP_463114.1. NC_003197.1.

3D structure databases

ProteinModelPortali P58683.
SMRi P58683. Positions 27-237.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 99287.STM4249.

Proteomic databases

PaxDbi P58683.
PRIDEi P58683.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL23073 ; AAL23073 ; STM4249 .
GeneIDi 1255775.
KEGGi stm:STM4249.
PATRICi 32387403. VBISalEnt20916_4469.

Phylogenomic databases

eggNOGi COG3700.
HOGENOMi HOG000270623.
KOi K03788.
OMAi PEFWEKM.
OrthoDBi EOG6HTNVZ.
PhylomeDBi P58683.

Enzyme and pathway databases

BioCyci SENT99287:GCTI-4280-MONOMER.
SABIO-RK P58683.

Miscellaneous databases

EvolutionaryTracei P58683.

Family and domain databases

Gene3Di 3.40.50.1000. 1 hit.
InterProi IPR005519. Acid_phosphat_B.
IPR023214. HAD-like_dom.
IPR010025. HAD-SF_ppase_IIIB_AphA.
[Graphical view ]
Pfami PF03767. Acid_phosphat_B. 1 hit.
[Graphical view ]
PIRSFi PIRSF017818. Acid_Ptase_B. 1 hit.
SUPFAMi SSF56784. SSF56784. 1 hit.
TIGRFAMsi TIGR01672. AphA. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: LT2 / SGSC1412 / ATCC 700720.
  2. "Periplasmic phosphatases in Salmonella typhimurium LT2. A biochemical, physiological, and partial genetic analysis of three nucleoside monophosphate dephosphorylating enzymes."
    Uerkvitz W., Beck C.F.
    J. Biol. Chem. 256:382-389(1981) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A PHOSPHATASE, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, ENZYME REGULATION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    Strain: LT2.
  3. "Periplasmic nonspecific acid phosphatase II from Salmonella typhimurium LT2. Crystallization, detergent reactivation, and phosphotransferase activity."
    Uerkvitz W.
    J. Biol. Chem. 263:15823-15830(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A PHOSPHOTRANSFERASE, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, ENZYME REGULATION, SUBUNIT, CRYSTALLIZATION.
    Strain: LT2.

Entry informationi

Entry nameiAPHA_SALTY
AccessioniPrimary (citable) accession number: P58683
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: January 31, 2002
Last modified: July 9, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi